Construct: sgRNA BRDN0001148355
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTCGCTCATGACAGCCCAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NPR2 (4882)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77753
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
35801959 |
+ |
NPR2 |
NNNCGCTCATGACAGCCCAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000022.11 |
22 |
41580487 |
+ |
PMM1 |
NNNTGCTCATGACAGCCCAT |
NGG |
1 |
0.8 |
Tier II |
| 3 |
NC_000003.12 |
3 |
123493791 |
+ |
HACD2 |
NNNAGCTCATGACAGCCCAA |
NGG |
2 |
0.4737 |
Tier II |
| 4 |
NC_000019.10 |
19 |
18504768 |
- |
ELL |
NNNTTCTCATGACAGCCCAT |
NGG |
2 |
0.24 |
Tier II |
| 5 |
NC_000016.10 |
16 |
89922691 |
+ |
TUBB3 |
NNNCGCTCATGGCAGCCCCT |
NGG |
2 |
0.1494 |
Tier II |
| 6 |
NC_000012.12 |
12 |
123963057 |
- |
CCDC92 |
NNNCGCTCATGACAGCCCAA |
NGC |
2 |
0.0125 |
Tier II |
| 7 |
NC_000010.11 |
10 |
3658064 |
+ |
LOC105376360 |
NNNCGCTCATGAATGCCCAT |
NGG |
2 |
0.2051 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
43643368 |
+ |
Npr2 |
NNNCGCTCATGACAGCCCAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000081.6 |
15 |
8642917 |
- |
Slc1a3 |
NNNCGCTCATCACAGCCCTT |
NGG |
2 |
0.2308 |
Tier I |
| 3 |
NC_000070.6 |
4 |
107817256 |
+ |
Lrp8 |
NNNAGCTCATGACAGCCCTT |
NGG |
2 |
0.4534 |
Tier II |
| 4 |
NC_000068.7 |
2 |
117332270 |
+ |
Rasgrp1 |
NNNCGCTCATGACAGGCCAT |
NTG |
2 |
0.006 |
Tier II |
| 5 |
NC_000070.6 |
4 |
107817256 |
+ |
Lrp8os2 |
NNNAGCTCATGACAGCCCTT |
NGG |
2 |
0.4534 |
Tier III |
| 6 |
NC_000075.6 |
9 |
41434257 |
- |
Mir100hg |
NNNCACTCATGACAGTCCAT |
NGG |
2 |
0.2667 |
Tier III |
Other clones with same target sequence:
(none)
