Construct: sgRNA BRDN0001148298
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGGTAAAAATAGAGCAGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PTK2B (2185)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76387
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
27420725 |
- |
PTK2B |
NNNGTAAAAATAGAGCAGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
185103758 |
+ |
C3orf70 |
NNNGCAAAAATAGAGCAGCC |
NGG |
2 |
0.4286 |
Tier II |
| 3 |
NC_000004.12 |
4 |
153708633 |
+ |
TLR2 |
NNNGCAAAAATAGAGCAGCC |
NGG |
2 |
0.4286 |
Tier II |
| 4 |
NC_000008.11 |
8 |
3704232 |
+ |
CSMD1 |
NNNGCAAAAATAGAGCAGCC |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000006.12 |
6 |
106294900 |
+ |
ATG5 |
NNNGTAAAAATAAAGCAGTG |
NGG |
2 |
0.426 |
Tier II |
| 6 |
NC_000007.14 |
7 |
95812628 |
+ |
DYNC1I1 |
NNNGTAAAAATAAAGCAGCC |
NGG |
2 |
0.3956 |
Tier II |
| 7 |
NC_000012.12 |
12 |
112693097 |
+ |
RPH3A |
NNNGAAAAAATAGAGCAGCT |
NGG |
2 |
0.35 |
Tier II |
| 8 |
NC_000011.10 |
11 |
114404228 |
+ |
RBM7 |
NNNGTGAAAATAGAGCAGTG |
NGG |
2 |
0.3297 |
Tier II |
| 9 |
NC_000002.12 |
2 |
224817355 |
- |
DOCK10 |
NNNGTAAAAATAGAGCAGTC |
NGG |
2 |
0.1978 |
Tier II |
| 10 |
NC_000002.12 |
2 |
164530490 |
+ |
GRB14 |
NNNGGAAAAATAGAGCAGCG |
NAG |
2 |
0.1659 |
Tier II |
| 11 |
NC_000015.10 |
15 |
32840386 |
- |
FMN1 |
NNNGGAAAAATAGAGCTGCG |
NGG |
2 |
0.0853 |
Tier II |
| 12 |
NC_000003.12 |
3 |
136736503 |
- |
STAG1 |
NNNGTAAAAATGGAGCAGCG |
NGA |
2 |
0.0502 |
Tier II |
| 13 |
NC_000002.12 |
2 |
144084068 |
+ |
GTDC1 |
NNNGTCAAAATAGAGCAGCG |
NGA |
2 |
0.0316 |
Tier II |
| 14 |
NC_000021.9 |
21 |
32381876 |
- |
URB1 |
NNNTTAAAAATAGAGCAGCG |
NGA |
2 |
0.0253 |
Tier II |
| 15 |
NC_000008.11 |
8 |
13508110 |
- |
DLC1 |
NNNGTAAAAATAGAGCACCG |
NTG |
2 |
0.0186 |
Tier II |
| 16 |
NC_000010.11 |
10 |
66538507 |
+ |
CTNNA3 |
NNNTTAAAAATAGAGCAGCG |
NTG |
2 |
0.0142 |
Tier II |
| 17 |
NC_000004.12 |
4 |
187976737 |
- |
LOC124900881 |
NNNGCAAAAATAGAGCAGCC |
NGG |
2 |
0.4286 |
Tier III |
| 18 |
NC_000011.10 |
11 |
21847852 |
+ |
LOC102723370 |
NNNGCAAAAATAGAGCAGCC |
NGG |
2 |
0.4286 |
Tier III |
| 19 |
NC_000011.10 |
11 |
129779699 |
- |
LOC124902793 |
NNNGCAAAAATAGAGCAGCC |
NGG |
2 |
0.4286 |
Tier III |
| 20 |
NC_000003.12 |
3 |
136736503 |
- |
DNAJC8P2 |
NNNGTAAAAATGGAGCAGCG |
NGA |
2 |
0.0502 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
109788026 |
- |
Faf1 |
NNNGTAAAAATAAAACAGCG |
NGG |
2 |
0.8688 |
Tier II |
| 2 |
NC_000073.6 |
7 |
37628084 |
+ |
Zfp536 |
NNNGCAAAAATAGAGCAGAG |
NGG |
2 |
0.4286 |
Tier II |
| 3 |
NC_000067.6 |
1 |
75413494 |
- |
Speg |
NNNGAAAAAATAGAGCAGCT |
NGG |
2 |
0.35 |
Tier II |
| 4 |
NC_000073.6 |
7 |
34229726 |
- |
Gpi1 |
NNNGTAAAAATAGAGCGGCG |
NAG |
2 |
0.0458 |
Tier II |
| 5 |
NC_000072.6 |
6 |
118891024 |
+ |
Cacna1c |
NNNGTAAAAATAGAGCAGGG |
NCG |
2 |
0.0134 |
Tier II |
| 6 |
NC_000072.6 |
6 |
6322072 |
+ |
Gm20619 |
NNNGTAAAAATAAAGCAGCT |
NGG |
2 |
0.6462 |
Tier III |
| 7 |
NC_000072.6 |
6 |
6322072 |
+ |
Gm34749 |
NNNGTAAAAATAAAGCAGCT |
NGG |
2 |
0.6462 |
Tier III |
Other clones with same target sequence:
(none)
