Construct: sgRNA BRDN0001148262
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGGAACCTGCGGTCCTCAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SCYL2 (55681)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76051
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
100326741 |
+ |
SCYL2 |
NNNGAACCTGCGGTCCTCAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
143934848 |
+ |
PLEC |
NNNGAACCTGCGTTCCTCAA |
NGG |
2 |
0.1687 |
Tier I |
| 3 |
NC_000010.11 |
10 |
67054395 |
- |
CTNNA3 |
NNNGATCCTGCTGTCCTCAT |
NGG |
2 |
0.2747 |
Tier II |
| 4 |
NC_000010.11 |
10 |
67054395 |
- |
LRRTM3 |
NNNGATCCTGCTGTCCTCAT |
NGG |
2 |
0.2747 |
Tier II |
| 5 |
NC_000001.11 |
1 |
9362273 |
- |
SPSB1 |
NNNGAGCCTGCGGTCCTCAT |
NTG |
2 |
0.0278 |
Tier II |
| 6 |
NC_000010.11 |
10 |
24631601 |
- |
ARHGAP21 |
NNNGAACCTGTGGTTCTCAT |
NGG |
2 |
0.0205 |
Tier II |
| 7 |
NC_000005.10 |
5 |
12467479 |
+ |
LOC105374655 |
NNNGAACCTGCTCTCCTCAT |
NGG |
2 |
0.1619 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
89650842 |
- |
Scyl2 |
NNNGAGCCTGCAGTCCTCAT |
NGG |
2 |
0.6667 |
Tier I |
| 2 |
NC_000067.6 |
1 |
65926661 |
- |
Crygf |
NNNGAAGCTGCGGTCCTCAT |
NGA |
2 |
0.0327 |
Tier I |
| 3 |
NC_000073.6 |
7 |
27213998 |
+ |
Itpkc |
NNNGAATCTGCAGTCCTCAT |
NGG |
2 |
0.7583 |
Tier II |
| 4 |
NC_000067.6 |
1 |
93229099 |
- |
Crocc2 |
NNNGAAGCTGAGGTCCTCAT |
NGG |
2 |
0.3529 |
Tier II |
| 5 |
NC_000072.6 |
6 |
90359041 |
+ |
Uroc1 |
NNNGAACATGCGGTCCTCTT |
NGG |
2 |
0.35 |
Tier II |
| 6 |
NC_000081.6 |
15 |
80186544 |
+ |
Mgat3 |
NNNGAACCTGCAGTCCTCAT |
NGC |
2 |
0.0207 |
Tier II |
| 7 |
NC_000070.6 |
4 |
126236156 |
- |
Map7d1 |
NNNGAACCTGTGGTCCTCAT |
NGT |
2 |
0.005 |
Tier II |
| 8 |
NC_000077.6 |
11 |
83541471 |
+ |
Gm32650 |
NNNGCACCTGCTGTCCTCAT |
NGG |
2 |
0.1923 |
Tier III |
| 9 |
NC_000067.6 |
1 |
86299622 |
- |
Gm16341 |
NNNGAACCTCTGGTCCTCAT |
NGG |
2 |
0.1231 |
Tier III |
Other clones with same target sequence:
(none)
