Construct: sgRNA BRDN0001148289
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAGGCATACACTGACGACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NEK8 (284086)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76942
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
28737863 |
- |
NEK8 |
NNNGCATACACTGACGACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
18457268 |
- |
CDKL5 |
NNNGCATGCAATGACGACAG |
NGG |
2 |
0.55 |
Tier II |
| 3 |
NC_000009.12 |
9 |
16430600 |
- |
BNC2 |
NNNGCATACATTAACGACAG |
NGG |
2 |
0.284 |
Tier II |
| 4 |
NC_000011.10 |
11 |
108568038 |
+ |
EXPH5 |
NNNGAATACACTGAAGACAG |
NGG |
2 |
0.127 |
Tier II |
| 5 |
NC_000009.12 |
9 |
138037176 |
- |
CACNA1B |
NNNGAATACACTGAGGACAG |
NGG |
2 |
0.0286 |
Tier II |
| 6 |
NC_000002.12 |
2 |
1930968 |
+ |
MYT1L |
NNNGCATACACTGACTAGAG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000012.12 |
12 |
61701489 |
+ |
LOC105369793 |
NNNGCATACACTGACAACAG |
NGA |
2 |
0.0694 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
43665417 |
+ |
Cc2d2a |
NNNGCCAACACTGACGACAG |
NGG |
2 |
0.3977 |
Tier II |
| 2 |
NC_000070.6 |
4 |
149027370 |
+ |
Pex14 |
NNNTCATGCACTGACGACAG |
NGG |
2 |
0.2667 |
Tier II |
| 3 |
NC_000071.6 |
5 |
18348271 |
+ |
Gnai1 |
NNNGCATACACTGAAGACAG |
NGG |
1 |
0.2222 |
Tier II |
| 4 |
NC_000073.6 |
7 |
73531721 |
+ |
Chd2 |
NNNGCAGACAGTGACGACAG |
NGG |
2 |
0.1471 |
Tier II |
| 5 |
NC_000080.6 |
14 |
39442979 |
- |
Nrg3 |
NNNACATACACTGATGACAG |
NGG |
2 |
0.06 |
Tier II |
| 6 |
NC_000080.6 |
14 |
39442979 |
- |
Gm31606 |
NNNACATACACTGATGACAG |
NGG |
2 |
0.06 |
Tier II |
| 7 |
NC_000072.6 |
6 |
91169513 |
- |
Hdac11 |
NNNGCACACACTGAGGACAG |
NGG |
2 |
0.0344 |
Tier II |
| 8 |
NC_000077.6 |
11 |
84207354 |
- |
Acaca |
NNNGCATACACTGAGGTCAG |
NGG |
2 |
0.0067 |
Tier II |
| 9 |
NC_000068.7 |
2 |
155975938 |
- |
Cep250 |
NNNGCATACACTGAGGACAG |
NGA |
2 |
0.0035 |
Tier II |
| 10 |
NC_000069.6 |
3 |
141638634 |
- |
Unc5c |
NNNGCATTCACTGACCACAG |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
