Construct: sgRNA BRDN0001148237
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATGGAAGAGCGTCATGACTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- Negative control (unspec'd)
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80253
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
26476911 |
+ |
OTOF |
NNNGAAGAGCGTCTTGACCT |
NGG |
2 |
0.1471 |
Tier I |
| 2 |
NC_000002.12 |
2 |
171922780 |
+ |
HAT1 |
NNNGAAAAGCGTCATGATTT |
NGG |
2 |
0.6429 |
Tier II |
| 3 |
NC_000007.14 |
7 |
21678155 |
- |
DNAH11 |
NNNGAAAAGCTTCATGACTT |
NGG |
2 |
0.3846 |
Tier II |
| 4 |
NC_000002.12 |
2 |
200640999 |
- |
AOX1 |
NNNGAAAAGCGTCATGACAT |
NGG |
2 |
0.2857 |
Tier II |
| 5 |
NC_000005.10 |
5 |
78375435 |
- |
SCAMP1 |
NNNGAAGAGCTTTATGACTT |
NGG |
2 |
0.2692 |
Tier II |
| 6 |
NC_000010.11 |
10 |
100972297 |
+ |
SEMA4G |
NNNGAAGAGGGTCATGACCT |
NGG |
2 |
0.1073 |
Tier II |
| 7 |
NC_000019.10 |
19 |
9993087 |
+ |
COL5A3 |
NNNGAAAAGCGTCATTACTT |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000013.11 |
13 |
51012073 |
- |
GUCY1B2 |
NNNGAATAGCATCATGACTT |
NGG |
2 |
0.5714 |
Tier III |
| 9 |
NC_000016.10 |
16 |
63460324 |
+ |
LOC105371308 |
NNNTAAGAGCATCATGACTT |
NGG |
2 |
0.3636 |
Tier III |
| 10 |
NC_000023.11 |
X |
113323124 |
+ |
LOC101928437 |
NNNGAAGAGCTTCATGACAT |
NGG |
2 |
0.1099 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
30383541 |
+ |
Otof |
NNNGAAGAGGGTCTTGACTT |
NGG |
2 |
0.2074 |
Tier I |
| 2 |
NC_000070.6 |
4 |
34955485 |
+ |
Mob3b |
NNNGAAGAGCATCATGACTT |
NGG |
1 |
1.0 |
Tier II |
| 3 |
NC_000076.6 |
10 |
21976253 |
- |
Sgk1 |
NNNGGAGATCGTCATGACTT |
NGG |
2 |
0.384 |
Tier II |
| 4 |
NC_000073.6 |
7 |
107437724 |
- |
Syt9 |
NNNGAAGAGCGTCATGATTA |
NGG |
2 |
0.3616 |
Tier II |
| 5 |
NC_000080.6 |
14 |
29198631 |
- |
Cacna2d3 |
NNNGAAGGGCGTCATGACTT |
NGA |
2 |
0.0509 |
Tier II |
| 6 |
NC_000084.6 |
18 |
50076255 |
- |
Tnfaip8 |
NNNGAAGAGCGTGATGACAT |
NGG |
2 |
0.039 |
Tier II |
| 7 |
NC_000083.6 |
17 |
10600871 |
- |
Pacrg |
NNNGCAGAGCGTCATGACTT |
NGC |
2 |
0.0111 |
Tier II |
Other clones with same target sequence:
(none)
