Construct: sgRNA BRDN0001148232

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAAGATAGTCAATTGCTCGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDKL3 (51265)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75606

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 134359925 + CDKL3 NNNGATAGTCAATTGCTCGA NGG 0 1.0 Tier I
2 NC_000016.10 16 25796142 - HS3ST4 NNNGATAGGCAATTGCACGA NGG 2 0.3302 Tier II
3 NC_000022.11 22 44103573 - PARVB NNNGATAGTCAATGGCTCCA NGG 2 0.0 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 161988363 - Dnm3 NNNGATACTCAATTCCTCGA NGG 2 0.1678 Tier II
2 NC_000069.6 3 145098737 - Clca2 NNNGATAGTCAATTGATCGA NTG 2 0.039 Tier II
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Other clones with same target sequence:

(none)