Construct: sgRNA BRDN0001148217
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCCGTGGGAATGTCTAACGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- WNK1 (65125)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75624
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
754213 |
+ |
WNK1 |
NNNGTGGGAATGTCTAACGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
206577147 |
- |
ADAM23 |
NNNATGGGAATGTTTAACGA |
NGG |
2 |
0.66 |
Tier II |
| 3 |
NC_000008.11 |
8 |
90019644 |
+ |
DECR1 |
NNNGTGGGTATGTCTAAAGA |
NGG |
2 |
0.3231 |
Tier II |
| 4 |
NC_000007.14 |
7 |
151113462 |
- |
AGAP3 |
NNNGTGGGAATGCCTAAGGA |
NGG |
2 |
0.1053 |
Tier II |
| 5 |
NC_000012.12 |
12 |
29206971 |
+ |
FAR2 |
NNNGTGGGAATGTCTGAAGA |
NGG |
2 |
0.1036 |
Tier II |
| 6 |
NC_000001.11 |
1 |
172250629 |
+ |
DNM3 |
NNNGTGGGAATGTCAAAGGA |
NGG |
2 |
0.0772 |
Tier II |
| 7 |
NC_000014.9 |
14 |
69076198 |
+ |
DCAF5 |
NNNGTGGGAATGACTAACGA |
NCG |
2 |
0.0742 |
Tier II |
| 8 |
NC_000012.12 |
12 |
120831118 |
- |
SPPL3 |
NNNGTGGGAATGTCTAAGGA |
NCG |
2 |
0.0143 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
120036986 |
- |
Wnk1 |
NNNGTGGGAATGTCCAATGA |
NGG |
2 |
0.1753 |
Tier I |
| 2 |
NC_000076.6 |
10 |
73380984 |
- |
Pcdh15 |
NNNGTGAGAATGTCTAACTA |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000082.6 |
16 |
41934513 |
+ |
Lsamp |
NNNGTGGGCATGTCTAACGG |
NGG |
2 |
0.437 |
Tier II |
| 4 |
NC_000069.6 |
3 |
26033701 |
+ |
Nlgn1 |
NNNGTGGGCATGTCTAACTA |
NGG |
2 |
0.381 |
Tier II |
| 5 |
NC_000078.6 |
12 |
89007979 |
+ |
Nrxn3 |
NNNGTGGGACTGTCTAACAA |
NGG |
2 |
0.2381 |
Tier II |
| 6 |
NC_000071.6 |
5 |
142752273 |
+ |
Tnrc18 |
NNNGTGGGAATATCTAAGGA |
NGG |
2 |
0.1244 |
Tier II |
| 7 |
NC_000081.6 |
15 |
25784576 |
+ |
Myo10 |
NNNGTTGGAATGTCTAAGGA |
NGG |
2 |
0.0889 |
Tier II |
| 8 |
NC_000076.6 |
10 |
76150761 |
- |
Slc5a4a |
NNNGTGGGAATGTCTAACCA |
NGA |
2 |
0.0311 |
Tier II |
Other clones with same target sequence:
(none)
