Construct: sgRNA BRDN0001148276
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATCAATCAAGGAATTGACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LRRK1 (79705)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75716
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
101049755 |
- |
LRRK1 |
NNNCAATCAAGGAATTGACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
35200144 |
- |
PRORP |
NNNCAATCATGGAATTGTCG |
NGG |
2 |
0.4412 |
Tier II |
| 3 |
NC_000002.12 |
2 |
7029242 |
- |
RNF144A |
NNNCAATGAAGGAAATGACG |
NGG |
2 |
0.3722 |
Tier II |
| 4 |
NC_000007.14 |
7 |
143900557 |
- |
TCAF1 |
NNNCAATCAAGGTATTGACT |
NGG |
2 |
0.21 |
Tier II |
| 5 |
NC_000008.11 |
8 |
96548057 |
- |
SDC2 |
NNNCAATCAAGGACTTGGCG |
NGG |
2 |
0.0857 |
Tier II |
| 6 |
NC_000006.12 |
6 |
127127655 |
+ |
RSPO3 |
NNNCACTCAAGGAATTGAGG |
NGG |
2 |
0.0568 |
Tier II |
| 7 |
NC_000015.10 |
15 |
101049755 |
- |
LOC105371026 |
NNNCAATCAAGGAATTGACG |
NGG |
0 |
1.0 |
Tier III |
| 8 |
NC_000023.11 |
X |
23283111 |
+ |
PTCHD1-AS |
NNNCAAACAAGGAATTAACG |
NGG |
2 |
0.8167 |
Tier III |
| 9 |
NC_000014.9 |
14 |
35200144 |
- |
PRORP-PSMA6 |
NNNCAATCATGGAATTGTCG |
NGG |
2 |
0.4412 |
Tier III |
| 10 |
NC_000007.14 |
7 |
143900557 |
- |
LOC105375549 |
NNNCAATCAAGGTATTGACT |
NGG |
2 |
0.21 |
Tier III |
| 11 |
NC_000008.11 |
8 |
96548057 |
- |
LOC124900253 |
NNNCAATCAAGGACTTGGCG |
NGG |
2 |
0.0857 |
Tier III |
| 12 |
NC_000006.12 |
6 |
127127655 |
+ |
LOC105377989 |
NNNCACTCAAGGAATTGAGG |
NGG |
2 |
0.0568 |
Tier III |
| 13 |
NC_000008.11 |
8 |
81881128 |
+ |
LINC02235 |
NNNCACTCAAGGAATTGACG |
NGT |
2 |
0.0073 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
88964366 |
- |
Nrxn3 |
NNNCAATCAAGGATTTGACA |
NGG |
2 |
0.5 |
Tier II |
| 2 |
NC_000078.6 |
12 |
88964366 |
- |
Gm40537 |
NNNCAATCAAGGATTTGACA |
NGG |
2 |
0.5 |
Tier III |
Other clones with same target sequence:
(none)
