Construct: sgRNA BRDN0001148325
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGCTCATGGGATACTCGCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PAK4 (10298)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77576
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
39173804 |
- |
PAK4 |
NNNTCATGGGATACTCGCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
72910247 |
- |
SLC39A11 |
NNNTCATGTGATACTCACTG |
NGG |
2 |
0.4978 |
Tier II |
| 3 |
NC_000007.14 |
7 |
131502395 |
+ |
PODXL |
NNNTCCTGGGAAACTCGCTG |
NGG |
2 |
0.3636 |
Tier II |
| 4 |
NC_000011.10 |
11 |
36423951 |
+ |
PRR5L |
NNNTCTTGGGCTACTCGCTG |
NGG |
2 |
0.2857 |
Tier II |
| 5 |
NC_000006.12 |
6 |
65458434 |
- |
EYS |
NNNTCATGGGATATTCCCTG |
NGG |
2 |
0.1725 |
Tier II |
| 6 |
NC_000013.11 |
13 |
114066482 |
+ |
RASA3 |
NNNTCATGGGATCCTCGGTG |
NGG |
2 |
0.0281 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
28564413 |
+ |
Pak4 |
NNNTCATGGGACACTCGTTG |
NGG |
2 |
0.609 |
Tier I |
| 2 |
NC_000070.6 |
4 |
113946090 |
+ |
Skint5 |
NNNTCATGGTATACTAGCTG |
NGG |
2 |
0.8125 |
Tier II |
| 3 |
NC_000086.7 |
X |
94135470 |
+ |
Zfx |
NNNTCGTGGGATACACGCTG |
NGG |
2 |
0.4135 |
Tier II |
| 4 |
NC_000070.6 |
4 |
108020988 |
- |
Podn |
NNNTCATTGGATACACGCTG |
NGG |
2 |
0.3618 |
Tier II |
| 5 |
NC_000068.7 |
2 |
44870293 |
- |
Gtdc1 |
NNNTCATGGGATACTTGCTG |
NGG |
1 |
0.3077 |
Tier II |
| 6 |
NC_000079.6 |
13 |
74152233 |
- |
Slc9a3 |
NNNTCATGGGATACTTGCTG |
NGG |
1 |
0.3077 |
Tier II |
| 7 |
NC_000080.6 |
14 |
8627621 |
- |
Cfap20dc |
NNNTCATGGGATACTTGCTT |
NGG |
2 |
0.2154 |
Tier II |
| 8 |
NC_000072.6 |
6 |
49122155 |
- |
Malsu1 |
NNNCCATGGGATACTTGCTG |
NGG |
2 |
0.1991 |
Tier II |
| 9 |
NC_000072.6 |
6 |
49122155 |
- |
Igf2bp3 |
NNNCCATGGGATACTTGCTG |
NGG |
2 |
0.1991 |
Tier II |
| 10 |
NC_000073.6 |
7 |
36632359 |
- |
Gm36722 |
NNNCCATGGGATACTTGCTG |
NGG |
2 |
0.1991 |
Tier II |
| 11 |
NC_000085.6 |
19 |
7800314 |
+ |
Slc22a26 |
NNNCCATGGGATACTTGCTG |
NGG |
2 |
0.1991 |
Tier II |
| 12 |
NC_000076.6 |
10 |
40402784 |
- |
Cdk19 |
NNNTCATGGGATACATGCTG |
NGG |
2 |
0.1781 |
Tier II |
| 13 |
NC_000068.7 |
2 |
51956215 |
+ |
Nmi |
NNNTCATGGGATACTGGCTG |
NGG |
1 |
0.1538 |
Tier II |
| 14 |
NC_000085.6 |
19 |
42071203 |
- |
Hoga1 |
NNNTCATGGGATACTGGCTG |
NGG |
1 |
0.1538 |
Tier II |
| 15 |
NC_000069.6 |
3 |
67385935 |
- |
Mlf1 |
NNNTCATGGGATACCTGCTG |
NGG |
2 |
0.0839 |
Tier II |
| 16 |
NC_000085.6 |
19 |
25535244 |
+ |
Dmrt1 |
NNNTCATGGGATACCTGCTG |
NGG |
2 |
0.0839 |
Tier II |
| 17 |
NC_000074.6 |
8 |
85747638 |
+ |
Itfg1 |
NNNTCATGGGATACTTGGTG |
NGG |
2 |
0.041 |
Tier II |
| 18 |
NC_000086.7 |
X |
94135470 |
+ |
Gm46691 |
NNNTCGTGGGATACACGCTG |
NGG |
2 |
0.4135 |
Tier III |
| 19 |
NC_000081.6 |
15 |
18337017 |
+ |
4921515E04Rik |
NNNCCATGGGATACTTGCTG |
NGG |
2 |
0.1991 |
Tier III |
| 20 |
NC_000071.6 |
5 |
28059584 |
+ |
Gm35223 |
NNNTCATGGGATACATGCTG |
NGG |
2 |
0.1781 |
Tier III |
| 21 |
NC_000074.6 |
8 |
85747638 |
+ |
Gm39214 |
NNNTCATGGGATACTTGGTG |
NGG |
2 |
0.041 |
Tier III |
Other clones with same target sequence:
(none)
