Construct: sgRNA BRDN0001148194
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TATACAGCTGCGTAAAGTGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CHUK (1147)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77035
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
100205155 |
- |
CHUK |
NNNACAGCTGCGTAAAGTGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
40396779 |
- |
PRX |
NNNACAGCTGCTGAAAGTGT |
NGG |
2 |
0.1003 |
Tier I |
| 3 |
NC_000015.10 |
15 |
83798968 |
- |
ADAMTSL3 |
NNNACAGCTGCCTAAAATGT |
NGG |
2 |
0.4941 |
Tier II |
| 4 |
NC_000016.10 |
16 |
27855986 |
+ |
GSG1L |
NNNACAGCTGCCCAAAGTGT |
NGG |
2 |
0.418 |
Tier II |
| 5 |
NC_000011.10 |
11 |
20868979 |
+ |
NELL1 |
NNNACAGCTGAGTTAAGTGT |
NGG |
2 |
0.4 |
Tier II |
| 6 |
NC_000018.10 |
18 |
52529261 |
- |
DCC |
NNNACAGCTGCTTAAAATGT |
NGG |
2 |
0.359 |
Tier II |
| 7 |
NC_000005.10 |
5 |
31249861 |
- |
CDH6 |
NNNAAAGCTGCGTGAAGTGT |
NGG |
2 |
0.2667 |
Tier II |
| 8 |
NC_000010.11 |
10 |
75018541 |
+ |
KAT6B |
NNNACAGCTGCGGAAAGCGT |
NGG |
2 |
0.1118 |
Tier II |
| 9 |
NC_000022.11 |
22 |
20977237 |
- |
AIFM3 |
NNNACAGCTCCGTAAAGTGG |
NGG |
2 |
0.0706 |
Tier II |
| 10 |
NC_000013.11 |
13 |
66591280 |
- |
PCDH9 |
NNNACAGCTGCATAAAGTGT |
NTG |
2 |
0.0364 |
Tier II |
| 11 |
NC_000007.14 |
7 |
78406747 |
- |
MAGI2 |
NNNACAGCTGTGTAAAGTGT |
NTG |
2 |
0.012 |
Tier II |
| 12 |
NC_000021.9 |
21 |
26179634 |
+ |
APP-DT |
NNNTCAGCTGCCTAAAGTGT |
NGG |
2 |
0.3369 |
Tier III |
| 13 |
NC_000013.11 |
13 |
66591280 |
- |
LOC105370247 |
NNNACAGCTGCATAAAGTGT |
NTG |
2 |
0.0364 |
Tier III |
| 14 |
NC_000002.12 |
2 |
228567828 |
+ |
LINC01807 |
NNNACAGGTGCGTAAAGTGT |
NTG |
2 |
0.025 |
Tier III |
| 15 |
NC_000004.12 |
4 |
64979969 |
- |
LINC02232 |
NNNACAGCTGGGTAATGTGT |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
44087995 |
- |
Chuk |
NNNACAGCTGCGGAAAGTAT |
NGG |
2 |
0.1863 |
Tier I |
| 2 |
NC_000067.6 |
1 |
177906456 |
+ |
Catspere1 |
NNNACAGCAGAGTAAAGTGT |
NGG |
2 |
0.6964 |
Tier II |
| 3 |
NC_000067.6 |
1 |
178121891 |
+ |
Catspere2 |
NNNACAGCAGAGTAAAGTGT |
NGG |
2 |
0.6964 |
Tier II |
| 4 |
NC_000070.6 |
4 |
85300754 |
- |
Sh3gl2 |
NNNATAGCTTCGTAAAGTGT |
NGG |
2 |
0.517 |
Tier II |
| 5 |
NC_000074.6 |
8 |
22481258 |
+ |
Slc20a2 |
NNNACAGCTGAGTAAAGTTT |
NGG |
2 |
0.5 |
Tier II |
| 6 |
NC_000074.6 |
8 |
125494858 |
- |
Sipa1l2 |
NNNACAGCAGCCTAAAGTGT |
NGG |
2 |
0.4916 |
Tier II |
| 7 |
NC_000071.6 |
5 |
111245685 |
- |
Ttc28 |
NNNACACCTGTGTAAAGTGT |
NGG |
2 |
0.2115 |
Tier II |
| 8 |
NC_000082.6 |
16 |
91725019 |
- |
Cryzl1 |
NNNCAAGCTGCGTAAAGTGT |
NGG |
2 |
0.2017 |
Tier II |
| 9 |
NC_000070.6 |
4 |
102565987 |
+ |
Pde4b |
NNNCCAGCTGCGTTAAGTGT |
NGG |
2 |
0.1882 |
Tier II |
| 10 |
NC_000068.7 |
2 |
73824854 |
+ |
Atf2 |
NNNACAGCTGGGTAAATTGT |
NGG |
2 |
0.0625 |
Tier II |
| 11 |
NC_000083.6 |
17 |
57150356 |
- |
Gm33566 |
NNNACAGCTGAGTTAAGTGT |
NGG |
2 |
0.4 |
Tier III |
| 12 |
NC_000082.6 |
16 |
91725019 |
- |
Gm46543 |
NNNCAAGCTGCGTAAAGTGT |
NGG |
2 |
0.2017 |
Tier III |
| 13 |
NC_000070.6 |
4 |
102565987 |
+ |
Gm52667 |
NNNCCAGCTGCGTTAAGTGT |
NGG |
2 |
0.1882 |
Tier III |
| 14 |
NC_000079.6 |
13 |
13436035 |
+ |
Gm7426 |
NNNTCAGCTGGGTAAAGTGT |
NGG |
2 |
0.1591 |
Tier III |
Other clones with same target sequence:
(none)
