Construct: sgRNA BRDN0001148292
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATGGATGATAGGACGAACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K13 (9175)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76906
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
185466880 |
- |
MAP3K13 |
NNNGGATGATAGGACGAACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
167991367 |
+ |
TENM2 |
NNNGGAACATAGGACGAACA |
NGG |
2 |
0.5385 |
Tier II |
| 3 |
NC_000004.12 |
4 |
150853979 |
+ |
LRBA |
NNNGGATGATAGGACAATCA |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000022.11 |
22 |
30246094 |
- |
LIF |
NNNGGATGCTGGGACGAACA |
NGG |
2 |
0.3714 |
Tier II |
| 5 |
NC_000018.10 |
18 |
49593457 |
+ |
LIPG |
NNNGGATGTTAGGAAGAACA |
NGG |
2 |
0.1333 |
Tier II |
| 6 |
NC_000006.12 |
6 |
10771303 |
+ |
MAK |
NNNGGATGGTAGGAGGAACA |
NGG |
2 |
0.0333 |
Tier II |
| 7 |
NC_000007.14 |
7 |
134033077 |
+ |
EXOC4 |
NNNGGATGATAGGAAGAAGA |
NGG |
2 |
0.0278 |
Tier II |
| 8 |
NC_000019.10 |
19 |
23323484 |
- |
ZNF91 |
NNNGGATGATAGGAAGAAGA |
NGG |
2 |
0.0278 |
Tier II |
| 9 |
NC_000006.12 |
6 |
55505230 |
- |
HMGCLL1 |
NNNGGATGATTGGAGGAACA |
NGG |
2 |
0.0154 |
Tier II |
| 10 |
NC_000010.11 |
10 |
112570951 |
- |
VTI1A |
NNNGGATGATAGGACTTACA |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000003.12 |
3 |
185466880 |
- |
LOC124906312 |
NNNGGATGATAGGACGAACA |
NGG |
0 |
1.0 |
Tier III |
| 12 |
NC_000013.11 |
13 |
109402368 |
+ |
LOC105370359 |
NNNGGATGAAAGGACGAACA |
NGA |
2 |
0.0595 |
Tier III |
| 13 |
NC_000016.10 |
16 |
55764346 |
- |
CES1P1 |
NNNGGATGAGAGGATGAACA |
NGG |
2 |
0.0333 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
21914206 |
- |
Map3k13 |
NNNGGATGATTGGACGGACA |
NGG |
2 |
0.0543 |
Tier I |
| 2 |
NC_000077.6 |
11 |
103594112 |
+ |
Gm884 |
NNNGGATTATAGGAAGAACA |
NGG |
2 |
0.1389 |
Tier II |
| 3 |
NC_000078.6 |
12 |
13366502 |
- |
Nbas |
NNNGGATGATAGGAAGAACA |
NGC |
2 |
0.0049 |
Tier II |
| 4 |
NC_000080.6 |
14 |
66861311 |
+ |
Dpysl2 |
NNNGGATGATAGGAACAACA |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000075.6 |
9 |
60128765 |
+ |
Thsd4 |
NNNGGATGATAGGACCAACC |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000080.6 |
14 |
59397396 |
+ |
Gm45935 |
NNNGGATTATAGGACTAACA |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000075.6 |
9 |
9605118 |
- |
Gm46102 |
NNNGGATGATAGGAAGAACA |
NGC |
2 |
0.0049 |
Tier III |
Other clones with same target sequence:
(none)
