Construct: sgRNA BRDN0001148279
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGCGAGACTGCTTGTCTCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIP5KL1 (138429)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75824
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
127929812 |
+ |
PIP5KL1 |
NNNGAGACTGCTTGTCTCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
39390005 |
- |
PAF1 |
NNNGGGACTGCTTGTCTCGT |
NGG |
2 |
0.504 |
Tier II |
| 3 |
NC_000019.10 |
19 |
39390005 |
- |
SAMD4B |
NNNGGGACTGCTTGTCTCGT |
NGG |
2 |
0.504 |
Tier II |
| 4 |
NC_000015.10 |
15 |
85794386 |
- |
KLHL25 |
NNNGAGACTGCTTGTCTTTG |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000010.11 |
10 |
84252237 |
- |
RGR |
NNNGAGAATGCTTGACTCGG |
NGG |
2 |
0.3763 |
Tier II |
| 6 |
NC_000017.11 |
17 |
19322982 |
+ |
EPN2 |
NNNGAGACTAGTTGTCTCGG |
NGG |
2 |
0.2333 |
Tier II |
| 7 |
NC_000019.10 |
19 |
54906909 |
+ |
NCR1 |
NNNGACACTGCTTGTCTCGG |
NAG |
2 |
0.1768 |
Tier II |
| 8 |
NC_000018.10 |
18 |
75243511 |
+ |
TSHZ1 |
NNNGATACTGCTTGTCTGGG |
NGG |
2 |
0.0889 |
Tier II |
| 9 |
NC_000019.10 |
19 |
5901156 |
- |
NDUFA11 |
NNNGAGACAGCTTGTCTCGG |
NGT |
2 |
0.015 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
21186464 |
+ |
Asap2 |
NNNGAAACTGCTTGTCTTGG |
NGG |
2 |
0.6429 |
Tier II |
| 2 |
NC_000069.6 |
3 |
92365625 |
+ |
Sprr2f |
NNNGAGACTGCCTGTCTTGG |
NGG |
2 |
0.609 |
Tier II |
| 3 |
NC_000076.6 |
10 |
86164449 |
- |
Syn3 |
NNNGAGGCTGCTTGTCTAGG |
NGG |
2 |
0.3801 |
Tier II |
| 4 |
NC_000084.6 |
18 |
24789339 |
+ |
Fhod3 |
NNNGAGACACCTTGTCTCGG |
NGG |
2 |
0.3714 |
Tier II |
| 5 |
NC_000068.7 |
2 |
60379536 |
- |
Ly75 |
NNNGAGAATGCTTGTCTCGC |
NGG |
2 |
0.2786 |
Tier II |
| 6 |
NC_000067.6 |
1 |
182626306 |
+ |
Capn8 |
NNNGAGACTGGTTGTCTGGG |
NGG |
2 |
0.0333 |
Tier II |
| 7 |
NC_000071.6 |
5 |
44743847 |
+ |
Ldb2 |
NNNGAGAATGCTTGTCTCGG |
NTG |
2 |
0.0253 |
Tier II |
| 8 |
NC_000070.6 |
4 |
62360102 |
- |
Fkbp15 |
NNNGAGACTGCTTGCCTCGG |
NGT |
2 |
0.0044 |
Tier II |
| 9 |
NC_000076.6 |
10 |
82192941 |
- |
Gm40720 |
NNNGAGACAGCTTGACTCGG |
NGG |
2 |
0.5376 |
Tier III |
| 10 |
NC_000081.6 |
15 |
16616928 |
+ |
Gm2611 |
NNNGAGACAGCTTGTCTAGG |
NGG |
2 |
0.5 |
Tier III |
| 11 |
NC_000076.6 |
10 |
86164449 |
- |
Gm35636 |
NNNGAGGCTGCTTGTCTAGG |
NGG |
2 |
0.3801 |
Tier III |
| 12 |
NC_000075.6 |
9 |
41569211 |
+ |
Mir100hg |
NNNGAGACTGCTTGCCTGGG |
NGG |
2 |
0.0364 |
Tier III |
| 13 |
NC_000079.6 |
13 |
71566401 |
+ |
1700112M02Rik |
NNNGAGACTGCTTGTCTCAG |
NGT |
2 |
0.0115 |
Tier III |
Other clones with same target sequence:
(none)
