Construct: sgRNA BRDN0001148227

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCCGTAGTCTCCGATGCGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LMTK3 (114783)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77992

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 48501516 + LMTK3 NNNGTAGTCTCCGATGCGCA NGG 0 1.0 Tier I
2 NC_000013.11 13 27793990 - GSX1 NNNCTAGTCTCCGAGGCGCA NGG 2 0.0265 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000069.6 3 75147307 - Zbbx NNNGTAGTCTCCCATGCCCA NGG 2 0.2005 Tier II
2 NC_000082.6 16 18591793 + Gm35369 NNNGAAGTCTCCGAGGCGCA NGG 2 0.025 Tier III
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Other clones with same target sequence:

(none)