Construct: sgRNA BRDN0001148238
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTAATGGTATCTCTATAACT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MPP6 (51678)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76773
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
24650686 |
- |
PALS2 |
NNNATGGTATCTCTATAACT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
110604577 |
- |
ARHGAP20 |
NNNAGGGTATCTTTATAACT |
NGG |
2 |
0.448 |
Tier II |
| 3 |
NC_000020.11 |
20 |
8769830 |
- |
PLCB1 |
NNNATCTTATCTCTATAACT |
NGG |
2 |
0.3896 |
Tier II |
| 4 |
NC_000002.12 |
2 |
169484703 |
- |
BBS5 |
NNNATTTTATCTCTATAACT |
NGG |
2 |
0.381 |
Tier II |
| 5 |
NC_000001.11 |
1 |
185162424 |
- |
SWT1 |
NNNATGGTATCTCTAAAGCT |
NGG |
2 |
0.3636 |
Tier II |
| 6 |
NC_000015.10 |
15 |
56129728 |
+ |
RFX7 |
NNNATGGTATTTCTAAAACT |
NGG |
2 |
0.2797 |
Tier II |
| 7 |
NC_000008.11 |
8 |
112567619 |
+ |
CSMD3 |
NNNATGATATCTCTATAACT |
NAG |
2 |
0.2593 |
Tier II |
| 8 |
NC_000001.11 |
1 |
108921028 |
- |
GPSM2 |
NNNATGGTATCCCTTTAACT |
NGG |
2 |
0.1895 |
Tier II |
| 9 |
NC_000007.14 |
7 |
96672504 |
+ |
SEM1 |
NNNATGGTGTCTCTATGACT |
NGG |
2 |
0.1176 |
Tier II |
| 10 |
NC_000005.10 |
5 |
26894324 |
+ |
CDH9 |
NNNATGGTATTTCTATAACT |
NAG |
2 |
0.0798 |
Tier II |
| 11 |
NC_000016.10 |
16 |
14038012 |
+ |
MRTFB |
NNNATGGTATCTCCATACCT |
NGG |
2 |
0.0544 |
Tier II |
| 12 |
NC_000005.10 |
5 |
112411072 |
+ |
EPB41L4A |
NNNATGGTATCTCCATAACT |
NGT |
2 |
0.0046 |
Tier II |
| 13 |
NC_000003.12 |
3 |
160232431 |
+ |
TRIM59-IFT80 |
NNNATGGTATCTCTTTTACT |
NGG |
2 |
0.0267 |
Tier III |
| 14 |
NC_000003.12 |
3 |
19720926 |
+ |
LOC105376984 |
NNNTTGGTATCTCTATAACT |
NTG |
2 |
0.0248 |
Tier III |
| 15 |
NC_000006.12 |
6 |
123648659 |
+ |
LOC105377981 |
NNNATTGTATCTCTATAACT |
NGC |
2 |
0.0148 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
50163623 |
- |
Mpp6 |
NNNATAGTATCTCTGTAACT |
NGG |
2 |
0.65 |
Tier I |
| 2 |
NC_000071.6 |
5 |
49976180 |
+ |
Adgra3 |
NNNATGGTAACTTTATAACT |
NGG |
2 |
0.6 |
Tier II |
| 3 |
NC_000084.6 |
18 |
12202008 |
- |
Npc1 |
NNNATGTTAACTCTATAACT |
NGG |
2 |
0.4898 |
Tier II |
| 4 |
NC_000076.6 |
10 |
123541744 |
+ |
Tafa2 |
NNNATGCTGTCTCTATAACT |
NGG |
2 |
0.4583 |
Tier II |
| 5 |
NC_000072.6 |
6 |
62248235 |
+ |
Ccser1 |
NNNATGGTGTCTCTATAACA |
NGG |
2 |
0.375 |
Tier II |
| 6 |
NC_000079.6 |
13 |
103142698 |
+ |
Mast4 |
NNNATGGTTTCTCTATAACA |
NGG |
2 |
0.3375 |
Tier II |
| 7 |
NC_000079.6 |
13 |
18370904 |
+ |
Pou6f2 |
NNNATGGTATCTACATAACT |
NGG |
2 |
0.1099 |
Tier II |
| 8 |
NC_000067.6 |
1 |
126662838 |
+ |
Nckap5 |
NNNATGTTATCTGTATAACT |
NGG |
2 |
0.0779 |
Tier II |
| 9 |
NC_000070.6 |
4 |
66394015 |
- |
Astn2 |
NNNATGTTATCTCTATAAGT |
NGG |
2 |
0.0714 |
Tier II |
| 10 |
NC_000084.6 |
18 |
45718501 |
- |
A330093E20Rik |
NNNATCGTATCTCTCTAACT |
NGG |
2 |
0.186 |
Tier III |
| 11 |
NC_000077.6 |
11 |
67840905 |
+ |
Gm40032 |
NNNATGGTATCTTTATGACT |
NGG |
2 |
0.1235 |
Tier III |
| 12 |
NC_000085.6 |
19 |
56167010 |
+ |
Gm31965 |
NNNATGGTATCTCAATCACT |
NGG |
2 |
0.1092 |
Tier III |
Other clones with same target sequence:
(none)
