Construct: sgRNA BRDN0001148335

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGCCCCATAGGAAAACATCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIK3C2G (5288)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75740

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 18282480 + PIK3C2G NNNCCCATAGGAAAACATCA NGG 0 1.0 Tier I
2 NC_000007.14 7 11049788 - PHF14 NNNCCCATATGAAAACATCA NGG 1 0.8125 Tier II
3 NC_000009.12 9 5811843 + ERMP1 NNNTCCACAGGAAAACATCA NGG 2 0.8 Tier II
4 NC_000005.10 5 130104303 - CHSY3 NNNCCCATGAGAAAACATCA NGG 2 0.6222 Tier II
5 NC_000009.12 9 73158086 - ANXA1 NNNTCCATAGGAAAACATAA NGG 2 0.3429 Tier II
6 NC_000019.10 19 13796634 + ZSWIM4 NNNCCCATAGGAAAACAGCA NGG 1 0.3333 Tier II
7 NC_000018.10 18 63269545 + BCL2 NNNCCCATAGAAAAATATCA NGG 2 0.3077 Tier II
8 NC_000012.12 12 11117972 + PRH1 NNNCCCATAAGAAAACATCA NAG 2 0.242 Tier II
9 NC_000012.12 12 11117972 + PRH1-TAS2R14 NNNCCCATAAGAAAACATCA NAG 2 0.242 Tier II
10 NC_000011.10 11 7276421 - SYT9 NNNCACATAGGAAAACAGCA NGG 2 0.1905 Tier II
11 NC_000002.12 2 44720976 - CAMKMT NNNCCCATAGTAAAACATAA NGG 2 0.1648 Tier II
12 NC_000002.12 2 205697864 + NRP2 NNNCCCAGAGGAAAACCTCA NGG 2 0.1294 Tier II
13 NC_000003.12 3 29915417 - RBMS3 NNNCCCACAGGAAAACATGA NGG 2 0.125 Tier II
14 NC_000021.9 21 15742583 - USP25 NNNCCCATGGGAAAACTTCA NGG 2 0.0889 Tier II
15 NC_000007.14 7 110739511 + IMMP2L NNNCCCATAGGAAAATATCA NAG 2 0.0798 Tier II
16 NC_000008.11 8 105437700 - ZFPM2 NNNCCCATAGGAAAAGATTA NGG 2 0.071 Tier II
17 NC_000006.12 6 136912865 - PEX7 NNNGCCATAGGAAAACATGA NGG 2 0.0625 Tier II
18 NC_000001.11 1 15287038 + FHAD1 NNNCCCATAGGAAAAGGTCA NGG 2 0.0271 Tier II
19 NC_000012.12 12 1899659 + CACNA2D4 NNNCCCATAAGAAAACATCA NGC 2 0.0207 Tier II
20 NC_000006.12 6 151140862 - LOC124901432 NNNCCAATAGGAATACATCA NGG 2 0.4952 Tier III
21 NC_000002.12 2 139283722 + LOC105373643 NNNCCCACAGGAAAATATCA NGG 2 0.3077 Tier III
22 NC_000012.12 12 11117972 + PRH1-PRR4 NNNCCCATAAGAAAACATCA NAG 2 0.242 Tier III
23 NC_000005.10 5 98940298 - CHD1-DT NNNCCCATAGGACAACAACA NGG 2 0.1404 Tier III
24 NC_000012.12 12 115032532 + LOC102723639 NNNCACATAGGACAACATCA NGG 2 0.1203 Tier III
25 NC_000014.9 14 70213046 + LOC646548 NNNCCCCTAGGAAATCATCA NGG 2 0.0875 Tier III
26 NC_000014.9 14 70213046 + LOC107984686 NNNCCCCTAGGAAATCATCA NGG 2 0.0875 Tier III
27 NC_000004.12 4 94746437 + BMPR1B-DT NNNCCCATAGCAAAACATCA NGT 2 0.0069 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 26222336 - Qsox2 NNNTCCATATGAAAACATCA NGG 2 0.65 Tier I
2 NC_000080.6 14 96280209 - Klhl1 NNNCCCACAGGAAAACATAA NGG 2 0.4286 Tier I
3 NC_000072.6 6 13193119 + Vwde NNNCCCACAGGAAAACATCA NTG 2 0.039 Tier I
4 NC_000076.6 10 30673776 - Ncoa7 NNNCCAATAGGAAAACATCA NGG 1 0.9286 Tier II
5 NC_000078.6 12 102625009 + Itpk1 NNNCCAATAAGAAAACATCA NGG 2 0.8667 Tier II
6 NC_000068.7 2 20022440 - Etl4 NNNCCTAAAGGAAAACATCA NGG 2 0.7429 Tier II
7 NC_000072.6 6 142402764 - Golt1b NNNCCGACAGGAAAACATCA NGG 2 0.5 Tier II
8 NC_000072.6 6 48571272 - Rarres2 NNNCCCATATGAAAACATCT NGG 2 0.4875 Tier II
9 NC_000068.7 2 120752571 + Ttbk2 NNNCCCACAGCAAAACATCA NGG 2 0.4286 Tier II
10 NC_000077.6 11 110287231 - Abca5 NNNCCCACAGGAAAACATAA NGG 2 0.4286 Tier II
11 NC_000077.6 11 93304052 + Car10 NNNCCCATAGAAAAACACCA NGG 2 0.4286 Tier II
12 NC_000067.6 1 12767012 - Sulf1 NNNCTCATAGGAAAACAACA NGG 2 0.4242 Tier II
13 NC_000067.6 1 115788650 - Cntnap5a NNNCTCATAGGAAAACATCT NGG 2 0.3818 Tier II
14 NC_000075.6 9 8586559 - Trpc6 NNNCCCCTGGGAAAACATCA NGG 2 0.2917 Tier II
15 NC_000075.6 9 79988182 - Filip1 NNNCCAATAGGAAAACATCA NAG 2 0.2407 Tier II
16 NC_000084.6 18 58011498 - Fbn2 NNNCCCATAGCAAAACATTA NGG 2 0.1978 Tier II
17 NC_000076.6 10 120192524 + Irak3 NNNCCCATAGGAAAACACAA NGG 2 0.1837 Tier II
18 NC_000075.6 9 55156996 - Ube2q2 NNNCCTATAGGAAAACTTCA NGG 2 0.1238 Tier II
19 NC_000070.6 4 32419510 - Bach2 NNNCCCATAGCCAAACATCA NGG 2 0.1128 Tier II
20 NC_000077.6 11 3329823 - Pik3ip1 NNNGCCATAGGAAAACTTCA NGG 2 0.0667 Tier II
21 NC_000075.6 9 121474117 + Trak1 NNNCCCATAGGAAAAGATCA NAG 2 0.0399 Tier II
22 NC_000070.6 4 118221702 + Ptprf NNNCCCATAGGACAACCTCA NGG 2 0.0372 Tier II
23 NC_000086.7 X 53259733 - Fam122b NNNCCCATAGGAAAAGCTCA NGG 2 0.0271 Tier II
24 NC_000074.6 8 28921567 - Unc5d NNNCCCATAGGAAAACATGA NTG 2 0.0049 Tier II
25 NC_000085.6 19 20439242 + Gm41816 NNNCCTATAGGAAAATATCA NGG 2 0.2857 Tier III
26 NC_000083.6 17 31750163 - Gm30316 NNNCCCATAGCAAGACATCA NGG 2 0.2 Tier III
27 NC_000076.6 10 120192524 + Gm40781 NNNCCCATAGGAAAACACAA NGG 2 0.1837 Tier III
28 NC_000077.6 11 3329823 - Gm12592 NNNGCCATAGGAAAACTTCA NGG 2 0.0667 Tier III
29 NC_000073.6 7 119615522 - Gm39079 NNNCCCATAGGAAGACATCA NTG 2 0.0182 Tier III
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Other clones with same target sequence:

(none)