Construct: sgRNA BRDN0001148192
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CACACCCCAGAAAACGCTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAPK10 (5602)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75981
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
86103205 |
- |
MAPK10 |
NNNACCCCAGAAAACGCTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
17194825 |
+ |
MYO9B |
NNNACCCCAGAAAACCGTGG |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000001.11 |
1 |
196542976 |
+ |
KCNT2 |
NNNACTACAGAAAACGCTGG |
NGG |
2 |
0.6964 |
Tier II |
| 4 |
NC_000020.11 |
20 |
19324071 |
+ |
SLC24A3 |
NNNAGCCCAGAAAACGCTGC |
NGG |
2 |
0.2571 |
Tier II |
| 5 |
NC_000005.10 |
5 |
76584463 |
+ |
IQGAP2 |
NNNAGCCCACAAAACGCTGG |
NGG |
2 |
0.24 |
Tier II |
| 6 |
NC_000016.10 |
16 |
77915840 |
+ |
VAT1L |
NNNACCCCAGAACACACTGG |
NGG |
2 |
0.2105 |
Tier II |
| 7 |
NC_000007.14 |
7 |
34104386 |
+ |
BMPER |
NNNACCCCAGCATACGCTGG |
NGG |
2 |
0.12 |
Tier II |
| 8 |
NC_000009.12 |
9 |
32414592 |
- |
ACO1 |
NNNACCCCAGAAAATGCTGG |
NGG |
1 |
0.0667 |
Tier II |
| 9 |
NC_000010.11 |
10 |
103748031 |
- |
SH3PXD2A |
NNNACCACAGAAAATGCTGG |
NGG |
2 |
0.05 |
Tier II |
| 10 |
NC_000021.9 |
21 |
42279682 |
- |
ABCG1 |
NNNACCCCGGAAAAGGCTGG |
NGG |
2 |
0.0333 |
Tier II |
| 11 |
NC_000011.10 |
11 |
12235135 |
+ |
MICAL2 |
NNNACCCCAGAAAAGGATGG |
NGG |
2 |
0.0233 |
Tier II |
| 12 |
NC_000001.11 |
1 |
193187111 |
- |
CDC73 |
NNNACCCCAGAAAACGGGGG |
NGG |
2 |
0.0196 |
Tier II |
| 13 |
NC_000016.10 |
16 |
61825513 |
+ |
CDH8 |
NNNACCCCAGAAAACCCTGG |
NAG |
2 |
0.0 |
Tier II |
| 14 |
NC_000017.11 |
17 |
74992316 |
+ |
CDR2L |
NNNACCCCAGAAAACCCTGG |
NGT |
2 |
0.0 |
Tier II |
| 15 |
NC_000017.11 |
17 |
38756241 |
- |
PSMB3 |
NNNACCCCAGAACACTCTGG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000011.10 |
11 |
112466977 |
+ |
LOC107984389 |
NNNACCCCAGAAAACACTGG |
NGA |
2 |
0.0694 |
Tier III |
| 17 |
NC_000010.11 |
10 |
103748031 |
- |
SH3PXD2A-AS1 |
NNNACCACAGAAAATGCTGG |
NGG |
2 |
0.05 |
Tier III |
| 18 |
NC_000009.12 |
9 |
32738875 |
- |
LOC105376017 |
NNNACCCCAGAAAAAGCTGG |
NGA |
2 |
0.0154 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
102991888 |
- |
Mapk10 |
NNNACCCCAGAAAACACTGG |
NGG |
1 |
1.0 |
Tier I |
| 2 |
NC_000073.6 |
7 |
27321892 |
+ |
Ltbp4 |
NNNACCCCAGAAAACACTGA |
NGG |
2 |
0.9375 |
Tier II |
| 3 |
NC_000067.6 |
1 |
34131548 |
- |
Dst |
NNNACCCCAGGAAACGCTGG |
NGG |
1 |
0.65 |
Tier II |
| 4 |
NC_000083.6 |
17 |
29319709 |
+ |
Pi16 |
NNNAGCCCAGAAAACACTGG |
NGG |
2 |
0.6 |
Tier II |
| 5 |
NC_000073.6 |
7 |
45286764 |
+ |
Slc6a21 |
NNNAACCCAGAAAACGCTGG |
NTG |
2 |
0.0223 |
Tier II |
| 6 |
NC_000072.6 |
6 |
97288209 |
- |
Frmd4b |
NNNACCCCACAAAACCCTGG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000079.6 |
13 |
29950228 |
+ |
E2f3 |
NNNACCCCAGAAAACTCTTG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000067.6 |
1 |
34131548 |
- |
Gm51742 |
NNNACCCCAGGAAACGCTGG |
NGG |
1 |
0.65 |
Tier III |
| 9 |
NC_000067.6 |
1 |
48730914 |
+ |
Gm8354 |
NNNACCCAAGAAAACGATGG |
NGG |
2 |
0.3033 |
Tier III |
| 10 |
NC_000071.6 |
5 |
42116971 |
- |
Gm16223 |
NNNCCCCCAGAAAATGCTGG |
NGG |
2 |
0.0235 |
Tier III |
Other clones with same target sequence:
(none)
