Construct: sgRNA BRDN0001148239

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAGTCGCGGAGCCGCTCGCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HUNK (30811)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75940

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000021.9 21 31873813 - HUNK NNNTCGCGGAGCCGCTCGCG NGG 0 1.0 Tier I
2 NC_000020.11 20 63642126 + STMN3 NNNTCGCGCAGCCGCTCGCG NAG 2 0.1396 Tier I
3 NC_000010.11 10 102231012 - PITX3 NNNTCGCGGCGCCGCTCGGG NGG 2 0.0417 Tier I
4 NC_000022.11 22 25179384 + KIAA1671 NNNTCGCGCAGCCGCTCGCG NAG 2 0.1396 Tier II
5 NC_000005.10 5 179907835 + TBC1D9B NNNTCGCGGAGCCGCTCGCA NCG 2 0.1004 Tier II
6 NC_000003.12 3 197029105 - MELTF NNNTCGGGGAGCGGCTCGCG NGG 2 0.0642 Tier II
7 NC_000010.11 10 102231012 - GBF1 NNNTCGCGGCGCCGCTCGGG NGG 2 0.0417 Tier II
8 NC_000022.11 22 25179384 + STMN3P1 NNNTCGCGCAGCCGCTCGCG NAG 2 0.1396 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 181307234 + Stmn3 NNNTCGCGCAGCCGCTCGCG NAG 2 0.1396 Tier I
2 NC_000082.6 16 90386594 - Hunk NNNTCCCGGAGCCGCTCGCG NGA 2 0.0473 Tier I
3 NC_000081.6 15 10649783 - Rai14 NNNTCCAGGAGCCGCTCGCG NGG 2 0.5114 Tier II
4 NC_000068.7 2 168741891 + Atp9a NNNGCGCGGAGCCGCCCGCG NGG 2 0.4167 Tier II
5 NC_000076.6 10 70097093 + Ccdc6 NNNTGGCGGAGCAGCTCGCG NGG 2 0.2308 Tier II
6 NC_000076.6 10 70097093 + Gm51771 NNNTGGCGGAGCAGCTCGCG NGG 2 0.2308 Tier III
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Other clones with same target sequence:

(none)