Construct: sgRNA BRDN0001148297
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCACCAGTCAATATCCAGGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SIK3 (23387)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75755
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
116875867 |
- |
SIK3 |
NNNCCAGTCAATATCCAGGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
6021479 |
- |
IL2RA |
NNNCCAGTCAGTTTCCAGGT |
NGG |
2 |
0.195 |
Tier I |
| 3 |
NC_000002.12 |
2 |
241139424 |
+ |
PASK |
NNNCCTGTCAACATCCAGGT |
NGG |
2 |
0.6767 |
Tier II |
| 4 |
NC_000007.14 |
7 |
110882960 |
+ |
IMMP2L |
NNNCAAGCCAATATCCAGGT |
NGG |
2 |
0.5714 |
Tier II |
| 5 |
NC_000003.12 |
3 |
50451744 |
- |
CACNA2D2 |
NNNCCAGTCACCATCCAGGT |
NGG |
2 |
0.3789 |
Tier II |
| 6 |
NC_000017.11 |
17 |
34632035 |
+ |
TMEM132E |
NNNCAAGTCAGTATCCAGGT |
NGG |
2 |
0.3714 |
Tier II |
| 7 |
NC_000013.11 |
13 |
96286135 |
+ |
HS6ST3 |
NNNCCAGTCATTGTCCAGGT |
NGG |
2 |
0.2007 |
Tier II |
| 8 |
NC_000006.12 |
6 |
36484859 |
+ |
KCTD20 |
NNNCCAGTCAACATTCAGGT |
NGG |
2 |
0.0632 |
Tier II |
| 9 |
NC_000006.12 |
6 |
128308119 |
- |
PTPRK |
NNNCCAGTCAATATCCAGGT |
NTA |
2 |
0.0 |
Tier II |
| 10 |
NC_000013.11 |
13 |
40392763 |
- |
LINC00598 |
NNNCCATGCAATATCCAGGT |
NGG |
2 |
0.419 |
Tier III |
| 11 |
NC_000010.11 |
10 |
6021479 |
- |
LOC124902368 |
NNNCCAGTCAGTTTCCAGGT |
NGG |
2 |
0.195 |
Tier III |
| 12 |
NC_000010.11 |
10 |
79906138 |
+ |
BMS1P21 |
NNNCCAGTCAATATCTAGGT |
NTG |
2 |
0.012 |
Tier III |
| 13 |
NC_000001.11 |
1 |
205373950 |
- |
LEMD1-AS1 |
NNNCCAGTCAATATTCAGGG |
NGG |
2 |
0.0118 |
Tier III |
| 14 |
NC_000020.11 |
20 |
32599073 |
+ |
NOL4L-DT |
NNNCCAGTCAATAGCAAGGT |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
46195309 |
+ |
Sik3 |
NNNCCAGTCAATCTCCAGGT |
NGG |
1 |
0.2105 |
Tier I |
| 2 |
NC_000069.6 |
3 |
23882287 |
+ |
Naaladl2 |
NNNACAGTAAATATCCAGGT |
NGG |
2 |
0.7218 |
Tier II |
| 3 |
NC_000081.6 |
15 |
72592384 |
- |
Trappc9 |
NNNCCAGTCAATATCAAGGA |
NGG |
2 |
0.5625 |
Tier II |
| 4 |
NC_000072.6 |
6 |
37125635 |
+ |
Dgki |
NNNCCTGGCAATATCCAGGT |
NGG |
2 |
0.5238 |
Tier II |
| 5 |
NC_000072.6 |
6 |
88282143 |
+ |
Eefsec |
NNNTCAGTCAATCTCCAGGT |
NGG |
2 |
0.1684 |
Tier II |
| 6 |
NC_000073.6 |
7 |
80050113 |
- |
Zfp710 |
NNNCCAGTCAATACCCACGT |
NGG |
2 |
0.1361 |
Tier II |
| 7 |
NC_000080.6 |
14 |
122887984 |
- |
Pcca |
NNNCCATTCAATATCCAGGG |
NGG |
2 |
0.1008 |
Tier II |
| 8 |
NC_000080.6 |
14 |
49518442 |
- |
Slc35f4 |
NNNCCTGTCAATAGCCAGGT |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000084.6 |
18 |
89350641 |
- |
Dok6 |
NNNCCTGTCAATAGCCAGGT |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000069.6 |
3 |
111884980 |
+ |
Gm6602 |
NNNCCTGACAATATCCAGGT |
NGG |
2 |
0.5714 |
Tier III |
| 11 |
NC_000077.6 |
11 |
25350544 |
- |
4933427E13Rik |
NNNCCAGTCTTTATCCAGGT |
NGG |
2 |
0.2715 |
Tier III |
| 12 |
NC_000073.6 |
7 |
80050113 |
- |
Gm51449 |
NNNCCAGTCAATACCCACGT |
NGG |
2 |
0.1361 |
Tier III |
| 13 |
NC_000071.6 |
5 |
150002688 |
- |
Gm21048 |
NNNCCAGGCAATAGCCAGGT |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
