Construct: sgRNA BRDN0001148187

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TACATGGCAGAAGAATATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIK3C2G (5288)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75739

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 18282755 + PIK3C2G NNNATGGCAGAAGAATATAG NGG 0 1.0 Tier I
2 NC_000020.11 20 32920184 + EFCAB8 NNNATGGCAGAAGAATATGT NGG 2 0.2625 Tier I
3 NC_000009.12 9 112418918 + HSDL2 NNNATGGCAGAAGAATTTAA NGG 2 0.125 Tier I
4 NC_000015.10 15 37033697 + MEIS2 NNNATGACAGAAAAATATAG NGG 2 0.9231 Tier II
5 NC_000004.12 4 149653581 - IQCM NNNATTGCAGAAGAAAATAG NGG 2 0.6061 Tier II
6 NC_000001.11 1 185752644 - HMCN1 NNNATGGGAAAAGAATATAG NGG 2 0.6 Tier II
7 NC_000002.12 2 24277568 + ITSN2 NNNAAAGCAGAAGAATATAG NGG 2 0.5 Tier II
8 NC_000005.10 5 119460688 + HSD17B4 NNNATGCCAGAGGAATATAG NGG 2 0.4965 Tier II
9 NC_000001.11 1 145800401 - RNF115 NNNATTGCAGAAGAATATAT NGG 2 0.4667 Tier II
10 NC_000014.9 14 35762882 - RALGAPA1 NNNATGGCAGAAGGATATAA NGG 2 0.4375 Tier II
11 NC_000001.11 1 76331032 + ST6GALNAC3 NNNATGGCAGAAGAGCATAG NGG 2 0.4333 Tier II
12 NC_000011.10 11 8780476 + DENND2B NNNATGGCAAAAGAATACAG NGG 2 0.4 Tier II
13 NC_000001.11 1 108953265 - CLCC1 NNNATGCCAGAAGAATATAC NGG 2 0.2946 Tier II
14 NC_000007.14 7 18562696 - HDAC9 NNNATGGAAGAACAATATAG NGG 2 0.2737 Tier II
15 NC_000001.11 1 36447441 + OSCP1 NNNATGGCAGCAGAGTATAG NGG 2 0.26 Tier II
16 NC_000013.11 13 96479951 - HS6ST3 NNNATGGGAGCAGAATATAG NGG 2 0.2571 Tier II
17 NC_000006.12 6 47517184 - CD2AP NNNATGGCAGAAGAAGAAAG NGG 2 0.2308 Tier II
18 NC_000011.10 11 86833066 + PRSS23 NNNATGGCTGAATAATATAG NGG 2 0.18 Tier II
19 NC_000002.12 2 225450500 + NYAP2 NNNAAGGCAGAAGAAGATAG NGG 2 0.1731 Tier II
20 NC_000004.12 4 154244119 - DCHS2 NNNATGGCAGAAGAATGTAC NGG 2 0.0756 Tier II
21 NC_000015.10 15 33469866 + RYR3 NNNATGGCAGAAGAAAATAG NGA 2 0.0631 Tier II
22 NC_000001.11 1 91707280 - TGFBR3 NNNATGGCAGAAGAGTATAG NTG 2 0.0253 Tier II
23 NC_000007.14 7 123565180 + NDUFA5 NNNAAGGCAGAAGAATATAG NTG 2 0.0195 Tier II
24 NC_000004.12 4 150176027 + DCLK2 NNNATGGCAGAAGAAGATAG NTG 2 0.0135 Tier II
25 NC_000007.14 7 68031065 + LOC102723427 NNNATTGCAGAAAAATATAG NGG 2 0.6154 Tier III
26 NC_000002.12 2 106476045 - LOC107985931 NNNTTGGCAGAGGAATATAG NGG 2 0.4596 Tier III
27 NC_000014.9 14 35762882 - SNORA101B NNNATGGCAGAAGGATATAA NGG 2 0.4375 Tier III
28 NC_000011.10 11 8780476 + DENND2B-AS1 NNNATGGCAAAAGAATACAG NGG 2 0.4 Tier III
29 NC_000006.12 6 167693465 - LINC02487 NNNATGGCAGAAGAAGATAA NGG 2 0.3245 Tier III
30 NC_000001.11 1 36447441 + LOC107984940 NNNATGGCAGCAGAGTATAG NGG 2 0.26 Tier III
31 NC_000011.10 11 3391350 + OR7E12P NNNATGGCTGAACAATATAG NGG 2 0.2526 Tier III
32 NC_000011.10 11 3391350 + TSSC2 NNNATGGCTGAACAATATAG NGG 2 0.2526 Tier III
33 NC_000011.10 11 17052448 - OR7E14P NNNATGGCTGAATAATATAG NGG 2 0.18 Tier III
34 NC_000011.10 11 86833066 + OR7E13P NNNATGGCTGAATAATATAG NGG 2 0.18 Tier III
35 NC_000015.10 15 39032487 + LOC105370777 NNNATGTCAGAAGAATATAG NAG 2 0.1481 Tier III
36 NC_000004.12 4 154244119 - LOC101927947 NNNATGGCAGAAGAATGTAC NGG 2 0.0756 Tier III
37 NC_000017.11 17 52431584 - LINC01982 NNNATGCCAGAAGAATATAG NGA 2 0.0477 Tier III
38 NC_000011.10 11 116638851 - LOC107984372 NNNATGGCAGAAGAATAAAG NGA 2 0.0463 Tier III
39 NC_000013.11 13 44159496 + SMIM2 NNNATGGCAGAAGAATTTAG NAG 2 0.0346 Tier III
40 NC_000023.11 X 113172630 - LOC101928437 NNNATGGCAGGAGAATATAG NTG 2 0.0253 Tier III
41 NC_000007.14 7 19299901 - LOC107986773 NNNATGGCAGAAGTATATAG NTG 2 0.0208 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 127062644 + Gm382 NNNATGGCAGAAGACTATGG NGG 2 0.1023 Tier I
2 NC_000070.6 4 59597315 + Hsdl2 NNNATGGCTGAAGAATTTAG NGG 2 0.08 Tier I
3 NC_000079.6 13 76559188 - Mctp1 NNNATGGCAGAAGAATATAG NGG 0 1.0 Tier II
4 NC_000081.6 15 6449910 + C9 NNNATGGTAGAAGAAAATAG NGG 2 0.7955 Tier II
5 NC_000072.6 6 80125406 - Lrrtm4 NNNATGGCATAAAAATATAG NGG 2 0.75 Tier II
6 NC_000078.6 12 99762106 + Efcab11 NNNATGACAGGAGAATATAG NGG 2 0.65 Tier II
7 NC_000086.7 X 71150217 - Mamld1 NNNAGGGCAGAAAAATATAG NGG 2 0.5908 Tier II
8 NC_000084.6 18 72227846 - Dcc NNNATGTCAGAAGAAAATAG NGG 2 0.5195 Tier II
9 NC_000086.7 X 144656577 + Trpc5 NNNATGGCAAAAGTATATAG NGG 2 0.4978 Tier II
10 NC_000073.6 7 19636573 - Clptm1 NNNATCCCAGAAGAATATAG NGG 2 0.4687 Tier II
11 NC_000076.6 10 75582326 + Ggt1 NNNATGGCAGAAAGATATAG NGG 2 0.4308 Tier II
12 NC_000067.6 1 106583688 + Bcl2 NNNATGGCAGAAGAATATTT NGG 2 0.3769 Tier II
13 NC_000077.6 11 9059463 - Gm11992 NNNATGGCAGAAGAATAAGG NGG 2 0.25 Tier II
14 NC_000075.6 9 92270977 + Plscr1 NNNATGCCAGAAGAATAGAG NGG 2 0.2292 Tier II
15 NC_000082.6 16 24579170 + Lpp NNNATGGCAGAAGTATATAG NAG 2 0.1383 Tier II
16 NC_000067.6 1 116501884 + Cntnap5a NNNATGGCAGAAGGCTATAG NGG 2 0.1273 Tier II
17 NC_000072.6 6 144097202 - Sox5 NNNATGGCAGAAAAATTTAG NGG 2 0.1231 Tier II
18 NC_000068.7 2 175113815 + Gm14393 NNNATTGCAGAAGAATCTAG NGG 2 0.1176 Tier II
19 NC_000076.6 10 4022065 + Mthfd1l NNNATGGCAGAAGCATATTG NGG 2 0.1154 Tier II
20 NC_000068.7 2 175669964 - Gm4724 NNNATTGCAGAAGAATTTAG NGG 2 0.0889 Tier II
21 NC_000068.7 2 31644228 + Prdm12 NNNAGGGCAGAAGAATATAG NGA 2 0.0444 Tier II
22 NC_000081.6 15 71640034 - Fam135b NNNATGGCAGAAAAATATAG NGC 2 0.0205 Tier II
23 NC_000067.6 1 178193823 - Desi2 NNNATGGCAGAAGACTATAG NTG 2 0.0106 Tier II
24 NC_000073.6 7 124196150 + Hs3st4 NNNATGGCAGAAGAATATAG NCT 2 0.0 Tier II
25 NC_000070.6 4 143460291 - Gm13042 NNNATGACAGAAGAGTATAG NGG 2 0.65 Tier III
26 NC_000075.6 9 106580300 + Gm39420 NNNATGACAGAAGAATACAG NGG 2 0.4286 Tier III
27 NC_000075.6 9 106580300 + Gm39421 NNNATGACAGAAGAATACAG NGG 2 0.4286 Tier III
28 NC_000067.6 1 28831894 + Gm45942 NNNATGGGAGAAGAATAAAG NGG 2 0.4286 Tier III
29 NC_000076.6 10 92173333 + Gm20757 NNNATGGGTGAAGAATATAG NGG 2 0.3857 Tier III
30 NC_000070.6 4 125888113 + 2610028E06Rik NNNATGGCAGAAGTATATTG NGG 2 0.2872 Tier III
31 NC_000070.6 4 28281928 + Gm52725 NNNATGTCAGAAGGATATAG NGG 2 0.2667 Tier III
32 NC_000067.6 1 180789153 - 2900024J01Rik NNNATGGCAGAAGCATACAG NGG 2 0.0918 Tier III
33 NC_000068.7 2 174991905 + Gm52571 NNNATTGCAGAAGAATTTAG NGG 2 0.0889 Tier III
34 NC_000068.7 2 177290674 - Gm14412 NNNATTGCAGAAGAATTTAG NGG 2 0.0889 Tier III
35 NC_000068.7 2 177342285 - Gm14412 NNNATTGCAGAAGAATTTAG NGG 2 0.0889 Tier III
36 NC_000075.6 9 25212800 - Gm29642 NNNATGGCAGATGAATATAG NAG 2 0.0864 Tier III
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Other clones with same target sequence:

(none)