Construct: sgRNA BRDN0001148332
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTTCGCTCAAAGAACACAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TRIB2 (28951)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75604
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
12723372 |
- |
TRIB2 |
NNNTCGCTCAAAGAACACAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
40509051 |
+ |
SUGCT |
NNNTCTCCCAAAGAACACAT |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000002.12 |
2 |
153089485 |
- |
GALNT13 |
NNNAAGCTCAAAGAACACAT |
NGG |
2 |
0.2721 |
Tier II |
| 4 |
NC_000001.11 |
1 |
219261858 |
- |
LYPLAL1 |
NNNGCGCTCAAAGAACACCT |
NGG |
2 |
0.1293 |
Tier II |
| 5 |
NC_000019.10 |
19 |
31169766 |
- |
TSHZ3 |
NNNTCGCTCAAAGGACACAT |
NGC |
2 |
0.0104 |
Tier II |
| 6 |
NC_000008.11 |
8 |
37730693 |
+ |
LOC101929622 |
NNNTCCATCAAAGAACACAT |
NGG |
2 |
0.5114 |
Tier III |
| 7 |
NC_000014.9 |
14 |
28778660 |
+ |
LINC01551 |
NNNTCTCTCAAAGAACAAAT |
NGG |
2 |
0.359 |
Tier III |
| 8 |
NC_000003.12 |
3 |
34247920 |
+ |
LINC01811 |
NNNTTGCTCAAAGAACACAA |
NGG |
2 |
0.358 |
Tier III |
| 9 |
NC_000001.11 |
1 |
31476321 |
- |
LOC105379772 |
NNNAAGCTCAAAGAACACAT |
NGG |
2 |
0.2721 |
Tier III |
| 10 |
NC_000001.11 |
1 |
171085102 |
- |
LOC646804 |
NNNTCCCTCAAAGAACACAT |
NAG |
2 |
0.1768 |
Tier III |
| 11 |
NC_000011.10 |
11 |
130783615 |
- |
LOC105369576 |
NNNTTGCTCAAAGAACACAT |
NAG |
2 |
0.165 |
Tier III |
| 12 |
NC_000021.9 |
21 |
17218305 |
+ |
LOC107985511 |
NNNTAGCTCAAAGAACACCT |
NGG |
2 |
0.1182 |
Tier III |
| 13 |
NC_000019.10 |
19 |
31169766 |
- |
LINC01791 |
NNNTCGCTCAAAGGACACAT |
NGC |
2 |
0.0104 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
15809877 |
+ |
Trib2 |
NNNTCGCTCAAAGAACACAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000069.6 |
3 |
59263224 |
- |
Med12l |
NNNTCGATCAAAGAACCCAT |
NGG |
2 |
0.1324 |
Tier I |
| 3 |
NC_000068.7 |
2 |
140503575 |
- |
Macrod2 |
NNNTCACACAAAGAACACAT |
NGG |
2 |
0.8 |
Tier II |
| 4 |
NC_000077.6 |
11 |
109690388 |
+ |
Fam20a |
NNNTAGCTCAAAGAAAACAT |
NGG |
2 |
0.5714 |
Tier II |
| 5 |
NC_000075.6 |
9 |
23349828 |
- |
Bmper |
NNNTGGCACAAAGAACACAT |
NGG |
2 |
0.48 |
Tier II |
| 6 |
NC_000079.6 |
13 |
24924314 |
- |
Aldh5a1 |
NNNGCCCTCAAAGAACACAT |
NGG |
2 |
0.4261 |
Tier II |
| 7 |
NC_000071.6 |
5 |
23632677 |
- |
Srpk2 |
NNNTGGCTCAGAGAACACAT |
NGG |
2 |
0.39 |
Tier II |
| 8 |
NC_000071.6 |
5 |
130936784 |
- |
Galnt17 |
NNNTCCCTCAAAGAATACAT |
NGG |
2 |
0.2098 |
Tier II |
| 9 |
NC_000073.6 |
7 |
133810671 |
+ |
Fank1 |
NNNTGGCTCAAAGAAGACAT |
NGG |
2 |
0.0923 |
Tier II |
| 10 |
NC_000083.6 |
17 |
70254311 |
- |
Dlgap1 |
NNNTCGCTCAAAGAACACAA |
NGC |
2 |
0.0125 |
Tier II |
| 11 |
NC_000084.6 |
18 |
79303661 |
+ |
Gm2116 |
NNNTCGCTGAAAGAGCACAT |
NGG |
2 |
0.4024 |
Tier III |
Other clones with same target sequence:
(none)
