Construct: sgRNA BRDN0001148324
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGAGCAGAAACCCTACGGGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PNCK (139728)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77329
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
153671331 |
- |
PNCK |
NNNGCAGAAACCCTACGGGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
57266826 |
+ |
R3HDM2 |
NNNGCAGAAACCCTACAGAA |
NGG |
2 |
0.6667 |
Tier I |
| 3 |
NC_000005.10 |
5 |
156229510 |
- |
SGCD |
NNNGCAGAAACCCTACAAGA |
NGG |
2 |
0.6462 |
Tier II |
| 4 |
NC_000011.10 |
11 |
46700397 |
+ |
ARHGAP1 |
NNNGCAGAAACCCTGCAGGA |
NGG |
2 |
0.6067 |
Tier II |
| 5 |
NC_000007.14 |
7 |
104904070 |
- |
LHFPL3 |
NNNGCAGAAGCCCTACGGAA |
NGG |
2 |
0.3968 |
Tier II |
| 6 |
NC_000002.12 |
2 |
73002052 |
- |
SFXN5 |
NNNGCAAAAATCCTACGGGA |
NGG |
2 |
0.3077 |
Tier II |
| 7 |
NC_000004.12 |
4 |
3144807 |
- |
HTT |
NNNGCAGAAACCCTAAGGGC |
NGG |
2 |
0.2273 |
Tier II |
| 8 |
NC_000020.11 |
20 |
51732910 |
+ |
ATP9A |
NNNGCAGAAACCTTACTGGA |
NGG |
2 |
0.175 |
Tier II |
| 9 |
NC_000017.11 |
17 |
81993726 |
- |
ASPSCR1 |
NNNGCAGCAACCCTCCGGGA |
NGG |
2 |
0.1169 |
Tier II |
| 10 |
NC_000007.14 |
7 |
154557742 |
- |
DPP6 |
NNNGCAGAAAGCCCACGGGA |
NGG |
2 |
0.0714 |
Tier II |
| 11 |
NC_000004.12 |
4 |
10028078 |
- |
SLC2A9 |
NNNGCAGAAACCCTCCTGGA |
NGG |
2 |
0.0682 |
Tier II |
| 12 |
NC_000023.11 |
X |
56055408 |
- |
KLF8 |
NNNGCAGAAACCCTACTGGA |
NAG |
2 |
0.0648 |
Tier II |
| 13 |
NC_000004.12 |
4 |
131386234 |
- |
LINC02377 |
NNNGCAGAAAACCTACAGGA |
NGG |
2 |
0.7 |
Tier III |
| 14 |
NC_000009.12 |
9 |
117777744 |
+ |
LOC105376244 |
NNNGCAGAAACCCTAAGTGA |
NGG |
2 |
0.6667 |
Tier III |
| 15 |
NC_000007.14 |
7 |
104904070 |
- |
LHFPL3-AS2 |
NNNGCAGAAGCCCTACGGAA |
NGG |
2 |
0.3968 |
Tier III |
| 16 |
NC_000005.10 |
5 |
113916920 |
- |
LOC124901047 |
NNNTCAGAAACCATACGGGA |
NGG |
2 |
0.1399 |
Tier III |
| 17 |
NC_000023.11 |
X |
56055408 |
- |
LOC124900486 |
NNNGCAGAAACCCTACTGGA |
NAG |
2 |
0.0648 |
Tier III |
| 18 |
NC_000006.12 |
6 |
119829075 |
- |
LOC105377975 |
NNNGCAGAAACCCTACAGGA |
NGC |
2 |
0.0207 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
73657484 |
- |
Pnck |
NNNGCAGAAACCCTACGGGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000069.6 |
3 |
27481248 |
+ |
Fndc3b |
NNNGAAGAAAACCTACGGGA |
NGG |
2 |
0.4286 |
Tier II |
| 3 |
NC_000071.6 |
5 |
23271776 |
- |
Lhfpl3 |
NNNGCAGAAACCCCACAGGA |
NGG |
2 |
0.2667 |
Tier II |
| 4 |
NC_000085.6 |
19 |
37281292 |
- |
Ide |
NNNGCAGAAACCCTGCGGGA |
NAG |
2 |
0.1685 |
Tier II |
| 5 |
NC_000084.6 |
18 |
6102651 |
- |
Arhgap12 |
NNNGCAGAAACCATAGGGGA |
NGG |
2 |
0.0592 |
Tier II |
| 6 |
NC_000074.6 |
8 |
124613228 |
- |
Capn9 |
NNNGCAGAAAGCCTTCGGGA |
NGG |
2 |
0.05 |
Tier II |
| 7 |
NC_000085.6 |
19 |
40731041 |
+ |
Entpd1 |
NNNGCAGAAACCGTACGGGA |
NAG |
2 |
0.0354 |
Tier II |
| 8 |
NC_000077.6 |
11 |
6653717 |
+ |
Gm11978 |
NNNGCAGAAACCTTACAGGA |
NGG |
2 |
0.6533 |
Tier III |
| 9 |
NC_000076.6 |
10 |
96232065 |
+ |
4930459C07Rik |
NNNCCTGAAACCCTACGGGA |
NGG |
2 |
0.3782 |
Tier III |
| 10 |
NC_000076.6 |
10 |
96232065 |
+ |
Gm40747 |
NNNCCTGAAACCCTACGGGA |
NGG |
2 |
0.3782 |
Tier III |
| 11 |
NC_000076.6 |
10 |
109638818 |
+ |
Gm40757 |
NNNGCAGAAGCCATACGGGA |
NGG |
2 |
0.2137 |
Tier III |
| 12 |
NC_000085.6 |
19 |
40731041 |
+ |
Gm41846 |
NNNGCAGAAACCGTACGGGA |
NAG |
2 |
0.0354 |
Tier III |
Other clones with same target sequence:
(none)
