Construct: sgRNA BRDN0001148322
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATGAGGAGATCACACCCCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BCR (613)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76908
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000022.11 |
22 |
23273742 |
+ |
BCR |
NNNAGGAGATCACACCCCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
2831583 |
+ |
SH3BP2 |
NNNAGGCGATCACCCCCCGA |
NGG |
2 |
0.0938 |
Tier I |
| 3 |
NC_000016.10 |
16 |
1525435 |
- |
IFT140 |
NNNAGGAGCACACACCCCGA |
NGG |
2 |
0.4898 |
Tier II |
| 4 |
NC_000003.12 |
3 |
135146111 |
+ |
EPHB1 |
NNNTGGAGATCACACCCCAA |
NGG |
2 |
0.4545 |
Tier II |
| 5 |
NC_000001.11 |
1 |
177272313 |
- |
BRINP2 |
NNNAGGAGATCACACCCTGT |
NGG |
2 |
0.3857 |
Tier II |
| 6 |
NC_000002.12 |
2 |
235804793 |
- |
AGAP1 |
NNNAGCAGATCACACCCAGA |
NGG |
2 |
0.3671 |
Tier II |
| 7 |
NC_000019.10 |
19 |
18638046 |
- |
KLHL26 |
NNNAGGAGATCACACTCCTA |
NGG |
2 |
0.2051 |
Tier II |
| 8 |
NC_000010.11 |
10 |
58676344 |
+ |
BICC1 |
NNNAGCAGATCCCACCCCGA |
NGG |
2 |
0.1794 |
Tier II |
| 9 |
NC_000013.11 |
13 |
91906121 |
- |
GPC5 |
NNNAGGAGGTCACACCCCGA |
NGA |
2 |
0.0463 |
Tier II |
| 10 |
NC_000014.9 |
14 |
64847093 |
- |
SPTB |
NNNAGGAAATCACACCCCGA |
NGT |
2 |
0.0161 |
Tier II |
| 11 |
NC_000003.12 |
3 |
135146111 |
+ |
LOC102724019 |
NNNTGGAGATCACACCCCAA |
NGG |
2 |
0.4545 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
11711032 |
- |
Ptprg |
NNNAGGAGCTCACACCCCGA |
NGG |
1 |
0.5714 |
Tier II |
| 2 |
NC_000068.7 |
2 |
152007074 |
+ |
Slc52a3 |
NNNAGGATATCACACCCTGA |
NGG |
2 |
0.4018 |
Tier II |
| 3 |
NC_000076.6 |
10 |
77036756 |
+ |
Slc19a1 |
NNNAGGAGCTCACACTCCGA |
NGG |
2 |
0.1758 |
Tier II |
Other clones with same target sequence:
(none)
