Construct: sgRNA BRDN0001148182
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCAGAAGACGACTGGCGATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CHKB (1120)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76866
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000022.11 |
22 |
50580394 |
+ |
CHKB |
NNNGAAGACGACTGGCGATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
239216403 |
+ |
HDAC4 |
NNNAAAGACAACTGGCGATG |
NGG |
2 |
0.84 |
Tier II |
| 3 |
NC_000004.12 |
4 |
6951971 |
+ |
TBC1D14 |
NNNGAAGGAGACTGGCGATG |
NGG |
2 |
0.6286 |
Tier II |
| 4 |
NC_000002.12 |
2 |
3806984 |
+ |
DCDC2C |
NNNGAAGATGACTGGCGTTG |
NGG |
2 |
0.4375 |
Tier II |
| 5 |
NC_000022.11 |
22 |
50580394 |
+ |
CHKB-CPT1B |
NNNGAAGACGACTGGCGATG |
NGG |
0 |
1.0 |
Tier III |
| 6 |
NC_000014.9 |
14 |
16055664 |
+ |
LOC107987204 |
NNNGATGCCGACTGGCGATG |
NGG |
2 |
0.3061 |
Tier III |
| 7 |
NC_000014.9 |
14 |
16055664 |
+ |
LOC110467536 |
NNNGATGCCGACTGGCGATG |
NGG |
2 |
0.3061 |
Tier III |
| 8 |
NC_000020.11 |
20 |
30486888 |
- |
LOC102723618 |
NNNGATGCCGACTGGCGATG |
NGG |
2 |
0.3061 |
Tier III |
| 9 |
NC_000020.11 |
20 |
30486888 |
- |
LOC110467535 |
NNNGATGCCGACTGGCGATG |
NGG |
2 |
0.3061 |
Tier III |
| 10 |
NC_000022.11 |
22 |
11251763 |
- |
LOC102723688 |
NNNGATGCCGACTGGCGATG |
NGG |
2 |
0.3061 |
Tier III |
| 11 |
NC_000022.11 |
22 |
11251763 |
- |
LOC110467534 |
NNNGATGCCGACTGGCGATG |
NGG |
2 |
0.3061 |
Tier III |
| 12 |
NC_000022.11 |
22 |
11630644 |
+ |
RNA18SP |
NNNGATGCCGACTGGCGATG |
NGG |
2 |
0.3061 |
Tier III |
| 13 |
NC_000012.12 |
12 |
93016914 |
+ |
LOC643339 |
NNNGAAAACGACTGGCTATG |
NGG |
2 |
0.25 |
Tier III |
| 14 |
NC_000012.12 |
12 |
93016914 |
+ |
LINC02413 |
NNNGAAAACGACTGGCTATG |
NGG |
2 |
0.25 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
96940855 |
+ |
Dscam |
NNNGAAAACGACTGGCGATG |
NGG |
1 |
1.0 |
Tier II |
| 2 |
NC_000082.6 |
16 |
34758660 |
- |
Mylk |
NNNGAAGAGGACTGGAGATG |
NGG |
2 |
0.619 |
Tier II |
| 3 |
NC_000073.6 |
7 |
130245996 |
+ |
Fgfr2 |
NNNGAAGACGACTGGCTTTG |
NGG |
2 |
0.125 |
Tier II |
Other clones with same target sequence:
(none)
