Construct: sgRNA BRDN0001148188
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTCCAGACCAGGGACAACCA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- VRK3 (51231)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000019.10 | 19 | 50007589 | - | VRK3 | NNNCAGACCAGGGACAACCA | NGG | 0 | 1.0 | Tier I |
2 | NC_000003.12 | 3 | 79745188 | - | ROBO1 | NNNCAGAGCAGGGACAACCA | NGG | 1 | 0.6429 | Tier II |
3 | NC_000004.12 | 4 | 10491468 | + | CLNK | NNNAAGAACAGGGACAACCA | NGG | 2 | 0.5474 | Tier II |
4 | NC_000018.10 | 18 | 49598783 | - | LIPG | NNNCAGATCAGGGACAACCT | NGG | 2 | 0.525 | Tier II |
5 | NC_000010.11 | 10 | 590228 | + | DIP2C | NNNCAGGCCAGGGGCAACCA | NGG | 2 | 0.3294 | Tier II |
6 | NC_000004.12 | 4 | 40966921 | + | APBB2 | NNNCAGGCCAGGGACAACCA | NAG | 2 | 0.183 | Tier II |
7 | NC_000014.9 | 14 | 64729167 | - | PLEKHG3 | NNNGAGACCAGGGACATCCA | NGG | 2 | 0.0667 | Tier II |
8 | NC_000015.10 | 15 | 47718914 | - | SEMA6D | NNNCAGACCAGGGACAAACA | NCG | 2 | 0.0577 | Tier II |
9 | NC_000020.11 | 20 | 3121207 | - | UBOX5 | NNNCAGACCAGGGACAACCT | NGA | 2 | 0.0417 | Tier II |
10 | NC_000005.10 | 5 | 132329782 | + | SLC22A4 | NNNCAGGCCAGGGACAACCA | NTG | 2 | 0.0275 | Tier II |
11 | NC_000017.11 | 17 | 17734559 | + | RAI1 | NNNCAGACCAGGGCTAACCA | NGG | 2 | 0.0143 | Tier II |
12 | NC_000017.11 | 17 | 9389254 | + | STX8 | NNNCAGACCAGGCACTACCA | NGG | 2 | 0.0 | Tier II |
13 | NC_000020.11 | 20 | 57669739 | + | PMEPA1 | NNNCAGACCAGGGACCAGCA | NGG | 2 | 0.0 | Tier II |
14 | NC_000018.10 | 18 | 68732864 | + | CCDC102B | NNNCAGACCAGGGACCATCA | NGG | 2 | 0.0 | Tier II |
15 | NC_000002.12 | 2 | 73496271 | - | ALMS1 | NNNCAGACCAGGGACTGCCA | NGG | 2 | 0.0 | Tier II |
16 | NC_000002.12 | 2 | 10172299 | - | RRM2 | NNNCAGCCCAGGGACCACCA | NGG | 2 | 0.0 | Tier II |
17 | NC_000002.12 | 2 | 6633068 | + | LINC01246 | NNNCAAAACAGGGACAACCA | NGG | 2 | 0.65 | Tier III |
18 | NC_000004.12 | 4 | 10491468 | + | LOC105374482 | NNNAAGAACAGGGACAACCA | NGG | 2 | 0.5474 | Tier III |
19 | NC_000009.12 | 9 | 82676449 | + | LOC107987087 | NNNCAGACCTGGGACAAACA | NGG | 2 | 0.4751 | Tier III |
20 | NC_000012.12 | 12 | 131355826 | - | LINC02370 | NNNCAGACCAGTGACAACCA | NGG | 1 | 0.3846 | Tier III |
21 | NC_000003.12 | 3 | 59482381 | + | CFAP20DC-DT | NNNCAGACCAGAGAAAACCA | NGG | 2 | 0.2074 | Tier III |
22 | NC_000012.12 | 12 | 126160923 | + | LINC02359 | NNNCATACCAGGGACAACCC | NGG | 2 | 0.1515 | Tier III |
23 | NC_000012.12 | 12 | 126160923 | + | LOC107984447 | NNNCATACCAGGGACAACCC | NGG | 2 | 0.1515 | Tier III |
24 | NC_000020.11 | 20 | 3121207 | - | UBOX5-AS1 | NNNCAGACCAGGGACAACCT | NGA | 2 | 0.0417 | Tier III |
25 | NC_000020.11 | 20 | 63502745 | + | LOC105372725 | NNNCAGACCAGGGCCAGCCA | NGG | 2 | 0.0378 | Tier III |
26 | NC_000007.14 | 7 | 28987145 | + | CPVL-AS2 | NNNCAGACCAGGGAAAACGA | NGG | 2 | 0.0278 | Tier III |
27 | NC_000005.10 | 5 | 132329782 | + | MIR3936HG | NNNCAGGCCAGGGACAACCA | NTG | 2 | 0.0275 | Tier III |
28 | NC_000023.11 | X | 18057590 | + | LINC01456 | NNNCAGACCAGGGACAACCA | NGT | 1 | 0.0161 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000076.6 | 10 | 123127408 | - | Usp15 | NNNCAGAACTGGGACAACCA | NGG | 2 | 0.5735 | Tier II |
2 | NC_000085.6 | 19 | 8766870 | + | Nxf1 | NNNAAGAACAGGGACAACCA | NGG | 2 | 0.5474 | Tier II |
3 | NC_000071.6 | 5 | 75076842 | + | Gsx2 | NNNCAGACCAGGGACAAACA | NGG | 1 | 0.5385 | Tier II |
4 | NC_000070.6 | 4 | 63487063 | + | Whrn | NNNAAGACCAGGGACAAACA | NGG | 2 | 0.4534 | Tier II |
5 | NC_000072.6 | 6 | 84868586 | + | Exoc6b | NNNCATACCAGGGACAATCA | NGG | 2 | 0.4286 | Tier II |
6 | NC_000072.6 | 6 | 91389809 | - | Wnt7a | NNNCAGAACAGGGACAACCT | NGG | 2 | 0.39 | Tier II |
7 | NC_000082.6 | 16 | 18309428 | + | Tango2 | NNNCAGTCCAGGGACAACTA | NGG | 2 | 0.2019 | Tier II |
8 | NC_000067.6 | 1 | 93782489 | + | Atg4b | NNNCAGAACAGGTACAACCA | NGG | 2 | 0.195 | Tier II |
9 | NC_000072.6 | 6 | 91166106 | - | Hdac11 | NNNCAGACCAGTGGCAACCA | NGG | 2 | 0.1795 | Tier II |
10 | NC_000067.6 | 1 | 191617324 | + | Ints7 | NNNGAGACCCGGGACAACCA | NGG | 2 | 0.1667 | Tier II |
11 | NC_000070.6 | 4 | 132671884 | - | Eya3 | NNNCACACCAGGGACAGCCA | NGG | 2 | 0.1203 | Tier II |
12 | NC_000081.6 | 15 | 74777947 | - | Ly6g6g | NNNCAGACCAGGGACAGTCA | NGG | 2 | 0.1134 | Tier II |
13 | NC_000077.6 | 11 | 5129921 | - | Emid1 | NNNCAGACCAGGGTCAGCCA | NGG | 2 | 0.0941 | Tier II |
14 | NC_000074.6 | 8 | 109537572 | + | Pmfbp1 | NNNCAGAGCAGGGACATCCA | NGG | 2 | 0.0857 | Tier II |
15 | NC_000067.6 | 1 | 188731143 | - | Ush2a | NNNCAGAACAGGGATAACCA | NGG | 2 | 0.0433 | Tier II |
16 | NC_000072.6 | 6 | 113884045 | - | Atp2b2 | NNNCAGTCCAGGGACAACCA | NGA | 2 | 0.0304 | Tier II |
17 | NC_000072.6 | 6 | 116712234 | - | Tmem72 | NNNCAGAACAGGGACAACCA | NTG | 2 | 0.0253 | Tier II |
18 | NC_000074.6 | 8 | 121764921 | + | Jph3 | NNNCAGACCAGGGAGAACCC | NGG | 2 | 0.0114 | Tier II |
19 | NC_000069.6 | 3 | 19150693 | - | Armc1 | NNNCAGACCAGGGATATCCA | NGG | 2 | 0.0089 | Tier II |
20 | NC_000070.6 | 4 | 41952895 | - | Fam205a4 | NNNCAGACCAGGGACATCCA | NGT | 2 | 0.0022 | Tier II |
21 | NC_000070.6 | 4 | 42305109 | - | Fam205a3 | NNNCAGACCAGGGACATCCA | NGT | 2 | 0.0022 | Tier II |
22 | NC_000072.6 | 6 | 50339239 | - | Osbpl3 | NNNCAGACCAGGGACATCCA | NGT | 2 | 0.0022 | Tier II |
23 | NC_000076.6 | 10 | 60615854 | - | Cdh23 | NNNCAGACCAGGGACATCCA | NGT | 2 | 0.0022 | Tier II |
24 | NT_187054.1 | 4 | 72256 | + | LOC108168680 | NNNCAGACCAGGGACATCCA | NGT | 2 | 0.0022 | Tier II |
25 | NC_000078.6 | 12 | 108217550 | + | Ccdc85c | NNNCAGACCAGGGAGCACCA | NGG | 2 | 0.0 | Tier II |
26 | NC_000077.6 | 11 | 59332329 | - | Wnt9a | NNNCAGAGCAGGGACCACCA | NGG | 2 | 0.0 | Tier II |
27 | NC_000070.6 | 4 | 130551371 | + | Nkain1 | NNNGAGACCAGGGACCACCA | NGG | 2 | 0.0 | Tier II |
28 | NC_000067.6 | 1 | 42402345 | + | Gm36678 | NNNCAAACCAGGGACAACTA | NGG | 2 | 0.4615 | Tier III |
29 | NC_000080.6 | 14 | 31457934 | - | Gm35907 | NNNCAGACAAGTGACAACCA | NGG | 2 | 0.3297 | Tier III |
30 | NC_000075.6 | 9 | 32922125 | - | Gm27162 | NNNCAGACCAGGCACAACCT | NGG | 2 | 0.2526 | Tier III |
31 | NC_000071.6 | 5 | 52976894 | + | 5033403H07Rik | NNNCAGTCCAGGGACAGCCA | NGG | 2 | 0.0772 | Tier III |
32 | NC_000071.6 | 5 | 52976894 | + | Gm30301 | NNNCAGTCCAGGGACAGCCA | NGG | 2 | 0.0772 | Tier III |
33 | NC_000083.6 | 17 | 6816196 | - | Gm2885 | NNNCAGACCAGGAACAACCA | NTG | 2 | 0.036 | Tier III |
34 | NC_000075.6 | 9 | 96898413 | + | Gm51657 | NNNCAGCCCAGGGAGAACCA | NGG | 2 | 0.0219 | Tier III |
35 | NC_000070.6 | 4 | 42044078 | + | LOC108169009 | NNNCAGACCAGGGACATCCA | NGT | 2 | 0.0022 | Tier III |
36 | NC_000070.6 | 4 | 42724447 | + | 4930578G10Rik | NNNCAGACCAGGGACATCCA | NGT | 2 | 0.0022 | Tier III |
37 | NT_187053.1 | 4 | 131605 | - | 4933409K07Rik | NNNCAGACCAGGGACATCCA | NGT | 2 | 0.0022 | Tier III |
38 | NT_187054.1 | 4 | 72256 | + | Gm2192 | NNNCAGACCAGGGACATCCA | NGT | 2 | 0.0022 | Tier III |