Construct: sgRNA BRDN0001148201

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CAAGAAGCTAACTCGACCAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CPNE3 (8895)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77555

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 86531183 + CPNE3 NNNGAAGCTAACTCGACCAC NGG 0 1.0 Tier I
2 NC_000004.12 4 156793041 + PDGFC NNNGAAGCAAACTCAACCAC NGG 2 0.8739 Tier II
3 NC_000005.10 5 177013480 - UIMC1 NNNGAAGCTAAATTGACCAC NGG 2 0.5238 Tier II
4 NC_000011.10 11 131524029 - NTM NNNGAAGCTAACTAGACCAA NGG 2 0.175 Tier II
5 NC_000012.12 12 94591890 + TMCC3 NNNGAAGCTAACTGAACCAC NGG 2 0.0 Tier II
6 NC_000001.11 1 229139529 - LINC02815 NNNGATGCTAACGCGACCAC NGG 2 0.1863 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 19552291 - Cpne3 NNNGAAGCTAACTCGACCAC NGG 0 1.0 Tier I
2 NC_000086.7 X 153276321 + Klf8 NNNTTAGCTAACTCGACCAC NGG 2 0.1322 Tier II
3 NC_000077.6 11 48830441 - Trim7 NNNGAAGCTAACTAGGCCAC NGG 2 0.0673 Tier II
4 NC_000073.6 7 133666941 - Edrf1 NNNGAAGGTAACTGGACCAC NGG 2 0.0 Tier II
5 NC_000086.7 X 13757768 - Cask NNNGTAGCTAACTGGACCAC NGG 2 0.0 Tier II
6 NC_000076.6 10 18831802 - 4930444F02Rik NNNGAAGCTCACTCGGCCAC NGG 2 0.0641 Tier III
7 NC_000076.6 10 18831802 - Gm32926 NNNGAAGCTCACTCGGCCAC NGG 2 0.0641 Tier III
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Other clones with same target sequence:

(none)