Construct: sgRNA BRDN0001148223

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTCAGTGAGAAGGGGCACAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SBK1 (388228)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75800

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000016.10 16 28317477 - SBK1 NNNAGTGAGAAGGGGCACAC NGG 0 1.0 Tier I
2 NC_000013.11 13 42057592 - DGKH NNNACTGAGAAGGAGCACAC NGG 2 0.5893 Tier II
3 NC_000001.11 1 37626704 + RSPO1 NNNAGTGAGAAGAGGCACAA NGG 2 0.4615 Tier II
4 NC_000007.14 7 101958218 + CUX1 NNNGGTGGGAAGGGGCACAC NGG 2 0.4583 Tier II
5 NC_000015.10 15 90229424 - SEMA4B NNNGGTGGGAAGGGGCACAC NGG 2 0.4583 Tier II
6 NC_000010.11 10 62664281 + LOC124902436 NNNAGAGAGAAGGGGCACAA NGG 2 0.4333 Tier II
7 NC_000005.10 5 142927297 - ARHGAP26 NNNACTGAGAAGGGGCACAA NGG 2 0.3929 Tier II
8 NC_000015.10 15 68284660 - FEM1B NNNACTGAGAAGGGGCACAA NGG 2 0.3929 Tier II
9 NC_000007.14 7 138764259 + ATP6V0A4 NNNGGTGAGGAGGGGCACAC NGG 2 0.3472 Tier II
10 NC_000005.10 5 149840874 + PPARGC1B NNNAGGGAGGAGGGGCACAC NGG 2 0.3175 Tier II
11 NC_000007.14 7 134552160 - AKR1B15 NNNAGGGAGAAGGGGCACTC NGG 2 0.3077 Tier II
12 NC_000007.14 7 24764500 + GSDME NNNAGTGGGAATGGGCACAC NGG 2 0.2821 Tier II
13 NC_000007.14 7 24764500 + LOC124901820 NNNAGTGGGAATGGGCACAC NGG 2 0.2821 Tier II
14 NC_000007.14 7 116500461 + CAV2 NNNGGTGAGACGGGGCACAC NGG 2 0.25 Tier II
15 NC_000005.10 5 33441890 + TARS1 NNNAGTGAAAAGGGGCACGC NGG 2 0.2411 Tier II
16 NC_000001.11 1 169158056 - NME7 NNNAGTGAGTAGGGGCACAC NAG 2 0.2288 Tier II
17 NC_000022.11 22 46783429 + TBC1D22A NNNATTCAGAAGGGGCACAC NGG 2 0.2063 Tier II
18 NC_000011.10 11 114091939 - ZBTB16 NNNAGTGAGAAGGGGCGCAC NGG 1 0.1765 Tier II
19 NC_000018.10 18 11755700 + GNAL NNNAGTGAGGAGTGGCACAC NGG 2 0.1667 Tier II
20 NC_000006.12 6 161748717 + PRKN NNNAGTGAGAAGGGGCACTT NGG 2 0.1615 Tier II
21 NC_000007.14 7 132644476 - PLXNA4 NNNAGTGAGAAGGAGGACAC NGG 2 0.1154 Tier II
22 NC_000002.12 2 109319551 + RANBP2 NNNAGTGAGAGGGGGCTCAC NGG 2 0.0867 Tier II
23 NC_000002.12 2 109319551 + SH3RF3 NNNAGTGAGAGGGGGCTCAC NGG 2 0.0867 Tier II
24 NC_000014.9 14 104792089 + AKT1 NNNAGTGAGGAGGGGGACAC NGG 2 0.0855 Tier II
25 NC_000019.10 19 1293844 - EFNA2 NNNGGTGAGAAGGGGCAGAC NGG 2 0.0833 Tier II
26 NC_000012.12 12 76073771 + NAP1L1 NNNATTGAGAAGGGGCACAC NAG 2 0.0778 Tier II
27 NC_000016.10 16 25884994 + HS3ST4 NNNAGTGAGAAGGGGCAGTC NGG 2 0.0718 Tier II
28 NC_000003.12 3 30665809 - TGFBR2 NNNAGTGAGAAGGGGTCCAC NGG 2 0.0543 Tier II
29 NC_000006.12 6 43046632 + CUL7 NNNAGAGAGAAGGGGCACAG NGG 2 0.051 Tier II
30 NC_000001.11 1 21890414 - HSPG2 NNNAGTGAGAAGGGGCTCCC NGG 2 0.0276 Tier II
31 NC_000001.11 1 34169952 + C1orf94 NNNAGTGAGAAGGGGCCCAC NGC 2 0.0039 Tier II
32 NC_000004.12 4 160013469 - LOC107986324 NNNAGAGAGAAGGAGCACAC NGG 2 0.65 Tier III
33 NC_000007.14 7 134552160 - LOC105375520 NNNAGGGAGAAGGGGCACTC NGG 2 0.3077 Tier III
34 NC_000004.12 4 3783027 + LOC124900651 NNNAGCGAGAAGGGGCGCAC NGG 2 0.1604 Tier III
35 NC_000001.11 1 16165903 - EPHA2-AS1 NNNAGTGAGCAGGGGCCCAC NGG 2 0.0588 Tier III
36 NC_000019.10 19 10064708 + C3P1 NNNAGTGAGAAGGGGCTCAT NGG 2 0.04 Tier III
37 NC_000010.11 10 29100292 - LOC107984171 NNNAGTGAGAATGGGCACAC NTG 2 0.015 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 193330854 - Lamb3 NNNAGTGGGAAGGGGCACAC NGG 1 0.7333 Tier I
2 NC_000070.6 4 96010892 - Hook1 NNNAATGAGAAAGGGCACAC NGG 2 0.8089 Tier II
3 NC_000080.6 14 16365839 - Top2b NNNAGAGAGAAAGGGCACAC NGG 2 0.8089 Tier II
4 NC_000078.6 12 83471995 - Dpf3 NNNAGTGGGAAGGGGCACAC NGG 1 0.7333 Tier II
5 NC_000085.6 19 31709347 + Prkg1 NNNACAGAGAAGGGGCACAC NGG 2 0.681 Tier II
6 NC_000075.6 9 57959596 + Sema7a NNNAGTGAATAGGGGCACAC NGG 2 0.5672 Tier II
7 NC_000077.6 11 116064963 + Unc13d NNNAGAGAGAGGGGGCACAC NGG 2 0.5633 Tier II
8 NC_000073.6 7 64549566 - Apba2 NNNAGTGAGGAGGGGCACAC NGG 1 0.5556 Tier II
9 NC_000070.6 4 84593133 - Bnc2 NNNAGTGAGAACGGACACAC NGG 2 0.4983 Tier II
10 NC_000075.6 9 21433460 - Dnm2 NNNAGTGAGAGGGAGCACAC NGG 2 0.4875 Tier II
11 NC_000067.6 1 178911694 + Kif26b NNNAGTGAGAAGAGGCACAA NGG 2 0.4615 Tier II
12 NC_000075.6 9 101125414 + Ppp2r3a NNNAGTCAGAGGGGGCACAC NGG 2 0.4469 Tier II
13 NC_000075.6 9 67235190 - Tln2 NNNAGTGGGAACGGGCACAC NGG 2 0.3882 Tier II
14 NC_000073.6 7 24709427 + Lypd10 NNNAGGGAGAAGGGGCATAC NGG 2 0.3673 Tier II
15 NC_000084.6 18 61385578 + Ppargc1b NNNACTGAGAAGGCGCACAC NGG 2 0.3367 Tier II
16 NC_000083.6 17 56455893 + Ptprs NNNAGGTAGAAGGGGCACAC NGG 2 0.3265 Tier II
17 NC_000068.7 2 119400913 + Ino80 NNNAGTGAGAAGCAGCACAC NGG 2 0.3158 Tier II
18 NC_000067.6 1 78404293 - Sgpp2 NNNAGGGACAAGGGGCACAC NGG 2 0.3077 Tier II
19 NC_000067.6 1 133238353 + Plekha6 NNNAGTAAGATGGGGCACAC NGG 2 0.3077 Tier II
20 NC_000073.6 7 137115742 - Mgmt NNNAGAGAGATGGGGCACAC NGG 2 0.2667 Tier II
21 NC_000077.6 11 78483421 - Sarm1 NNNGGTGAGAAGGGGCACGC NGG 2 0.2344 Tier II
22 NC_000081.6 15 59339949 + Washc5 NNNAGGGAGAAGTGGCACAC NGG 2 0.1714 Tier II
23 NC_000077.6 11 64060651 + Cox10 NNNAGTGAGAAGGGACAGAC NGG 2 0.1255 Tier II
24 NC_000070.6 4 134846393 - Maco1 NNNAGTGAGACGGTGCACAC NGG 2 0.1067 Tier II
25 NC_000078.6 12 12311349 + Cyria NNNAGTGAGTAGGGGCACAG NGG 2 0.0519 Tier II
26 NC_000080.6 14 52144199 + Rpgrip1 NNNAGTCAGAAGGGGCACAC NTG 2 0.0268 Tier II
27 NC_000072.6 6 113491064 + Creld1 NNNAGTGAGAAGGGGCTGAC NGG 2 0.0178 Tier II
28 NC_000080.6 14 60848533 + Mipep NNNAGGGAGAAGGGGCACAC NGC 2 0.0127 Tier II
29 NC_000071.6 5 23742486 + Pus7 NNNCGTGAGAAGGGGCACAC NGC 2 0.0078 Tier II
30 NC_000076.6 10 19608088 - Ifngr1 NNNAGTGAGAAGGGTCACAC NTG 2 0.0056 Tier II
31 NC_000070.6 4 125189398 + Gm32611 NNNAGGGAGAAGGGGAACAC NGG 2 0.5714 Tier III
32 NC_000080.6 14 118719205 - Gm27198 NNNAGTGAGAAGGCACACAC NGG 2 0.4034 Tier III
33 NC_000069.6 3 134015188 - Gm26691 NNNAGTGAGAAGGGGAAGAC NGG 2 0.1333 Tier III
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Other clones with same target sequence:

(none)