Construct: sgRNA BRDN0001148188

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTCCAGACCAGGGACAACCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VRK3 (51231)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75552

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 50007589 - VRK3 NNNCAGACCAGGGACAACCA NGG 0 1.0 Tier I
2 NC_000003.12 3 79745188 - ROBO1 NNNCAGAGCAGGGACAACCA NGG 1 0.6429 Tier II
3 NC_000004.12 4 10491468 + CLNK NNNAAGAACAGGGACAACCA NGG 2 0.5474 Tier II
4 NC_000018.10 18 49598783 - LIPG NNNCAGATCAGGGACAACCT NGG 2 0.525 Tier II
5 NC_000010.11 10 590228 + DIP2C NNNCAGGCCAGGGGCAACCA NGG 2 0.3294 Tier II
6 NC_000004.12 4 40966921 + APBB2 NNNCAGGCCAGGGACAACCA NAG 2 0.183 Tier II
7 NC_000014.9 14 64729167 - PLEKHG3 NNNGAGACCAGGGACATCCA NGG 2 0.0667 Tier II
8 NC_000015.10 15 47718914 - SEMA6D NNNCAGACCAGGGACAAACA NCG 2 0.0577 Tier II
9 NC_000020.11 20 3121207 - UBOX5 NNNCAGACCAGGGACAACCT NGA 2 0.0417 Tier II
10 NC_000005.10 5 132329782 + SLC22A4 NNNCAGGCCAGGGACAACCA NTG 2 0.0275 Tier II
11 NC_000017.11 17 17734559 + RAI1 NNNCAGACCAGGGCTAACCA NGG 2 0.0143 Tier II
12 NC_000017.11 17 9389254 + STX8 NNNCAGACCAGGCACTACCA NGG 2 0.0 Tier II
13 NC_000020.11 20 57669739 + PMEPA1 NNNCAGACCAGGGACCAGCA NGG 2 0.0 Tier II
14 NC_000018.10 18 68732864 + CCDC102B NNNCAGACCAGGGACCATCA NGG 2 0.0 Tier II
15 NC_000002.12 2 73496271 - ALMS1 NNNCAGACCAGGGACTGCCA NGG 2 0.0 Tier II
16 NC_000002.12 2 10172299 - RRM2 NNNCAGCCCAGGGACCACCA NGG 2 0.0 Tier II
17 NC_000002.12 2 6633068 + LINC01246 NNNCAAAACAGGGACAACCA NGG 2 0.65 Tier III
18 NC_000004.12 4 10491468 + LOC105374482 NNNAAGAACAGGGACAACCA NGG 2 0.5474 Tier III
19 NC_000009.12 9 82676449 + LOC107987087 NNNCAGACCTGGGACAAACA NGG 2 0.4751 Tier III
20 NC_000012.12 12 131355826 - LINC02370 NNNCAGACCAGTGACAACCA NGG 1 0.3846 Tier III
21 NC_000003.12 3 59482381 + CFAP20DC-DT NNNCAGACCAGAGAAAACCA NGG 2 0.2074 Tier III
22 NC_000012.12 12 126160923 + LINC02359 NNNCATACCAGGGACAACCC NGG 2 0.1515 Tier III
23 NC_000012.12 12 126160923 + LOC107984447 NNNCATACCAGGGACAACCC NGG 2 0.1515 Tier III
24 NC_000020.11 20 3121207 - UBOX5-AS1 NNNCAGACCAGGGACAACCT NGA 2 0.0417 Tier III
25 NC_000020.11 20 63502745 + LOC105372725 NNNCAGACCAGGGCCAGCCA NGG 2 0.0378 Tier III
26 NC_000007.14 7 28987145 + CPVL-AS2 NNNCAGACCAGGGAAAACGA NGG 2 0.0278 Tier III
27 NC_000005.10 5 132329782 + MIR3936HG NNNCAGGCCAGGGACAACCA NTG 2 0.0275 Tier III
28 NC_000023.11 X 18057590 + LINC01456 NNNCAGACCAGGGACAACCA NGT 1 0.0161 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 123127408 - Usp15 NNNCAGAACTGGGACAACCA NGG 2 0.5735 Tier II
2 NC_000085.6 19 8766870 + Nxf1 NNNAAGAACAGGGACAACCA NGG 2 0.5474 Tier II
3 NC_000071.6 5 75076842 + Gsx2 NNNCAGACCAGGGACAAACA NGG 1 0.5385 Tier II
4 NC_000070.6 4 63487063 + Whrn NNNAAGACCAGGGACAAACA NGG 2 0.4534 Tier II
5 NC_000072.6 6 84868586 + Exoc6b NNNCATACCAGGGACAATCA NGG 2 0.4286 Tier II
6 NC_000072.6 6 91389809 - Wnt7a NNNCAGAACAGGGACAACCT NGG 2 0.39 Tier II
7 NC_000082.6 16 18309428 + Tango2 NNNCAGTCCAGGGACAACTA NGG 2 0.2019 Tier II
8 NC_000067.6 1 93782489 + Atg4b NNNCAGAACAGGTACAACCA NGG 2 0.195 Tier II
9 NC_000072.6 6 91166106 - Hdac11 NNNCAGACCAGTGGCAACCA NGG 2 0.1795 Tier II
10 NC_000067.6 1 191617324 + Ints7 NNNGAGACCCGGGACAACCA NGG 2 0.1667 Tier II
11 NC_000070.6 4 132671884 - Eya3 NNNCACACCAGGGACAGCCA NGG 2 0.1203 Tier II
12 NC_000081.6 15 74777947 - Ly6g6g NNNCAGACCAGGGACAGTCA NGG 2 0.1134 Tier II
13 NC_000077.6 11 5129921 - Emid1 NNNCAGACCAGGGTCAGCCA NGG 2 0.0941 Tier II
14 NC_000074.6 8 109537572 + Pmfbp1 NNNCAGAGCAGGGACATCCA NGG 2 0.0857 Tier II
15 NC_000067.6 1 188731143 - Ush2a NNNCAGAACAGGGATAACCA NGG 2 0.0433 Tier II
16 NC_000072.6 6 113884045 - Atp2b2 NNNCAGTCCAGGGACAACCA NGA 2 0.0304 Tier II
17 NC_000072.6 6 116712234 - Tmem72 NNNCAGAACAGGGACAACCA NTG 2 0.0253 Tier II
18 NC_000074.6 8 121764921 + Jph3 NNNCAGACCAGGGAGAACCC NGG 2 0.0114 Tier II
19 NC_000069.6 3 19150693 - Armc1 NNNCAGACCAGGGATATCCA NGG 2 0.0089 Tier II
20 NC_000070.6 4 41952895 - Fam205a4 NNNCAGACCAGGGACATCCA NGT 2 0.0022 Tier II
21 NC_000070.6 4 42305109 - Fam205a3 NNNCAGACCAGGGACATCCA NGT 2 0.0022 Tier II
22 NC_000072.6 6 50339239 - Osbpl3 NNNCAGACCAGGGACATCCA NGT 2 0.0022 Tier II
23 NC_000076.6 10 60615854 - Cdh23 NNNCAGACCAGGGACATCCA NGT 2 0.0022 Tier II
24 NT_187054.1 4 72256 + LOC108168680 NNNCAGACCAGGGACATCCA NGT 2 0.0022 Tier II
25 NC_000078.6 12 108217550 + Ccdc85c NNNCAGACCAGGGAGCACCA NGG 2 0.0 Tier II
26 NC_000077.6 11 59332329 - Wnt9a NNNCAGAGCAGGGACCACCA NGG 2 0.0 Tier II
27 NC_000070.6 4 130551371 + Nkain1 NNNGAGACCAGGGACCACCA NGG 2 0.0 Tier II
28 NC_000067.6 1 42402345 + Gm36678 NNNCAAACCAGGGACAACTA NGG 2 0.4615 Tier III
29 NC_000080.6 14 31457934 - Gm35907 NNNCAGACAAGTGACAACCA NGG 2 0.3297 Tier III
30 NC_000075.6 9 32922125 - Gm27162 NNNCAGACCAGGCACAACCT NGG 2 0.2526 Tier III
31 NC_000071.6 5 52976894 + 5033403H07Rik NNNCAGTCCAGGGACAGCCA NGG 2 0.0772 Tier III
32 NC_000071.6 5 52976894 + Gm30301 NNNCAGTCCAGGGACAGCCA NGG 2 0.0772 Tier III
33 NC_000083.6 17 6816196 - Gm2885 NNNCAGACCAGGAACAACCA NTG 2 0.036 Tier III
34 NC_000075.6 9 96898413 + Gm51657 NNNCAGCCCAGGGAGAACCA NGG 2 0.0219 Tier III
35 NC_000070.6 4 42044078 + LOC108169009 NNNCAGACCAGGGACATCCA NGT 2 0.0022 Tier III
36 NC_000070.6 4 42724447 + 4930578G10Rik NNNCAGACCAGGGACATCCA NGT 2 0.0022 Tier III
37 NT_187053.1 4 131605 - 4933409K07Rik NNNCAGACCAGGGACATCCA NGT 2 0.0022 Tier III
38 NT_187054.1 4 72256 + Gm2192 NNNCAGACCAGGGACATCCA NGT 2 0.0022 Tier III
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Other clones with same target sequence:

(none)