Construct: sgRNA BRDN0001148290
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATTGGTGAAGTTATAACAGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CCL2 (6347)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77867
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
34256249 |
- |
CCL2 |
NNNGGTGAAGTTATAACAGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
37941162 |
+ |
TMCO5A |
NNNGGTTAAGTTACAACAGC |
NGG |
2 |
0.1633 |
Tier I |
| 3 |
NC_000014.9 |
14 |
91285013 |
- |
CCDC88C |
NNNGCTGAAGTTAAAACAGC |
NGG |
2 |
0.4864 |
Tier II |
| 4 |
NC_000013.11 |
13 |
43776404 |
+ |
ENOX1 |
NNNGGTGAAGTTATAAATGC |
NGG |
2 |
0.2333 |
Tier II |
| 5 |
NC_000001.11 |
1 |
217674727 |
+ |
SPATA17 |
NNNGGTGAAGTGTTAACAGC |
NGG |
2 |
0.15 |
Tier II |
| 6 |
NC_000001.11 |
1 |
50944492 |
- |
FAF1 |
NNNGGTGAAGTTATAGCTGC |
NGG |
2 |
0.0962 |
Tier II |
| 7 |
NC_000020.11 |
20 |
53389620 |
+ |
TSHZ2 |
NNNGGTGAAGTTACAACAGT |
NGG |
2 |
0.0857 |
Tier II |
| 8 |
NC_000018.10 |
18 |
33024554 |
- |
CCDC178 |
NNNGTTGAAGTTCTAACAGC |
NGG |
2 |
0.0632 |
Tier II |
| 9 |
NC_000013.11 |
13 |
108847982 |
+ |
MYO16 |
NNNGGTGAAGTTATGACAGC |
NTG |
2 |
0.0253 |
Tier II |
| 10 |
NC_000013.11 |
13 |
46045725 |
+ |
ZC3H13 |
NNNGGTGAATTTATAACAGC |
NGT |
2 |
0.0131 |
Tier II |
| 11 |
NC_000015.10 |
15 |
70741182 |
- |
UACA |
NNNGGTGAAGTGAGAACAGC |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000015.10 |
15 |
24081573 |
- |
PWRN4 |
NNNGGTGAAGCTATAAAAGC |
NGG |
2 |
0.3111 |
Tier III |
| 13 |
NC_000005.10 |
5 |
113913565 |
- |
LOC124901047 |
NNNGGTTCAGTTATAACAGC |
NGG |
2 |
0.2449 |
Tier III |
| 14 |
NC_000012.12 |
12 |
92473417 |
- |
LINC02397 |
NNNGCTGAAGTTATAACAGC |
NGA |
2 |
0.0546 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
97046974 |
+ |
Nars2 |
NNNGGTGAAGGTATGACAGC |
NGG |
2 |
0.26 |
Tier II |
| 2 |
NC_000076.6 |
10 |
69674782 |
+ |
Ank3 |
NNNGGTGAAGGTATGACAGC |
NGG |
2 |
0.26 |
Tier II |
| 3 |
NC_000071.6 |
5 |
48432224 |
+ |
Kcnip4 |
NNNGGTGAATTTATCACAGC |
NGG |
2 |
0.2216 |
Tier II |
| 4 |
NC_000068.7 |
2 |
156756780 |
+ |
Dlgap4 |
NNNGGTGAAGGGATAACAGC |
NGG |
2 |
0.2 |
Tier II |
| 5 |
NC_000068.7 |
2 |
169648155 |
- |
Tshz2 |
NNNAGTGAAGTTATAGCAGC |
NGG |
2 |
0.1731 |
Tier II |
| 6 |
NC_000075.6 |
9 |
16292644 |
- |
Fat3 |
NNNGGTGAGGTTATAACAGC |
NAG |
2 |
0.1728 |
Tier II |
| 7 |
NC_000071.6 |
5 |
48432224 |
+ |
Gm52772 |
NNNGGTGAATTTATCACAGC |
NGG |
2 |
0.2216 |
Tier III |
Other clones with same target sequence:
(none)
