Construct: sgRNA BRDN0001148212
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCACTGCCCGAGCTATCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- HIPK1 (204851)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76992
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
113940652 |
- |
HIPK1 |
NNNACTGCCCGAGCTATCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
17751922 |
- |
KCNC1 |
NNNCCTGCCTGAGCTATCAG |
NGG |
2 |
0.3322 |
Tier II |
| 3 |
NC_000002.12 |
2 |
202504198 |
+ |
BMPR2 |
NNNGCTGCCCGAGCTAGCAG |
NGG |
2 |
0.0735 |
Tier II |
| 4 |
NC_000005.10 |
5 |
113458130 |
+ |
MCC |
NNNGCTGCCCGAGCTAGCAG |
NGG |
2 |
0.0735 |
Tier II |
| 5 |
NC_000010.11 |
10 |
106927549 |
- |
SORCS1 |
NNNGCTGCCCGAGCTAGCAG |
NGG |
2 |
0.0735 |
Tier II |
| 6 |
NC_000013.11 |
13 |
24506419 |
- |
PARP4 |
NNNGCTGCCCGAGCTAGCAG |
NGG |
2 |
0.0735 |
Tier II |
| 7 |
NC_000001.11 |
1 |
230719257 |
- |
AGT |
NNNACTGCCCGAGGTATCAG |
NTG |
2 |
0.0 |
Tier II |
| 8 |
NC_000014.9 |
14 |
58834755 |
+ |
LINC01500 |
NNNACTGCCCCAGCCATCAG |
NGG |
2 |
0.1169 |
Tier III |
| 9 |
NC_000001.11 |
1 |
102659408 |
- |
LOC105378874 |
NNNGCTGCCCGAGCTAGCAG |
NGG |
2 |
0.0735 |
Tier III |
| 10 |
NC_000002.12 |
2 |
32886126 |
- |
LOC105374453 |
NNNGCTGCCCGAGCTAGCAG |
NGG |
2 |
0.0735 |
Tier III |
| 11 |
NC_000002.12 |
2 |
139410953 |
+ |
LOC105373645 |
NNNGCTGCCCGAGCTAGCAG |
NGG |
2 |
0.0735 |
Tier III |
| 12 |
NC_000003.12 |
3 |
36635166 |
+ |
NBPF21P |
NNNGCTGCCCGAGCTAGCAG |
NGG |
2 |
0.0735 |
Tier III |
| 13 |
NC_000004.12 |
4 |
79122774 |
+ |
LINC01088 |
NNNGCTGCCCGAGCTAGCAG |
NGG |
2 |
0.0735 |
Tier III |
| 14 |
NC_000005.10 |
5 |
113458130 |
+ |
LOC107986366 |
NNNGCTGCCCGAGCTAGCAG |
NGG |
2 |
0.0735 |
Tier III |
| 15 |
NC_000011.10 |
11 |
128238030 |
+ |
LINC02098 |
NNNGCTGCCCGAGCTAGCAG |
NGG |
2 |
0.0735 |
Tier III |
| 16 |
NC_000013.11 |
13 |
24506419 |
- |
LOC105370117 |
NNNGCTGCCCGAGCTAGCAG |
NGG |
2 |
0.0735 |
Tier III |
| 17 |
NC_000015.10 |
15 |
74479504 |
- |
UBL7-DT |
NNNGCTGCCCGAGCTAGCAG |
NGG |
2 |
0.0735 |
Tier III |
| 18 |
NT_187510.1 |
|
26353 |
+ |
LOC105379565 |
NNNGCTGCCCGAGCTAGCAG |
NGG |
2 |
0.0735 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
134522810 |
+ |
Clip2 |
NNNACTGCCCGAGCCCTCAG |
NGG |
2 |
0.0 |
Tier I |
| 2 |
NC_000078.6 |
12 |
91171395 |
+ |
Cep128 |
NNNGCTGCCAGAGCTATCAG |
NGG |
2 |
0.5417 |
Tier II |
| 3 |
NC_000082.6 |
16 |
56698520 |
+ |
Tfg |
NNNACTGCCCGAGCTAACAG |
NGG |
1 |
0.5333 |
Tier II |
| 4 |
NC_000075.6 |
9 |
28680855 |
- |
Opcml |
NNNAGTGCCCGAGCTAACAG |
NGG |
2 |
0.32 |
Tier II |
| 5 |
NC_000086.7 |
X |
18425088 |
- |
Dipk2b |
NNNACTGCCCTAGCTGTCAG |
NGG |
2 |
0.074 |
Tier II |
Other clones with same target sequence:
(none)
