Construct: sgRNA BRDN0001148197
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTTGACCGAGTGAAACTCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKCA (5578)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75924
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
66732776 |
+ |
PRKCA |
NNNTGACCGAGTGAAACTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
41711097 |
- |
LRFN5 |
NNNTGACAGAGAGAAACTCA |
NGG |
2 |
0.52 |
Tier II |
| 3 |
NC_000001.11 |
1 |
218329412 |
+ |
RRP15 |
NNNTGACAGAGTGAAAATCA |
NGG |
2 |
0.3033 |
Tier II |
| 4 |
NC_000013.11 |
13 |
99887745 |
+ |
CLYBL |
NNNTGACAGAGTGAAACCCA |
NGG |
2 |
0.2786 |
Tier II |
| 5 |
NC_000021.9 |
21 |
43931282 |
- |
AGPAT3 |
NNNTGACCGAGCGAAACTCC |
NGG |
2 |
0.2153 |
Tier II |
| 6 |
NC_000016.10 |
16 |
11955346 |
+ |
NPIPB2 |
NNNTGACCGAGTGAAACTCT |
NTG |
2 |
0.0234 |
Tier II |
| 7 |
NC_000015.10 |
15 |
82393266 |
- |
UBE2Q2P2 |
NNNTGAATGAGTGAAACTCA |
NGG |
2 |
0.6563 |
Tier III |
| 8 |
NC_000013.11 |
13 |
99887745 |
+ |
CLYBL-AS3 |
NNNTGACAGAGTGAAACCCA |
NGG |
2 |
0.2786 |
Tier III |
| 9 |
NC_000003.12 |
3 |
55338675 |
+ |
LOC124906243 |
NNNTTACCGAGTAAAACTCA |
NGG |
2 |
0.2769 |
Tier III |
| 10 |
NC_000012.12 |
12 |
116676113 |
- |
LINC03088 |
NNNTGACCGAGTGAAATTGA |
NGG |
2 |
0.0583 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
31407813 |
+ |
Prkg1 |
NNNTGAACAAGTGAAACTCA |
NGG |
2 |
0.4821 |
Tier II |
| 2 |
NC_000070.6 |
4 |
11566656 |
- |
Rad54b |
NNNTGAAGGAGTGAAACTCA |
NGG |
2 |
0.4821 |
Tier II |
| 3 |
NC_000079.6 |
13 |
96889359 |
- |
Ankrd31 |
NNNTGACCAAGTGAAACACA |
NGG |
2 |
0.4286 |
Tier II |
| 4 |
NC_000078.6 |
12 |
84788866 |
- |
Ltbp2 |
NNNTGACCAAGGGAAACTCA |
NGG |
2 |
0.3214 |
Tier II |
| 5 |
NC_000081.6 |
15 |
41706537 |
+ |
Oxr1 |
NNNTGCCCGAGTGAAACCCA |
NGG |
2 |
0.1948 |
Tier II |
| 6 |
NC_000083.6 |
17 |
48121058 |
- |
9830107B12Rik |
NNNTGACAGAGTGAAACTCA |
NTG |
2 |
0.0253 |
Tier II |
| 7 |
NC_000069.6 |
3 |
95997903 |
+ |
Plekho1 |
NNNTCACCGAGTGAACCTCA |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000082.6 |
16 |
33580300 |
- |
Slc12a8 |
NNNTGACAGAGTGAATCTCA |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
