Construct: sgRNA BRDN0001148240
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACCTTCTGATCAGTGACACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK14 (5218)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76640
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
90917697 |
+ |
CDK14 |
NNNTTCTGATCAGTGACACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
55396838 |
+ |
PCDH15 |
NNNTTCTGAACAGTGACACA |
NGG |
2 |
0.8036 |
Tier II |
| 3 |
NC_000004.12 |
4 |
90886683 |
- |
CCSER1 |
NNNTTCTGATCAGTAAAACG |
NGG |
2 |
0.4392 |
Tier II |
| 4 |
NC_000004.12 |
4 |
183874251 |
+ |
STOX2 |
NNNTTCTTATCAGTGACACT |
NGG |
2 |
0.4375 |
Tier II |
| 5 |
NC_000005.10 |
5 |
11703846 |
- |
CTNND2 |
NNNTTCTGATCAGAGATACG |
NGG |
2 |
0.2889 |
Tier II |
| 6 |
NC_000012.12 |
12 |
125139242 |
+ |
AACS |
NNNCTCTGATCTGTGACACG |
NGG |
2 |
0.2157 |
Tier II |
| 7 |
NC_000006.12 |
6 |
158624642 |
- |
TMEM181 |
NNNTTCTTATCTGTGACACG |
NGG |
2 |
0.2083 |
Tier II |
| 8 |
NC_000002.12 |
2 |
3262823 |
- |
EIPR1 |
NNNTTCTCATGAGTGACACG |
NGG |
2 |
0.1538 |
Tier II |
| 9 |
NC_000011.10 |
11 |
92697715 |
- |
FAT3 |
NNNTTCTGATTATTGACACG |
NGG |
2 |
0.0923 |
Tier II |
| 10 |
NC_000001.11 |
1 |
3882998 |
+ |
DFFB |
NNNTTCTGAACAGTGACACG |
NGT |
2 |
0.0138 |
Tier II |
| 11 |
NC_000004.12 |
4 |
7253404 |
+ |
SORCS2 |
NNNTTCTGAGCAGTGACACG |
NGC |
2 |
0.0111 |
Tier II |
| 12 |
NC_000003.12 |
3 |
82224713 |
+ |
LINC02008 |
NNNTTCTGATCAGTGGCACT |
NGG |
2 |
0.1346 |
Tier III |
| 13 |
NC_000010.11 |
10 |
4234888 |
- |
LINC00702 |
NNNTTCTGATCATTGCCACG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
11756186 |
+ |
A830018L16Rik |
NNNTTCTGCTCAGTGACACA |
NGG |
2 |
0.5357 |
Tier II |
| 2 |
NC_000069.6 |
3 |
151509457 |
- |
Adgrl4 |
NNNTTCTTTTCAGTGACACG |
NGG |
2 |
0.375 |
Tier II |
| 3 |
NC_000068.7 |
2 |
15062670 |
+ |
Arl5b |
NNNTTCTGTTCAGTGACATG |
NGG |
2 |
0.2769 |
Tier II |
| 4 |
NC_000068.7 |
2 |
28989856 |
- |
Cfap77 |
NNNTTCTGAAGAGTGACACG |
NGG |
2 |
0.2143 |
Tier II |
| 5 |
NC_000082.6 |
16 |
66849036 |
- |
Cadm2 |
NNNTTCTGATCAGTTACACC |
NGG |
2 |
0.0612 |
Tier II |
| 6 |
NC_000083.6 |
17 |
81511577 |
- |
Slc8a1 |
NNNTTCTGCTCAGTGACACG |
NGA |
2 |
0.0397 |
Tier II |
| 7 |
NC_000075.6 |
9 |
70892987 |
- |
Lipc |
NNNTTCTGATCAGTGAGAAG |
NGG |
2 |
0.0252 |
Tier II |
| 8 |
NC_000083.6 |
17 |
30977820 |
- |
Umodl1 |
NNNTTCTGATCAGGGACACG |
NGG |
1 |
0.0 |
Tier II |
| 9 |
NC_000072.6 |
6 |
91411977 |
+ |
Gm30437 |
NNNTTCTGATCAGAGACACG |
NAG |
2 |
0.1605 |
Tier III |
| 10 |
NC_000074.6 |
8 |
36210267 |
+ |
Gm39165 |
NNNTTCTGATCAGTGTCACT |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
