Construct: sgRNA BRDN0001148239
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGTCGCGGAGCCGCTCGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- HUNK (30811)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75940
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000021.9 |
21 |
31873813 |
- |
HUNK |
NNNTCGCGGAGCCGCTCGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
63642126 |
+ |
STMN3 |
NNNTCGCGCAGCCGCTCGCG |
NAG |
2 |
0.1396 |
Tier I |
| 3 |
NC_000010.11 |
10 |
102231012 |
- |
PITX3 |
NNNTCGCGGCGCCGCTCGGG |
NGG |
2 |
0.0417 |
Tier I |
| 4 |
NC_000022.11 |
22 |
25179384 |
+ |
KIAA1671 |
NNNTCGCGCAGCCGCTCGCG |
NAG |
2 |
0.1396 |
Tier II |
| 5 |
NC_000005.10 |
5 |
179907835 |
+ |
TBC1D9B |
NNNTCGCGGAGCCGCTCGCA |
NCG |
2 |
0.1004 |
Tier II |
| 6 |
NC_000003.12 |
3 |
197029105 |
- |
MELTF |
NNNTCGGGGAGCGGCTCGCG |
NGG |
2 |
0.0642 |
Tier II |
| 7 |
NC_000010.11 |
10 |
102231012 |
- |
GBF1 |
NNNTCGCGGCGCCGCTCGGG |
NGG |
2 |
0.0417 |
Tier II |
| 8 |
NC_000022.11 |
22 |
25179384 |
+ |
LOC101232810 |
NNNTCGCGCAGCCGCTCGCG |
NAG |
2 |
0.1396 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
181307234 |
+ |
Stmn3 |
NNNTCGCGCAGCCGCTCGCG |
NAG |
2 |
0.1396 |
Tier I |
| 2 |
NC_000082.6 |
16 |
90386594 |
- |
Hunk |
NNNTCCCGGAGCCGCTCGCG |
NGA |
2 |
0.0473 |
Tier I |
| 3 |
NC_000081.6 |
15 |
10649783 |
- |
Rai14 |
NNNTCCAGGAGCCGCTCGCG |
NGG |
2 |
0.5114 |
Tier II |
| 4 |
NC_000068.7 |
2 |
168741891 |
+ |
Atp9a |
NNNGCGCGGAGCCGCCCGCG |
NGG |
2 |
0.4167 |
Tier II |
| 5 |
NC_000076.6 |
10 |
70097093 |
+ |
Ccdc6 |
NNNTGGCGGAGCAGCTCGCG |
NGG |
2 |
0.2308 |
Tier II |
| 6 |
NC_000076.6 |
10 |
70097093 |
+ |
Gm51771 |
NNNTGGCGGAGCAGCTCGCG |
NGG |
2 |
0.2308 |
Tier III |
Other clones with same target sequence:
(none)
