Construct: sgRNA BRDN0001148329
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAAAGGATCAATAGACAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NEK5 (341676)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76119
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
52112296 |
- |
NEK5 |
NNNAGGATCAATAGACAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
35700636 |
- |
SPEF2 |
NNNAGGATCAATAGCCAATG |
NGG |
2 |
0.1259 |
Tier I |
| 3 |
NC_000002.12 |
2 |
63394873 |
+ |
WDPCP |
NNNAGGAACAATAGACAACA |
NGG |
2 |
0.75 |
Tier II |
| 4 |
NC_000007.14 |
7 |
43321962 |
+ |
HECW1 |
NNNAGAATGAATAGACAACG |
NGG |
2 |
0.619 |
Tier II |
| 5 |
NC_000012.12 |
12 |
27354404 |
+ |
BMAL2 |
NNNAGGAACAATAGACATCG |
NGG |
2 |
0.4 |
Tier II |
| 6 |
NC_000020.11 |
20 |
13519163 |
+ |
TASP1 |
NNNAGGAACAATAGACAATG |
NGG |
2 |
0.3692 |
Tier II |
| 7 |
NC_000006.12 |
6 |
49447483 |
- |
MMUT |
NNNAGGATAAATAGACAACC |
NGG |
2 |
0.3673 |
Tier II |
| 8 |
NC_000015.10 |
15 |
55929513 |
- |
NEDD4 |
NNNAGGAACAATAGACACCG |
NGG |
2 |
0.1524 |
Tier II |
| 9 |
NC_000011.10 |
11 |
39723180 |
+ |
LOC105376637 |
NNNAGGAACAATAGACAATG |
NGG |
2 |
0.3692 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
48619407 |
+ |
Kcnip4 |
NNNAGGATCAATAAACAACA |
NGG |
2 |
0.7031 |
Tier II |
| 2 |
NC_000074.6 |
8 |
37857172 |
- |
Sgcz |
NNNAGAATCAATAGACAACC |
NGG |
2 |
0.4286 |
Tier II |
| 3 |
NC_000080.6 |
14 |
70655164 |
- |
Npm2 |
NNNAGGATCAATAGACAACA |
NAG |
2 |
0.2431 |
Tier II |
| 4 |
NC_000072.6 |
6 |
83496356 |
- |
Dguok |
NNNAGGAGCAATAGTCAACG |
NGG |
2 |
0.1467 |
Tier II |
| 5 |
NC_000072.6 |
6 |
116105439 |
+ |
Tmcc1 |
NNNAGGATCAATAGACATGG |
NGG |
2 |
0.0625 |
Tier II |
| 6 |
NC_000079.6 |
13 |
48159309 |
- |
A330033J07Rik |
NNNGGGATCAATAGACAACC |
NGG |
2 |
0.2679 |
Tier III |
Other clones with same target sequence:
(none)
