Construct: sgRNA BRDN0001148208
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGCACCTTGAACTTTGGCAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MKNK1 (8569)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75514
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
46580583 |
+ |
MKNK1 |
NNNACCTTGAACTTTGGCAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
41167918 |
- |
PLCG1 |
NNNACCTTGAAAGTTGGCAT |
NGG |
2 |
0.1863 |
Tier I |
| 3 |
NC_000002.12 |
2 |
135279707 |
- |
ZRANB3 |
NNNGGCTTGAACTTTGGCAT |
NGG |
2 |
0.375 |
Tier II |
| 4 |
NC_000017.11 |
17 |
8360104 |
- |
KRBA2 |
NNNACCTGGAACTTTGGCGT |
NGG |
2 |
0.275 |
Tier II |
| 5 |
NC_000012.12 |
12 |
1427753 |
- |
ERC1 |
NNNACCTTAACCTTTGGCAT |
NGG |
2 |
0.2571 |
Tier II |
| 6 |
NC_000004.12 |
4 |
94820253 |
- |
BMPR1B |
NNNACCATGAACTTTGGCAT |
NAG |
2 |
0.2269 |
Tier II |
| 7 |
NC_000011.10 |
11 |
68903509 |
- |
MRPL21 |
NNNCCCTTGAACTTTGGTAT |
NGG |
2 |
0.2269 |
Tier II |
| 8 |
NC_000001.11 |
1 |
160130641 |
- |
ATP1A2 |
NNNTCCTTGAACTCTGGCAT |
NGG |
2 |
0.1818 |
Tier II |
| 9 |
NC_000005.10 |
5 |
156413497 |
+ |
SGCD |
NNNACCTTGAACTTTGAGAT |
NGG |
2 |
0.1244 |
Tier II |
| 10 |
NC_000001.11 |
1 |
217577701 |
- |
GPATCH2 |
NNNACTTTGAACTTTGGCAT |
NGA |
2 |
0.0645 |
Tier II |
| 11 |
NC_000001.11 |
1 |
164709551 |
+ |
PBX1 |
NNNACCTTGAACTTTGTCAG |
NGG |
2 |
0.0441 |
Tier II |
| 12 |
NC_000009.12 |
9 |
89465857 |
- |
SEMA4D |
NNNACCTTGGACTTGGGCAT |
NGG |
2 |
0.0278 |
Tier II |
| 13 |
NC_000004.12 |
4 |
21789177 |
- |
KCNIP4 |
NNNACCTTGAACTTTGGAAT |
NTG |
2 |
0.021 |
Tier II |
| 14 |
NC_000002.12 |
2 |
26614798 |
- |
CIB4 |
NNNACCTTGAACTTGGGCAT |
NGC |
2 |
0.0011 |
Tier II |
| 15 |
NC_000009.12 |
9 |
11279607 |
- |
LOC105375974 |
NNNGGCTTGAACTTTGGCAT |
NGG |
2 |
0.375 |
Tier III |
| 16 |
NC_000012.12 |
12 |
115096397 |
+ |
LOC102723639 |
NNNATCTTGAACTTTGGAAT |
NGG |
2 |
0.3427 |
Tier III |
| 17 |
NC_000003.12 |
3 |
80664837 |
- |
LOC105377177 |
NNNGCCTTGAATTTTGGCAT |
NGG |
2 |
0.3365 |
Tier III |
| 18 |
NC_000017.11 |
17 |
8360104 |
- |
LOC100128288 |
NNNACCTGGAACTTTGGCGT |
NGG |
2 |
0.275 |
Tier III |
| 19 |
NC_000012.12 |
12 |
92020037 |
+ |
LINC01619 |
NNNACCTTGAAATTTGGCAT |
NAG |
2 |
0.1852 |
Tier III |
| 20 |
NC_000008.11 |
8 |
136570136 |
+ |
LINC02055 |
NNNACCTTGAGCTTTGGCAT |
NAG |
2 |
0.1685 |
Tier III |
| 21 |
NC_000003.12 |
3 |
163378587 |
- |
LOC105374188 |
NNNACCTTGAAGTTTGGCAT |
NGC |
2 |
0.0099 |
Tier III |
| 22 |
NC_000009.12 |
9 |
115226805 |
+ |
DELEC1 |
NNNACCTTGAACTTTGTCAT |
NGC |
2 |
0.0056 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
115860065 |
- |
Mknk1 |
NNNACCCTGGACTTTGGCAT |
NGG |
2 |
0.3819 |
Tier I |
| 2 |
NC_000068.7 |
2 |
160755807 |
- |
Plcg1 |
NNNACCTTGAAAGTTGGCAT |
NGG |
2 |
0.1863 |
Tier I |
| 3 |
NC_000084.6 |
18 |
78447228 |
+ |
Slc14a2 |
NNNACCTGGAACTATGGCAT |
NGG |
2 |
0.454 |
Tier II |
| 4 |
NC_000071.6 |
5 |
131196393 |
+ |
Galnt17 |
NNNACTTTGAAGTTTGGCAT |
NGG |
2 |
0.4127 |
Tier II |
| 5 |
NC_000076.6 |
10 |
106968333 |
- |
Acss3 |
NNNGCCTTGAACTTTGGCTT |
NGG |
2 |
0.3365 |
Tier II |
| 6 |
NC_000074.6 |
8 |
80504553 |
+ |
Gypa |
NNNACATTGAACTTTGGCAT |
NAG |
2 |
0.2407 |
Tier II |
| 7 |
NC_000071.6 |
5 |
25101332 |
+ |
Prkag2 |
NNNACCTTGATCTTTGGCAA |
NGG |
2 |
0.1731 |
Tier II |
| 8 |
NC_000074.6 |
8 |
33557072 |
+ |
Tex15 |
NNNACCTTGAACTTTGTCTT |
NGG |
2 |
0.1346 |
Tier II |
| 9 |
NC_000069.6 |
3 |
86564666 |
+ |
Lrba |
NNNACCTTGTACTTTGGCAT |
NGA |
2 |
0.0613 |
Tier II |
| 10 |
NC_000080.6 |
14 |
63787631 |
- |
Xkr6 |
NNNACCTTGAACTTGAGCAT |
NGG |
2 |
0.05 |
Tier II |
| 11 |
NC_000083.6 |
17 |
73642068 |
- |
Galnt14 |
NNNACCTTGAACTTGGGCCT |
NGG |
2 |
0.0103 |
Tier II |
| 12 |
NC_000078.6 |
12 |
118946355 |
- |
Abcb5 |
NNNACATTGAACTTTTGCAT |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000069.6 |
3 |
110067733 |
- |
Ntng1 |
NNNACCTTGAACTGTGGGAT |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000084.6 |
18 |
38895539 |
- |
Fgf1 |
NNNACCTTGAACTTTCTCAT |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000084.6 |
18 |
38895539 |
- |
Arhgap26 |
NNNACCTTGAACTTTCTCAT |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000084.6 |
18 |
38895539 |
- |
Gm5820 |
NNNACCTTGAACTTTCTCAT |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000072.6 |
6 |
61310691 |
+ |
Ccser1 |
NNNACCTTGAACTTTGGCAT |
NCT |
2 |
0.0 |
Tier II |
| 18 |
NC_000078.6 |
12 |
114708284 |
- |
Igh |
NNNAACTTGAACTTTGGAAT |
NGG |
2 |
0.3077 |
Tier III |
| 19 |
NC_000071.6 |
5 |
25101332 |
+ |
2900005J15Rik |
NNNACCTTGATCTTTGGCAA |
NGG |
2 |
0.1731 |
Tier III |
| 20 |
NC_000078.6 |
12 |
42969638 |
- |
Gm33340 |
NNNACCTTGAACTTTGGAAT |
NTG |
2 |
0.021 |
Tier III |
| 21 |
NC_000076.6 |
10 |
95420217 |
+ |
5730420D15Rik |
NNNACCTTGAACTTCGGCAT |
NGA |
2 |
0.0189 |
Tier III |
Other clones with same target sequence:
(none)
