Construct: sgRNA BRDN0001148185
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCCTGGGCCTAACTCCAGAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TAF1L (138474)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77118
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
32633134 |
- |
TAF1L |
NNNTGGGCCTAACTCCAGAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
71387427 |
+ |
TAF1 |
NNNTGGGCCTAACTCCAAAA |
NGG |
1 |
0.6923 |
Tier I |
| 3 |
NC_000003.12 |
3 |
126893902 |
+ |
CHCHD6 |
NNNTGCCCCTAACTCCAGAA |
NGG |
2 |
0.4687 |
Tier II |
| 4 |
NC_000023.11 |
X |
16651981 |
+ |
S100G |
NNNTGGGCTCAACTCCAGAA |
NGG |
2 |
0.4667 |
Tier II |
| 5 |
NC_000023.11 |
X |
16651981 |
+ |
CTPS2 |
NNNTGGGCTCAACTCCAGAA |
NGG |
2 |
0.4667 |
Tier II |
| 6 |
NC_000023.11 |
X |
18642223 |
- |
RS1 |
NNNTGGGGCTAGCTCCAGAA |
NGG |
2 |
0.4643 |
Tier II |
| 7 |
NC_000023.11 |
X |
18642223 |
- |
CDKL5 |
NNNTGGGGCTAGCTCCAGAA |
NGG |
2 |
0.4643 |
Tier II |
| 8 |
NC_000012.12 |
12 |
111059289 |
- |
CUX2 |
NNNTGGGCCCAATTCCAGAA |
NGG |
2 |
0.3733 |
Tier II |
| 9 |
NC_000007.14 |
7 |
130275286 |
- |
CPA2 |
NNNTGGGCCTGACCCCAGAA |
NGG |
2 |
0.1857 |
Tier II |
| 10 |
NC_000022.11 |
22 |
41579086 |
+ |
PMM1 |
NNNAGGGCCTAAATCCAGAA |
NGG |
2 |
0.1832 |
Tier II |
| 11 |
NC_000016.10 |
16 |
85903947 |
+ |
IRF8 |
NNNTGGTCCTAACTCCAGAA |
NAG |
2 |
0.1481 |
Tier II |
| 12 |
NC_000018.10 |
18 |
4147976 |
+ |
DLGAP1 |
NNNTGGGCCTCACTCCAGAA |
NAG |
2 |
0.1037 |
Tier II |
| 13 |
NC_000020.11 |
20 |
57222554 |
- |
BMP7 |
NNNTGGGCCTCACTCCAGAA |
NCG |
2 |
0.0429 |
Tier II |
| 14 |
NC_000005.10 |
5 |
59166696 |
- |
PDE4D |
NNNTGGGGCTAACTCCAGAA |
NTG |
2 |
0.025 |
Tier II |
| 15 |
NC_000011.10 |
11 |
124188131 |
+ |
OR10D3 |
NNNTGGGCTTAACTCCAGAA |
NGT |
2 |
0.0141 |
Tier II |
| 16 |
NC_000014.9 |
14 |
21726746 |
+ |
TRA |
NNNTAGCCCTAACTCCAGAA |
NGG |
2 |
0.5958 |
Tier III |
| 17 |
NC_000010.11 |
10 |
86546285 |
+ |
LOC105378407 |
NNNTGGGAATAACTCCAGAA |
NGG |
2 |
0.5571 |
Tier III |
| 18 |
NC_000010.11 |
10 |
86546285 |
+ |
LOC105378408 |
NNNTGGGAATAACTCCAGAA |
NGG |
2 |
0.5571 |
Tier III |
| 19 |
NC_000023.11 |
X |
73538131 |
- |
MAP2K4P1 |
NNNTGGGACTGACTCCAGAA |
NGG |
2 |
0.4225 |
Tier III |
| 20 |
NC_000001.11 |
1 |
146429466 |
+ |
LOC105371235 |
NNNTGGGCCTCACACCAGAA |
NGG |
2 |
0.2476 |
Tier III |
| 21 |
NC_000001.11 |
1 |
149143668 |
- |
LOC102723348 |
NNNTGGGCCTCACACCAGAA |
NGG |
2 |
0.2476 |
Tier III |
| 22 |
NC_000002.12 |
2 |
5643620 |
+ |
LINC01248 |
NNNTGGGCCTCACACCAGAA |
NGG |
2 |
0.2476 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
101552515 |
+ |
Taf1 |
NNNTGGTCCTAACTCCAAAA |
NGG |
2 |
0.3956 |
Tier I |
| 2 |
NC_000068.7 |
2 |
28612993 |
+ |
Gfi1b |
NNNTGGGCCTAACTCCAGAG |
NAG |
2 |
0.1983 |
Tier II |
| 3 |
NC_000071.6 |
5 |
38807105 |
- |
Clnk |
NNNAGGGCCTAACTCCAGGA |
NGG |
2 |
0.1786 |
Tier II |
| 4 |
NC_000085.6 |
19 |
50168416 |
- |
Sorcs1 |
NNNTGGGCATAACTCCTGAA |
NGG |
2 |
0.1143 |
Tier II |
| 5 |
NC_000072.6 |
6 |
90726890 |
+ |
Iqsec1 |
NNNTGGGCCCAACTCCAGCA |
NGG |
2 |
0.1103 |
Tier II |
| 6 |
NC_000081.6 |
15 |
86429659 |
+ |
Tbc1d22a |
NNNTGGGCCTAAGTCCAGAA |
NAG |
2 |
0.0354 |
Tier II |
| 7 |
NC_000083.6 |
17 |
11553923 |
+ |
Prkn |
NNNTGGGCTTAACTCCAGAA |
NGT |
2 |
0.0141 |
Tier II |
| 8 |
NC_000070.6 |
4 |
81475303 |
- |
Gm11765 |
NNNTGGGCCTAAATCCAGAA |
NGT |
2 |
0.0062 |
Tier III |
Other clones with same target sequence:
(none)
