Construct: sgRNA BRDN0001148283
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTTTGCCACCTATTCCGAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ULK4 (54986)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76008
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
41918462 |
- |
ULK4 |
NNNTTGCCACCTATTCCGAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
68224448 |
+ |
TAFA1 |
NNNTTGCAACCTATTCCGAA |
NGG |
1 |
0.65 |
Tier II |
| 3 |
NC_000005.10 |
5 |
137124439 |
+ |
SPOCK1 |
NNNTTGCCTCCTATTCTGAA |
NGG |
2 |
0.28 |
Tier II |
| 4 |
NC_000009.12 |
9 |
105131879 |
- |
LOC105376197 |
NNNTTGTCACCTATTCTGAA |
NGG |
2 |
0.3792 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
127229479 |
- |
Snrnp200 |
NNNCTGCCACCTATTCAGAA |
NGG |
2 |
0.302 |
Tier I |
| 2 |
NC_000070.6 |
4 |
144405901 |
+ |
Pramel13 |
NNNTTGCTACCTATTCCAAA |
NGG |
2 |
0.6058 |
Tier II |
| 3 |
NC_000073.6 |
7 |
78458430 |
+ |
Ntrk3 |
NNNTTGCCACCAATTCCTAA |
NGG |
2 |
0.5333 |
Tier II |
| 4 |
NC_000069.6 |
3 |
87628435 |
- |
Arhgef11 |
NNNTTGCCACATATTCCAAA |
NGG |
2 |
0.5192 |
Tier II |
| 5 |
NC_000074.6 |
8 |
108286311 |
+ |
Zfhx3 |
NNNTTGCCACCTGTTCCGAG |
NGG |
2 |
0.4987 |
Tier II |
| 6 |
NC_000071.6 |
5 |
132222695 |
- |
Auts2 |
NNNTTGCCACCTGTTCCTAA |
NGG |
2 |
0.4348 |
Tier II |
| 7 |
NC_000087.7 |
Y |
1114745 |
- |
Uty |
NNNTTGCCACCTATTCCAGA |
NGG |
2 |
0.2596 |
Tier II |
| 8 |
NC_000074.6 |
8 |
125200992 |
- |
Disc1 |
NNNTTGCCACCTATTTAGAA |
NGG |
2 |
0.1436 |
Tier II |
| 9 |
NC_000084.6 |
18 |
17057579 |
+ |
1700001G01Rik |
NNNTTGCTACCTATTCCAAA |
NGG |
2 |
0.6058 |
Tier III |
| 10 |
NC_000084.6 |
18 |
17057579 |
+ |
Gm41674 |
NNNTTGCTACCTATTCCAAA |
NGG |
2 |
0.6058 |
Tier III |
Other clones with same target sequence:
(none)
