Construct: sgRNA BRDN0001148211
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTGGGGAATAAGCCCCCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IGF1R (3480)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76690
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
98707991 |
+ |
IGF1R |
NNNGGGGAATAAGCCCCCAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
15125029 |
+ |
ILVBL |
NNNGGGGAATAAGCTCCCAG |
NGG |
2 |
0.051 |
Tier I |
| 3 |
NC_000004.12 |
4 |
21477786 |
- |
KCNIP4 |
NNNGGAGAATAAGTCCCCAA |
NGG |
2 |
0.7333 |
Tier II |
| 4 |
NC_000004.12 |
4 |
80863554 |
- |
CFAP299 |
NNNGGGGAATAAGACACCAA |
NGG |
2 |
0.35 |
Tier II |
| 5 |
NC_000001.11 |
1 |
164725260 |
+ |
PBX1 |
NNNGGTGCATAAGCCCCCAA |
NGG |
2 |
0.2857 |
Tier II |
| 6 |
NC_000022.11 |
22 |
30096152 |
- |
HORMAD2 |
NNNGGGGTATACGCCCCCAA |
NGG |
2 |
0.1128 |
Tier II |
| 7 |
NC_000011.10 |
11 |
108168212 |
+ |
NPAT |
NNNTGGGAATAAGCCCCCAA |
NGA |
2 |
0.0253 |
Tier II |
| 8 |
NC_000016.10 |
16 |
14834991 |
+ |
NOMO1 |
NNNGGGGAATAAGACCCCAA |
NGA |
2 |
0.0243 |
Tier II |
| 9 |
NC_000016.10 |
16 |
16233807 |
+ |
NOMO3 |
NNNGGGGAATAAGACCCCAA |
NGA |
2 |
0.0243 |
Tier II |
| 10 |
NC_000016.10 |
16 |
18560890 |
- |
NOMO2 |
NNNGGGGAATAAGACCCCAA |
NGA |
2 |
0.0243 |
Tier II |
| 11 |
NC_000001.11 |
1 |
108932677 |
- |
CLCC1 |
NNNGGGGAATATGCCCCCAA |
NGT |
2 |
0.0054 |
Tier II |
| 12 |
NC_000001.11 |
1 |
108932677 |
- |
GPSM2 |
NNNGGGGAATATGCCCCCAA |
NGT |
2 |
0.0054 |
Tier II |
| 13 |
NC_000019.10 |
19 |
15125029 |
+ |
ILVBL-AS1 |
NNNGGGGAATAAGCTCCCAG |
NGG |
2 |
0.051 |
Tier III |
| 14 |
NC_000007.14 |
7 |
53405638 |
- |
LOC105375282 |
NNNGTGGAATAAGCCCCCAA |
NTG |
2 |
0.0117 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
68004239 |
+ |
Igf1r |
NNNGGGGAACAAGCCCCCGA |
NGG |
2 |
0.2 |
Tier I |
| 2 |
NC_000074.6 |
8 |
32617254 |
+ |
Nrg1 |
NNNGGGGACTAAGCCACCAA |
NGG |
2 |
0.5714 |
Tier II |
| 3 |
NC_000080.6 |
14 |
117696675 |
- |
Gpc6 |
NNNGGGGAAAAAGCCCCTAA |
NGG |
2 |
0.551 |
Tier II |
| 4 |
NC_000083.6 |
17 |
30927030 |
- |
Glp1r |
NNNGGGAAAGAAGCCCCCAA |
NGG |
2 |
0.5 |
Tier II |
| 5 |
NC_000069.6 |
3 |
37382271 |
+ |
Fgf2 |
NNNGGAGAATAAGCCCTCAA |
NGG |
2 |
0.4667 |
Tier II |
| 6 |
NC_000082.6 |
16 |
14310779 |
+ |
Cep20 |
NNNGGGGAGGAAGCCCCCAA |
NGG |
2 |
0.3333 |
Tier II |
| 7 |
NC_000079.6 |
13 |
59120762 |
- |
Ntrk2 |
NNNGGGGAAAAAGCCCCCAA |
NAG |
2 |
0.2222 |
Tier II |
| 8 |
NC_000075.6 |
9 |
66432449 |
+ |
Herc1 |
NNNGGGCAATAAGCCTCCAA |
NGG |
2 |
0.2115 |
Tier II |
| 9 |
NC_000078.6 |
12 |
41713407 |
+ |
Immp2l |
NNNGGGCAATAAGCCTCCAA |
NGG |
2 |
0.2115 |
Tier II |
| 10 |
NC_000075.6 |
9 |
90197432 |
- |
Adamts7 |
NNNGGGGACTAAGACCCCAA |
NGG |
2 |
0.2 |
Tier II |
| 11 |
NC_000075.6 |
9 |
97499024 |
+ |
Clstn2 |
NNNGGGGAAGAAGCCCCCAA |
NGA |
2 |
0.0347 |
Tier II |
| 12 |
NC_000080.6 |
14 |
22608953 |
- |
Lrmda |
NNNGGGTAATAAGCCCCCAA |
NGT |
2 |
0.0092 |
Tier II |
| 13 |
NC_000076.6 |
10 |
89691441 |
+ |
1500026H17Rik |
NNNGAGGAATAAGCCCCCAA |
NGC |
2 |
0.0193 |
Tier III |
Other clones with same target sequence:
(none)
