Construct: sgRNA BRDN0001148321
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCAGCAACCTCTAACTAACC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DYRK1A (1859)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76754
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000021.9 |
21 |
37472876 |
+ |
DYRK1A |
NNNGCAACCTCTAACTAACC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
115978374 |
- |
SLC22A15 |
NNNGCAAACTCTAACTAACT |
NGG |
2 |
0.195 |
Tier II |
| 3 |
NC_000023.11 |
X |
106853439 |
+ |
TBC1D8B |
NNNGTAACCTCTAACTAACT |
NGG |
2 |
0.1909 |
Tier II |
| 4 |
NC_000003.12 |
3 |
17673401 |
+ |
TBC1D5 |
NNNGCAGCCTCTACCTAACC |
NGG |
2 |
0.1513 |
Tier II |
| 5 |
NC_000006.12 |
6 |
154824871 |
- |
SCAF8 |
NNNGCAACCTCTGCCTAACC |
NGG |
2 |
0.1398 |
Tier II |
| 6 |
NC_000010.11 |
10 |
15827470 |
- |
MINDY3 |
NNNGCAACCTCTGCCTAACC |
NGG |
2 |
0.1398 |
Tier II |
| 7 |
NC_000004.12 |
4 |
105342404 |
- |
TET2-AS1 |
NNNGCTACCTCTAACTAACC |
NGG |
1 |
0.7143 |
Tier III |
| 8 |
NC_000010.11 |
10 |
15827470 |
- |
LOC124902383 |
NNNGCAACCTCTGCCTAACC |
NGG |
2 |
0.1398 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
94659689 |
+ |
Dyrk1a |
NNNGCAACCTCTAACTAACC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000070.6 |
4 |
44078000 |
- |
Gne |
NNNGCAACCTCTGACTAATC |
NGG |
2 |
0.301 |
Tier II |
| 3 |
NC_000073.6 |
7 |
63463327 |
- |
Otud7a |
NNNGCAAGCTCTAACTAACC |
NAG |
2 |
0.1667 |
Tier II |
| 4 |
NC_000080.6 |
14 |
38781414 |
+ |
Nrg3 |
NNNGCGACCTCTACCTAACC |
NGG |
2 |
0.1531 |
Tier II |
| 5 |
NC_000072.6 |
6 |
18053161 |
+ |
Asz1 |
NNNGCAAGCTCTAACTAACC |
NTG |
2 |
0.025 |
Tier II |
| 6 |
NC_000086.7 |
X |
13238507 |
+ |
2010308F09Rik |
NNNTCAACCTCTAACTCACC |
NGG |
2 |
0.0642 |
Tier III |
| 7 |
NC_000086.7 |
X |
13238507 |
+ |
Gm16481 |
NNNTCAACCTCTAACTCACC |
NGG |
2 |
0.0642 |
Tier III |
Other clones with same target sequence:
(none)
