Construct: sgRNA BRDN0001148328

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATTATGCTTGGCTTTCACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRPF4B (8899)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76429

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 4043982 - PRP4K NNNATGCTTGGCTTTCACTG NGG 0 1.0 Tier I
2 NC_000004.12 4 76739510 - SHROOM3 NNNATGCTTGGGCTTCACTG NGG 2 0.3509 Tier I
3 NC_000023.11 X 38347920 + OTC NNNATGCTTGGATTTCACTA NGG 2 0.6696 Tier II
4 NC_000015.10 15 71638153 - THSD4 NNNATTCTTGACTTTCACTG NGG 2 0.6667 Tier II
5 NC_000005.10 5 22829565 + CDH12 NNNAGGCTTGGCTTTAACTG NGG 2 0.64 Tier II
6 NC_000011.10 11 70400821 + CTTN NNNAGGCTTGGCTTTCACTG NGG 1 0.64 Tier II
7 NC_000001.11 1 89844766 - LRRC8D NNNAGGCTAGGCTTTCACTG NGG 2 0.5943 Tier II
8 NC_000009.12 9 95088555 + AOPEP NNNGTGTTTGGCTTTCACTG NGG 2 0.5078 Tier II
9 NC_000015.10 15 66245068 + MEGF11 NNNATGCTTGGATATCACTG NGG 2 0.4422 Tier II
10 NC_000003.12 3 65368585 - MAGI1 NNNTTGCTTGGCTTTCAATG NGG 2 0.3427 Tier II
11 NC_000006.12 6 1903937 - GMDS NNNTAGCTTGGCTTTCACTG NGG 2 0.3182 Tier II
12 NC_000005.10 5 70056907 + SMN2 NNNATGCTTGCATTTCACTG NGG 2 0.3061 Tier II
13 NC_000005.10 5 70932331 + SMN1 NNNATGCTTGCATTTCACTG NGG 2 0.3061 Tier II
14 NC_000012.12 12 122604828 + KNTC1 NNNATGCTTGGCTTCAACTG NGG 2 0.2727 Tier II
15 NC_000018.10 18 21629299 - SNRPD1 NNNATGCTTAGCTCTCACTG NGG 2 0.2667 Tier II
16 NC_000015.10 15 88638224 - ISG20 NNNGTGCTTGTCTTTCACTG NGG 2 0.2404 Tier II
17 NC_000001.11 1 180290361 + ACBD6 NNNATGCTTGGCTCTCACTT NGG 2 0.2 Tier II
18 NC_000002.12 2 161878348 + SLC4A10 NNNCTGCTTGTCTTTCACTG NGG 2 0.1357 Tier II
19 NC_000011.10 11 78351668 - GAB2 NNNATGCTTGGCTTTCAGTA NGG 2 0.125 Tier II
20 NC_000011.10 11 16311076 + SOX6 NNNATGATTGGCTTTCAGTG NGG 2 0.1 Tier II
21 NC_000001.11 1 2464315 + PLCH2 NNNATCCTTGGCTTTCTCTG NGG 2 0.0909 Tier II
22 NC_000003.12 3 51029409 + DOCK3 NNNGTGCTTGGCTTTCAGTG NGG 2 0.0833 Tier II
23 NC_000005.10 5 60060575 + PDE4D NNNATGCTTGCCTTTCCCTG NGG 2 0.0756 Tier II
24 NC_000003.12 3 42384726 - LYZL4 NNNATGTTTGGCTTTCACTG NGA 2 0.0564 Tier II
25 NC_000005.10 5 160777715 - ATP10B NNNATACTTGGCTTTCACTG NTG 2 0.039 Tier II
26 NC_000002.12 2 32680691 + TTC27 NNNATGCTTGGCTTTCTCTG NTG 2 0.0052 Tier II
27 NC_000017.11 17 49779396 + FAM117A NNNATGCCTGGCTGTCACTG NGG 2 0.0 Tier II
28 NC_000001.11 1 170067742 - KIFAP3 NNNATGCTTGGCTTTCACTG NTT 2 0.0 Tier II
29 NC_000009.12 9 114847399 - LOC645266 NNNGTGCTTGGCTTTCACTG NGG 1 0.625 Tier III
30 NC_000005.10 5 125860707 - LOC124901056 NNNGTGATTGGCTTTCACTG NGG 2 0.4688 Tier III
31 NC_000023.11 X 22322305 - PTCHD1-AS NNNATGGTTAGCTTTCACTG NGG 2 0.4392 Tier III
32 NC_000004.12 4 76739510 - SHROOM3-AS1 NNNATGCTTGGGCTTCACTG NGG 2 0.3509 Tier III
33 NC_000010.11 10 70945959 + LINC02622 NNNATGATTGCCTTTCACTG NGG 2 0.3214 Tier III
34 NC_000005.10 5 70932331 + SMN-AS1 NNNATGCTTGCATTTCACTG NGG 2 0.3061 Tier III
35 NC_000003.12 3 178203907 - LOC105374235 NNNATCCTTGGCTTTTACTG NGG 2 0.2098 Tier III
36 NC_000001.11 1 60156181 - LINC02778 NNNATGCTGGGCTTTTACTG NGG 2 0.1905 Tier III
37 NC_000001.11 1 60156181 - LOC107984963 NNNATGCTGGGCTTTTACTG NGG 2 0.1905 Tier III
38 NC_000006.12 6 4467768 - LOC105374894 NNNAAGCTTGGCTTTCACCG NGG 2 0.1379 Tier III
39 NC_000011.10 11 41822966 - LINC01499 NNNATGCCTGGCTTTCTCTG NGG 2 0.1333 Tier III
40 NC_000011.10 11 34793002 + LOC102723568 NNNATGCTTGGCTTTCACCC NGG 2 0.1182 Tier III
41 NC_000002.12 2 67108450 - LINC01828 NNNATGGTTGGCTTTCTCTG NGG 2 0.0627 Tier III
42 NC_000003.12 3 149462768 - FKBP1AP4 NNNATGCTTGGCTCTCACTG NTG 2 0.0111 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 92940729 - Shroom3 NNNATGCTTAGATTTCACTG NGG 2 0.6667 Tier I
2 NC_000079.6 13 34890664 - Prpf4b NNNATGCTTGGCTTTTACTG NGG 1 0.3077 Tier I
3 NC_000077.6 11 33676331 - Kcnip1 NNNATACTCGGCTTTCACTG NGG 2 0.9231 Tier II
4 NC_000080.6 14 65012915 - Ints9 NNNATGTTTAGCTTTCACTG NGG 2 0.7583 Tier II
5 NC_000080.6 14 50814764 + Parp2 NNNATGCTATGCTTTCACTG NGG 2 0.7545 Tier II
6 NC_000074.6 8 84839049 - Rad23a NNNATGCCTGGATTTCACTG NGG 2 0.7143 Tier II
7 NC_000070.6 4 156238887 + Noc2l NNNATGCTTGGCTTTCACTT NGG 1 0.7 Tier II
8 NC_000073.6 7 117421826 - Xylt1 NNNATGCTTGGCTTTCACTT NGG 1 0.7 Tier II
9 NC_000071.6 5 125065031 - Ncor2 NNNATGAGTGGCTTTCACTG NGG 2 0.55 Tier II
10 NC_000073.6 7 49575925 + Nav2 NNNAGGCTTTGCTTTCACTG NGG 2 0.52 Tier II
11 NC_000080.6 14 19949596 + Gng2 NNNATCCTTGGCATTCACTG NGG 2 0.472 Tier II
12 NC_000074.6 8 110855320 - Cog4 NNNATGCTAGGCTTTCACTC NGG 2 0.398 Tier II
13 NC_000077.6 11 104790412 + Gm11639 NNNATGCTCGCCTTTCACTG NGG 2 0.3956 Tier II
14 NC_000070.6 4 152030921 - Tas1r1 NNNATGCCTGTCTTTCACTG NGG 2 0.3846 Tier II
15 NC_000077.6 11 62693990 - Zfp287 NNNATGCTGGGCTTACACTG NGG 2 0.3584 Tier II
16 NC_000083.6 17 86496559 - Prkce NNNATGGTTGGATTTCACTG NGG 2 0.3361 Tier II
17 NC_000068.7 2 163208768 + Tox2 NNNATGCCTGGCTTTTACTG NGG 2 0.3077 Tier II
18 NC_000068.7 2 34788993 + Rabepk NNNATGCTTGCCTTTCACTT NGG 2 0.3 Tier II
19 NC_000079.6 13 13808651 - Gng4 NNNATGCTTGCCATTCACTG NGG 2 0.2967 Tier II
20 NC_000071.6 5 24403830 - Abcb8 NNNGTGCTTGGGTTTCACTG NGG 2 0.2778 Tier II
21 NC_000084.6 18 60819823 + Tcof1 NNNATGCTGGCCTTTCACTG NGG 2 0.2653 Tier II
22 NC_000068.7 2 38575003 + Nek6 NNNATGCCTGGCTTTCACTG NAG 2 0.2593 Tier II
23 NC_000071.6 5 112366735 + Hps4 NNNATGTTTGGCTCTCACTG NGG 2 0.2321 Tier II
24 NC_000071.6 5 119852637 - Tbx5 NNNCTGCTTGGCTTTCATTG NGG 2 0.2269 Tier II
25 NC_000072.6 6 113059430 + Thumpd3 NNNATGCTTGGCTTTTACTT NGG 2 0.2154 Tier II
26 NC_000068.7 2 139744694 + Ism1 NNNATGCTTGGCATTCACAG NGG 2 0.1978 Tier II
27 NC_000080.6 14 54571737 - Ajuba NNNAGGCTTGGCTTTCACAG NGG 2 0.1829 Tier II
28 NC_000070.6 4 57683726 - Pakap NNNGTGCTTGGCTCTCACTG NGG 2 0.1786 Tier II
29 NC_000082.6 16 34757173 + Mylk NNNATGCTTCCCTTTCACTG NGG 2 0.1714 Tier II
30 NC_000067.6 1 63485126 + Adam23 NNNATGCTTAGCTTTCCCTG NGG 2 0.1647 Tier II
31 NC_000076.6 10 32451278 - Nkain2 NNNATGCTTCTCTTTCACTG NGG 2 0.1538 Tier II
32 NC_000077.6 11 22268577 - Ehbp1 NNNATGCTTCTCTTTCACTG NGG 2 0.1538 Tier II
33 NC_000071.6 5 120147160 + Rbm19 NNNATGCTTGGTTTTCACAG NGG 2 0.1538 Tier II
34 NC_000079.6 13 57650437 - Spock1 NNNATGCATGGCTTTCCCTG NGG 2 0.1412 Tier II
35 NC_000069.6 3 57824356 + Rnf13 NNNATGCTTGGTGTTCACTG NGG 2 0.1405 Tier II
36 NC_000077.6 11 7115255 - Adcy1 NNNATGCGTGGCTTTCCCTG NGG 2 0.1294 Tier II
37 NC_000072.6 6 96841203 + Tafa4 NNNCTGCTTGGCTTTTACTG NGG 2 0.1086 Tier II
38 NC_000067.6 1 89596624 + Agap1 NNNAAGCTTGGCTTTGACTG NGG 2 0.0769 Tier II
39 NC_000084.6 18 33055399 - Camk4 NNNATGCTTAGCTTTCACTG NGA 2 0.0648 Tier II
40 NC_000079.6 13 114029498 + Arl15 NNNATGCTTGGCTTTCCCAG NGG 2 0.0504 Tier II
41 NC_000071.6 5 140495529 + Gm4869 NNNGTGCTTGGCTTTCACTG NGA 2 0.0434 Tier II
42 NC_000075.6 9 34799538 + Kirrel3 NNNATGCTTGGCTTGCACAG NGG 2 0.0143 Tier II
43 NC_000074.6 8 46088269 + Snx25 NNNATGCTTGGCTTCCACTG NTG 2 0.0106 Tier II
44 NC_000068.7 2 4445821 - Frmd4a NNNATGCTTGGCTTGCTCTG NGG 2 0.0067 Tier II
45 NC_000069.6 3 105612289 + Kcnd3 NNNATGCTTGGCTGTCACTG NGA 2 0.0 Tier II
46 NC_000077.6 11 33676331 - Gm12119 NNNATACTCGGCTTTCACTG NGG 2 0.9231 Tier III
47 NC_000075.6 9 41416528 + Mir100hg NNNATGCTTGGCTTTTAATG NGG 2 0.1657 Tier III
48 NC_000079.6 13 48710834 + Gm36784 NNNATGCTTGGCTTTCACGC NGG 2 0.1071 Tier III
49 NC_000079.6 13 48711817 - Gm36784 NNNATGCTTGGCTTTCACGC NGG 2 0.1071 Tier III
50 NC_000069.6 3 144048975 - Gm40161 NNNATGCTTGGCCTTCTCTG NGG 2 0.1053 Tier III
51 NC_000069.6 3 51511076 - Gm36008 NNNATCCTTGGCTTTCTCTG NGG 2 0.0909 Tier III
52 NC_000078.6 12 27205196 - Gm40872 NNNATGCTTGGCTTCTACTG NGG 2 0.0839 Tier III
53 NC_000076.6 10 17624990 - Gm32543 NNNATGCTTGGGTTTCACTG NGT 2 0.0072 Tier III
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Other clones with same target sequence:

(none)