Construct: sgRNA BRDN0001148186
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTTGGTGAACCAGTCGACAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ATP23 (91419)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77437
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
57951855 |
- |
ATP23 |
NNNGGTGAACCAGTCGACAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
191994014 |
- |
TMEFF2 |
NNNGGTGAACAAGTCAACAT |
NGG |
2 |
0.75 |
Tier II |
| 3 |
NC_000006.12 |
6 |
146635826 |
+ |
ADGB |
NNNGGTTAGCCAGTCGACAT |
NGG |
2 |
0.381 |
Tier II |
| 4 |
NC_000001.11 |
1 |
5906630 |
+ |
NPHP4 |
NNNGGTGAACCAGCCGACTT |
NGG |
2 |
0.1538 |
Tier II |
| 5 |
NC_000008.11 |
8 |
118409435 |
- |
SAMD12 |
NNNGGTGAAGCAGTTGACAT |
NGG |
2 |
0.0259 |
Tier II |
| 6 |
NC_000002.12 |
2 |
191994014 |
- |
CAVIN2-AS1 |
NNNGGTGAACAAGTCAACAT |
NGG |
2 |
0.75 |
Tier III |
| 7 |
NC_000008.11 |
8 |
118409435 |
- |
LOC105375724 |
NNNGGTGAAGCAGTTGACAT |
NGG |
2 |
0.0259 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
57071552 |
- |
Ablim1 |
NNNGGTGAACCAGTCTACAA |
NGG |
2 |
0.0 |
Tier II |
| 2 |
NC_000072.6 |
6 |
129408923 |
- |
Clec12b |
NNNGGTGAAGCAGTCTACAT |
NGG |
2 |
0.0 |
Tier II |
| 3 |
NC_000072.6 |
6 |
129408923 |
- |
Clec9a |
NNNGGTGAAGCAGTCTACAT |
NGG |
2 |
0.0 |
Tier II |
| 4 |
NC_000075.6 |
9 |
41547639 |
- |
Mir100hg |
NNNGGTGAACCACTTGACAT |
NGG |
2 |
0.0281 |
Tier III |
Other clones with same target sequence:
(none)
