Construct: sgRNA BRDN0001148226
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CACACATGACCAATAACAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K7 (6885)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75694
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
90556540 |
- |
MAP3K7 |
NNNACATGACCAATAACAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
98092668 |
+ |
TRIM14 |
NNNTCATGAACAATAACAAG |
NGG |
2 |
0.5515 |
Tier II |
| 3 |
NC_000007.14 |
7 |
155521305 |
+ |
CNPY1 |
NNNACATGAACAAAAACAAG |
NGG |
2 |
0.5365 |
Tier II |
| 4 |
NC_000007.14 |
7 |
50035896 |
+ |
ZPBP |
NNNATATGACCAAAAACAAG |
NGG |
2 |
0.3939 |
Tier II |
| 5 |
NC_000018.10 |
18 |
79805068 |
- |
KCNG2 |
NNNACATGGCCAATAACAGG |
NGG |
2 |
0.25 |
Tier II |
| 6 |
NC_000010.11 |
10 |
24325621 |
+ |
KIAA1217 |
NNNACATGACTAATTACAAG |
NGG |
2 |
0.0615 |
Tier II |
| 7 |
NC_000008.11 |
8 |
73426556 |
+ |
STAU2 |
NNNACATGAGCAAGAACAAG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000008.11 |
8 |
93826773 |
- |
TMEM67 |
NNNACATGTCCAAGAACAAG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000002.12 |
2 |
147845830 |
- |
ACVR2A |
NNNACATTACCAATACCAAG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000004.12 |
4 |
84297890 |
+ |
LINC02994 |
NNNAAAAGACCAATAACAAG |
NGG |
2 |
0.5 |
Tier III |
| 11 |
NC_000005.10 |
5 |
174366069 |
+ |
LINC01411 |
NNNACCTGAACAATAACAAG |
NGG |
2 |
0.3939 |
Tier III |
| 12 |
NC_000005.10 |
5 |
130375632 |
- |
LOC105379171 |
NNNACATGACCAATAATAAT |
NGG |
2 |
0.3267 |
Tier III |
| 13 |
NC_000008.11 |
8 |
73426556 |
+ |
STAU2-AS1 |
NNNACATGAGCAAGAACAAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
31981543 |
+ |
Map3k7 |
NNNACATGACCAATAATAAA |
NGG |
2 |
0.4375 |
Tier I |
| 2 |
NC_000072.6 |
6 |
125218665 |
+ |
Vamp1 |
NNNACATGACCAGTAACAGG |
NGG |
2 |
0.2446 |
Tier I |
| 3 |
NC_000084.6 |
18 |
39281744 |
- |
Arhgap26 |
NNNACATAACCAATGACAAG |
NGG |
2 |
0.65 |
Tier II |
| 4 |
NC_000069.6 |
3 |
80782601 |
- |
Gria2 |
NNNACATGAACAAAAACAAG |
NGG |
2 |
0.5365 |
Tier II |
| 5 |
NC_000074.6 |
8 |
81789963 |
- |
Inpp4b |
NNNACATGACAAATAACATG |
NGG |
2 |
0.4038 |
Tier II |
| 6 |
NC_000068.7 |
2 |
13292568 |
- |
Cubn |
NNNACATGACCAAAAATAAG |
NGG |
2 |
0.2889 |
Tier II |
| 7 |
NC_000076.6 |
10 |
92938999 |
+ |
Cfap54 |
NNNACATGGCCAATAACAAC |
NGG |
2 |
0.2857 |
Tier II |
| 8 |
NC_000078.6 |
12 |
80484173 |
- |
Exd2 |
NNNAAATGACCAATAAAAAG |
NGG |
2 |
0.2667 |
Tier II |
| 9 |
NC_000086.7 |
X |
87543530 |
- |
Il1rapl1 |
NNNAAATGACCAATAAAAAG |
NGG |
2 |
0.2667 |
Tier II |
| 10 |
NC_000084.6 |
18 |
80194661 |
- |
Rbfa |
NNNACATGACCAAAAACAAC |
NGG |
2 |
0.2653 |
Tier II |
| 11 |
NC_000076.6 |
10 |
32628650 |
+ |
Nkain2 |
NNNACAAGACCAATAACAAG |
NGA |
2 |
0.0608 |
Tier II |
| 12 |
NC_000086.7 |
X |
167912911 |
+ |
Frmpd4 |
NNNACATGACCAAAAACAAG |
NTG |
2 |
0.0241 |
Tier II |
| 13 |
NC_000068.7 |
2 |
141355104 |
- |
Macrod2 |
NNNACATGAACAATACCAAG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000076.6 |
10 |
82431229 |
+ |
Gm8188 |
NNNACATGACCAATAATAAA |
NGG |
2 |
0.4375 |
Tier III |
| 15 |
NC_000080.6 |
14 |
44963249 |
+ |
Gm16976 |
NNNACATGACCAATAACAGA |
NGG |
2 |
0.3516 |
Tier III |
| 16 |
NC_000068.7 |
2 |
116914936 |
- |
Gm46758 |
NNNAAAGGACCAATAACAAG |
NGG |
2 |
0.3361 |
Tier III |
| 17 |
NC_000068.7 |
2 |
13292568 |
- |
Gm13270 |
NNNACATGACCAAAAATAAG |
NGG |
2 |
0.2889 |
Tier III |
| 18 |
NC_000073.6 |
7 |
94743470 |
+ |
Gm32647 |
NNNACATGACCAATAACAAA |
NAG |
2 |
0.2431 |
Tier III |
| 19 |
NC_000073.6 |
7 |
84740652 |
- |
2610206C17Rik |
NNNACATGACTAAAAACAAG |
NGG |
2 |
0.1905 |
Tier III |
| 20 |
NC_000068.7 |
2 |
25554265 |
+ |
Gm35476 |
NNNACAGGACCAAGAACAAG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
