Construct: sgRNA BRDN0001148358

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CATTGTGTGCATGAATAGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ETNK1 (55500)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75532

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 22659047 - ETNK1 NNNTGTGTGCATGAATAGCA NGG 0 1.0 Tier I
2 NC_000006.12 6 160442766 + SLC22A3 NNNTGTTTGCAGGAATAGCA NGG 2 0.2857 Tier I
3 NC_000014.9 14 50880445 - ABHD12B NNNTGTGTGCCTGAAGAGCA NGG 2 0.1385 Tier I
4 NC_000009.12 9 110326933 - TXNDC8 NNNTGTGTGCATGAGTAGCA NGG 1 0.65 Tier II
5 NC_000001.11 1 147668127 - ACP6 NNNTGTGTGCATGAAAATCA NGG 2 0.6061 Tier II
6 NC_000009.12 9 92986969 + FGD3 NNNTGAGTGCATGAATAACA NGG 2 0.6 Tier II
7 NC_000005.10 5 19592734 - CDH18 NNNTGTGTCTATGAATAGCA NGG 2 0.5068 Tier II
8 NC_000006.12 6 135973918 - PDE7B NNNTGGGGGCATGAATAGCA NGG 2 0.419 Tier II
9 NC_000018.10 18 76456826 - ZNF516 NNNTGTTTGCATGAATAGCT NGG 2 0.3429 Tier II
10 NC_000005.10 5 143153076 + ARHGAP26 NNNTGTCTGCATGAATAGAA NGG 2 0.2946 Tier II
11 NC_000007.14 7 154481016 + DPP6 NNNTGGGTGCATGAATACCA NGG 2 0.2721 Tier II
12 NC_000009.12 9 28376325 + LINGO2 NNNTGTGAGCTTGAATAGCA NGG 2 0.2462 Tier II
13 NC_000008.11 8 27364152 + PTK2B NNNTCTGTGCATGACTAGCA NGG 2 0.2143 Tier II
14 NC_000009.12 9 106963544 + ZNF462 NNNTGTGTGCCTGTATAGCA NGG 2 0.2133 Tier II
15 NC_000012.12 12 109441260 + MYO1H NNNTGTGTGCATGAATGGCA NGG 1 0.1765 Tier II
16 NC_000015.10 15 50138271 + ATP8B4 NNNTGTGTACATGATTAGCA NGG 2 0.1286 Tier II
17 NC_000001.11 1 27424400 + WASF2 NNNTTTGTGCATCAATAGCA NGG 2 0.1263 Tier II
18 NC_000005.10 5 21780578 + CDH12 NNNTGTGTGTATGAATAGGA NGG 2 0.1176 Tier II
19 NC_000003.12 3 127012529 + PLXNA1 NNNTGTGTGCAGGAATAGCC NGG 2 0.1136 Tier II
20 NC_000017.11 17 72423814 + LINC00673 NNNCGTGTGCATGAACAGCA NGG 2 0.4314 Tier III
21 NC_000001.11 1 219747681 - LOC105372926 NNNTGTGTGCATGTGTAGCA NGG 2 0.3467 Tier III
22 NC_000004.12 4 136991912 - LINC02511 NNNTGGGTGCATGAATAGCT NGG 2 0.3429 Tier III
23 NC_000001.11 1 1065408 + LOC100288175 NNNTGTGTGCAGGAGTAGCA NGG 2 0.325 Tier III
24 NC_000009.12 9 5614962 - LOC124902114 NNNTGTTTGCATGAATAGCA NGA 2 0.0397 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 143184606 - Etnk1 NNNTGTGCGCATGAATAGCA NGG 1 1.0 Tier I
2 NC_000078.6 12 70163371 - Abhd12b NNNTGTGTGCCTGAAGAGCA NGG 2 0.1385 Tier I
3 NC_000067.6 1 65288406 - Pth2r NNNTGTGTGAATGAAAAGCA NGG 2 0.7879 Tier II
4 NC_000067.6 1 9284067 - Sntg1 NNNTATGTGAATGAATAGCA NGG 2 0.7511 Tier II
5 NC_000081.6 15 66717027 + Tg NNNTGTGTGTGTGAATAGCA NGG 2 0.6118 Tier II
6 NC_000069.6 3 125598557 + Ndst4 NNNTGTCTGAATGAATAGCA NGG 2 0.5958 Tier II
7 NC_000073.6 7 42291834 + Vmn2r61 NNNTATGTGCATGAATATCA NGG 2 0.5778 Tier II
8 NC_000084.6 18 39458174 - Nr3c1 NNNTGTATGCATGGATAGCA NGG 2 0.4667 Tier II
9 NC_000085.6 19 34615192 + Ifit1bl1 NNNTGTGTGCATGAAAAGAA NGG 2 0.3896 Tier II
10 NC_000067.6 1 124119580 + Dpp10 NNNTATGTGCATGAATAGAA NGG 2 0.3714 Tier II
11 NC_000073.6 7 135024718 - Dock1 NNNTGTGTGCCTGAAAAGCA NGG 2 0.3636 Tier II
12 NC_000067.6 1 92061330 + Hdac4 NNNTGTGTGACTGAATAGCA NGG 2 0.3467 Tier II
13 NC_000079.6 13 100528238 + Ocln NNNTGTGTGCATGTGTAGCA NGG 2 0.3467 Tier II
14 NC_000079.6 13 100528810 - Ocln NNNTGTGTGCATGTGTAGCA NGG 2 0.3467 Tier II
15 NC_000070.6 4 18018575 - Mmp16 NNNTGTGTGCATGAATATAA NGG 2 0.2857 Tier II
16 NC_000078.6 12 118284769 - Sp4 NNNTGGGTGCATGAATAGAA NGG 2 0.2449 Tier II
17 NC_000079.6 13 89968203 + Xrcc4 NNNTGTGTGCATGAACAGCA NAG 2 0.1728 Tier II
18 NC_000067.6 1 118967919 + Gli2 NNNTGTGTGCATGTATAGCC NGG 2 0.1212 Tier II
19 NC_000086.7 X 20828685 - Araf NNNTGTGTGCATGCATACCA NGG 2 0.102 Tier II
20 NC_000085.6 19 3626224 + Lrp5 NNNTGTGTGCATGAATAGAC NGG 2 0.0974 Tier II
21 NC_000081.6 15 78563648 - Rac2 NNNTGTGTGCATGTATGGCA NGG 2 0.0941 Tier II
22 NC_000071.6 5 96439342 - Fras1 NNNTGTGTGCATGCAGAGCA NGG 2 0.0742 Tier II
23 NC_000083.6 17 70574724 + Dlgap1 NNNTGTGTGCATGACTAGCA NAG 2 0.0707 Tier II
24 NC_000072.6 6 115610098 - Mkrn2 NNNTGTGTGCATGAATGGCA NAG 2 0.0458 Tier II
25 NC_000068.7 2 79933879 + Pde1a NNNTGTGTACATGAATAGCA NGT 2 0.0104 Tier II
26 NC_000073.6 7 64211984 + Trpm1 NNNTGTGTGCATGATTAGCA NGT 2 0.0032 Tier II
27 NC_000080.6 14 47543319 + Atg14 NNNTGTGTGCATGAATAGCA NTC 2 0.0 Tier II
28 NC_000069.6 3 117101477 - Gm18430 NNNTGTGTGCCTGAATACCA NGG 2 0.1905 Tier III
29 NC_000079.6 13 115131889 + Gm41075 NNNTGTGTGCATGAATAGAA NGA 2 0.0298 Tier III
30 NC_000077.6 11 79185731 + Gm30413 NNNTGTGTGCATGAATAGCG NGT 2 0.0123 Tier III
31 NC_000085.6 19 33132372 - Gm29946 NNNTGTGTGCATGAATAGCA NAT 2 0.0 Tier III
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Other clones with same target sequence:

(none)