Construct: sgRNA BRDN0001148281
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTGCGGTCTTTCGACCACTC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PANK4 (55229)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76970
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
2521728 |
- |
PANK4 |
NNNCGGTCTTTCGACCACTC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
34147970 |
- |
CSMD2 |
NNNCTGGCTTTCGACCACTC |
NGG |
2 |
0.1765 |
Tier II |
| 3 |
NC_000010.11 |
10 |
11334661 |
+ |
CELF2 |
NNNCGGTCTTTTGACCACAC |
NGG |
2 |
0.1538 |
Tier II |
| 4 |
NC_000006.12 |
6 |
116060800 |
+ |
FRK |
NNNCTGTCTTTCGACCAGTC |
NGG |
2 |
0.04 |
Tier II |
| 5 |
NC_000019.10 |
19 |
13815515 |
+ |
ZSWIM4 |
NNNCGGTCTTTCGCCCAGTC |
NGG |
2 |
0.0286 |
Tier II |
| 6 |
NC_000022.11 |
22 |
37930041 |
- |
MICALL1 |
NNNGGGTCTTTCGAGCACTC |
NGG |
2 |
0.025 |
Tier II |
| 7 |
NC_000010.11 |
10 |
11334661 |
+ |
CELF2-AS1 |
NNNCGGTCTTTTGACCACAC |
NGG |
2 |
0.1538 |
Tier III |
| 8 |
NC_000022.11 |
22 |
22124161 |
- |
IGL |
NNNCTGTCTTTCTACCACTC |
NGG |
2 |
0.09 |
Tier III |
| 9 |
NC_000019.10 |
19 |
13815515 |
+ |
LOC107985334 |
NNNCGGTCTTTCGCCCAGTC |
NGG |
2 |
0.0286 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
4916896 |
+ |
Mgrn1 |
NNNCGGTCTCTAGACCACTC |
NGG |
2 |
0.381 |
Tier II |
Other clones with same target sequence:
(none)
