Construct: sgRNA BRDN0001148201
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAAGAAGCTAACTCGACCAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CPNE3 (8895)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77555
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
86531183 |
+ |
CPNE3 |
NNNGAAGCTAACTCGACCAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
156793041 |
+ |
PDGFC |
NNNGAAGCAAACTCAACCAC |
NGG |
2 |
0.8739 |
Tier II |
| 3 |
NC_000005.10 |
5 |
177013480 |
- |
UIMC1 |
NNNGAAGCTAAATTGACCAC |
NGG |
2 |
0.5238 |
Tier II |
| 4 |
NC_000011.10 |
11 |
131524029 |
- |
NTM |
NNNGAAGCTAACTAGACCAA |
NGG |
2 |
0.175 |
Tier II |
| 5 |
NC_000012.12 |
12 |
94591890 |
+ |
TMCC3 |
NNNGAAGCTAACTGAACCAC |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000001.11 |
1 |
229139529 |
- |
LINC02815 |
NNNGATGCTAACGCGACCAC |
NGG |
2 |
0.1863 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
19552291 |
- |
Cpne3 |
NNNGAAGCTAACTCGACCAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000086.7 |
X |
153276321 |
+ |
Klf8 |
NNNTTAGCTAACTCGACCAC |
NGG |
2 |
0.1322 |
Tier II |
| 3 |
NC_000077.6 |
11 |
48830441 |
- |
Trim7 |
NNNGAAGCTAACTAGGCCAC |
NGG |
2 |
0.0673 |
Tier II |
| 4 |
NC_000073.6 |
7 |
133666941 |
- |
Edrf1 |
NNNGAAGGTAACTGGACCAC |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000086.7 |
X |
13757768 |
- |
Cask |
NNNGTAGCTAACTGGACCAC |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000076.6 |
10 |
18831802 |
- |
4930444F02Rik |
NNNGAAGCTCACTCGGCCAC |
NGG |
2 |
0.0641 |
Tier III |
| 7 |
NC_000076.6 |
10 |
18831802 |
- |
Gm32926 |
NNNGAAGCTCACTCGGCCAC |
NGG |
2 |
0.0641 |
Tier III |
Other clones with same target sequence:
(none)
