Construct: sgRNA BRDN0001148259
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTTACCCGGAGGAACTTCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NME2 (4831)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77934
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
51166946 |
- |
NME2 |
NNNTACCCGGAGGAACTTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
51166946 |
- |
NME1-NME2 |
NNNTACCCGGAGGAACTTCA |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000019.10 |
19 |
15362121 |
+ |
AKAP8 |
NNNTACCTGGAGGAACTCCA |
NGG |
2 |
0.375 |
Tier I |
| 4 |
NC_000007.14 |
7 |
711364 |
+ |
PRKAR1B |
NNNCTCCCGGAGGAACTTCA |
NGG |
2 |
0.2353 |
Tier I |
| 5 |
NC_000001.11 |
1 |
9054145 |
+ |
SLC2A5 |
NNNGACACGGAGGAACTTCA |
NGG |
2 |
0.4688 |
Tier II |
| 6 |
NC_000011.10 |
11 |
44851574 |
- |
TSPAN18 |
NNNGACCCAGAGGAACTTCA |
NGG |
2 |
0.4018 |
Tier II |
| 7 |
NC_000016.10 |
16 |
78240620 |
+ |
WWOX |
NNNTTCCCGGAGGAACTTCA |
NAG |
2 |
0.0943 |
Tier II |
| 8 |
NC_000009.12 |
9 |
4036798 |
+ |
GLIS3 |
NNNTACCAGGAGGAACTTCA |
NGA |
2 |
0.0451 |
Tier II |
| 9 |
NC_000019.10 |
19 |
15362121 |
+ |
LOC124904643 |
NNNTACCTGGAGGAACTCCA |
NGG |
2 |
0.375 |
Tier III |
| 10 |
NC_000002.12 |
2 |
191532566 |
+ |
LOC107985833 |
NNNTACCCTGAGGAACTTTA |
NGG |
2 |
0.2462 |
Tier III |
| 11 |
NC_000016.10 |
16 |
78240620 |
+ |
WWOX-AS1 |
NNNTTCCCGGAGGAACTTCA |
NAG |
2 |
0.0943 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
93955506 |
+ |
Nme2 |
NNNTACCCGAAGGAACTTCA |
NGG |
1 |
0.9333 |
Tier I |
| 2 |
NC_000080.6 |
14 |
25500168 |
+ |
Zmiz1 |
NNNTACCCGGAGGTACTTCA |
NAG |
2 |
0.1383 |
Tier II |
| 3 |
NC_000081.6 |
15 |
93016013 |
+ |
Gm30085 |
NNNTACCTGGAGGAACTTCA |
NTG |
2 |
0.0341 |
Tier III |
Other clones with same target sequence:
(none)
