Construct: sgRNA BRDN0001148312
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCGAATATGATGTACCATGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIKFYVE (200576)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76893
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
208330564 |
- |
PIKFYVE |
NNNAATATGATGTACCATGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
18934566 |
+ |
HOMER3 |
NNNAAAATGATGTACCATGG |
NGG |
1 |
0.8667 |
Tier II |
| 3 |
NC_000001.11 |
1 |
243671484 |
+ |
AKT3 |
NNNAATATGATACACCATGG |
NGG |
2 |
0.7368 |
Tier II |
| 4 |
NC_000006.12 |
6 |
47967626 |
- |
PTCHD4 |
NNNAATATGATGTACCAAGG |
NGG |
1 |
0.6667 |
Tier II |
| 5 |
NC_000002.12 |
2 |
140767484 |
- |
LRP1B |
NNNAATATCATGTACCATGA |
NGG |
2 |
0.5048 |
Tier II |
| 6 |
NC_000006.12 |
6 |
165936285 |
+ |
PDE10A |
NNNAATGTGATGAACCATGG |
NGG |
2 |
0.4887 |
Tier II |
| 7 |
NC_000005.10 |
5 |
88242339 |
- |
TMEM161B |
NNNAATATGGTGTACCATGT |
NGG |
2 |
0.3889 |
Tier II |
| 8 |
NC_000004.12 |
4 |
65386380 |
+ |
EPHA5 |
NNNAATATCATGTACCATTG |
NGG |
2 |
0.359 |
Tier II |
| 9 |
NC_000004.12 |
4 |
36205261 |
+ |
ARAP2 |
NNNAATATGATCTTCCATGG |
NGG |
2 |
0.2824 |
Tier II |
| 10 |
NC_000008.11 |
8 |
55489983 |
- |
XKR4 |
NNNATTATAATGTACCATGG |
NGG |
2 |
0.2338 |
Tier II |
| 11 |
NC_000004.12 |
4 |
153213285 |
- |
TRIM2 |
NNNAATATAATGTACCCTGG |
NGG |
2 |
0.1134 |
Tier II |
| 12 |
NC_000001.11 |
1 |
48967739 |
+ |
AGBL4 |
NNNAATATGATGTACAATGG |
NGA |
2 |
0.0694 |
Tier II |
| 13 |
NC_000023.11 |
X |
135316472 |
+ |
ZNF75D |
NNNAATATAATGTACCATGG |
NGA |
2 |
0.0446 |
Tier II |
| 14 |
NC_000017.11 |
17 |
62719607 |
+ |
MARCHF10 |
NNNAATATGATGTGTCATGG |
NGG |
2 |
0.0311 |
Tier II |
| 15 |
NC_000023.11 |
X |
110280833 |
- |
AMMECR1 |
NNNTATATGATGTACCATGG |
NGC |
2 |
0.0141 |
Tier II |
| 16 |
NC_000006.12 |
6 |
165936285 |
+ |
LINC00473 |
NNNAATGTGATGAACCATGG |
NGG |
2 |
0.4887 |
Tier III |
| 17 |
NC_000001.11 |
1 |
70761017 |
+ |
LINC01788 |
NNNAATATGATCTACCATGG |
NGA |
2 |
0.0368 |
Tier III |
| 18 |
NC_000017.11 |
17 |
62719607 |
+ |
LOC105371855 |
NNNAATATGATGTGTCATGG |
NGG |
2 |
0.0311 |
Tier III |
| 19 |
NC_000002.12 |
2 |
78122939 |
+ |
LOC101927948 |
NNNCATATGATGTATCATGG |
NGG |
2 |
0.0235 |
Tier III |
| 20 |
NC_000002.12 |
2 |
78122939 |
+ |
LOC101927967 |
NNNCATATGATGTATCATGG |
NGG |
2 |
0.0235 |
Tier III |
| 21 |
NC_000008.11 |
8 |
37574268 |
- |
LINC01605 |
NNNAATATGATGTAGTATGG |
NGG |
2 |
0.0154 |
Tier III |
| 22 |
NC_000001.11 |
1 |
170524114 |
- |
GORAB-AS1 |
NNNAATATGATGTACCATGG |
NAT |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
65252453 |
- |
Pikfyve |
NNNAATGTGATGAACCATGG |
NGG |
2 |
0.4887 |
Tier I |
| 2 |
NC_000084.6 |
18 |
31315825 |
- |
Rit2 |
NNNAATATGATATACCATGA |
NGG |
2 |
0.875 |
Tier II |
| 3 |
NC_000069.6 |
3 |
123579314 |
+ |
Ndst3 |
NNNAATTTGATGTACCATGG |
NGG |
1 |
0.4375 |
Tier II |
| 4 |
NC_000068.7 |
2 |
54672655 |
+ |
Galnt13 |
NNNAACTTGATGTACCATGG |
NGG |
2 |
0.3977 |
Tier II |
| 5 |
NC_000077.6 |
11 |
26387783 |
- |
Fancl |
NNNAATTTGTTGTACCATGG |
NGG |
2 |
0.386 |
Tier II |
| 6 |
NC_000084.6 |
18 |
7387333 |
+ |
Mpp7 |
NNNAATATGTGGTACCATGG |
NGG |
2 |
0.3529 |
Tier II |
| 7 |
NC_000071.6 |
5 |
104954290 |
- |
Abcg3 |
NNNATTATGATGTACCATGT |
NGG |
2 |
0.2545 |
Tier II |
| 8 |
NC_000068.7 |
2 |
21806296 |
+ |
Gpr158 |
NNNAATATGATTTTCCATGG |
NGG |
2 |
0.2051 |
Tier II |
| 9 |
NC_000067.6 |
1 |
22480300 |
+ |
Rims1 |
NNNAATATGATATCCCATGG |
NGG |
2 |
0.2 |
Tier II |
| 10 |
NC_000075.6 |
9 |
53240723 |
+ |
Ddx10 |
NNNAACATGATGTACCATGG |
NGC |
2 |
0.0202 |
Tier II |
| 11 |
NC_000083.6 |
17 |
67068673 |
- |
Ptprm |
NNNAATATGAGGTACCATGG |
NTG |
2 |
0.0156 |
Tier II |
| 12 |
NC_000067.6 |
1 |
153737194 |
+ |
C230024C17Rik |
NNNAATGTGATGTACCATGG |
NGG |
1 |
0.7059 |
Tier III |
| 13 |
NC_000068.7 |
2 |
21806296 |
+ |
Gm39766 |
NNNAATATGATTTTCCATGG |
NGG |
2 |
0.2051 |
Tier III |
| 14 |
NC_000072.6 |
6 |
17165137 |
- |
Gm4876 |
NNNAATATGATGTACTATGG |
NGA |
2 |
0.0214 |
Tier III |
Other clones with same target sequence:
(none)
