Construct: sgRNA BRDN0001148371
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TACCCATTGTAGTACATGGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MUSK (4593)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77500
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
110695467 |
- |
MUSK |
NNNCCATTGTAGTACATGGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
39016852 |
- |
PROSER1 |
NNNCCATTGTAGTACATAGT |
NAG |
2 |
0.1795 |
Tier II |
| 3 |
NC_000014.9 |
14 |
24908452 |
- |
STXBP6 |
NNNACATTGTAGTACATGGG |
NGG |
2 |
0.1486 |
Tier II |
| 4 |
NC_000001.11 |
1 |
202796240 |
- |
KDM5B |
NNNCCATTGTAGTACAGGAT |
NGG |
2 |
0.084 |
Tier II |
| 5 |
NC_000018.10 |
18 |
45385625 |
+ |
SLC14A2 |
NNNCCATTATAGTACATGGT |
NGC |
2 |
0.0143 |
Tier II |
| 6 |
NC_000002.12 |
2 |
99837931 |
- |
AFF3 |
NNNCCATTGTGGTACTTGGT |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000005.10 |
5 |
12484141 |
- |
LOC105374655 |
NNNATATTGTAGTACATGGT |
NGG |
2 |
0.5359 |
Tier III |
| 8 |
NC_000001.11 |
1 |
202796240 |
- |
SLC25A39P1 |
NNNCCATTGTAGTACAGGAT |
NGG |
2 |
0.084 |
Tier III |
| 9 |
NC_000009.12 |
9 |
110279621 |
+ |
LOC124902246 |
NNNTCATTGTAGTACATGGT |
NTG |
2 |
0.0312 |
Tier III |
| 10 |
NC_000018.10 |
18 |
45385625 |
+ |
SLC14A2-AS1 |
NNNCCATTATAGTACATGGT |
NGC |
2 |
0.0143 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
154594599 |
+ |
Tyw3 |
NNNGCATTGTAGAACATGGT |
NGG |
2 |
0.3462 |
Tier II |
| 2 |
NC_000083.6 |
17 |
25749975 |
+ |
Msln |
NNNACATTGTAGGACATGGT |
NGG |
2 |
0.2197 |
Tier II |
| 3 |
NC_000071.6 |
5 |
13453173 |
+ |
Sema3a |
NNNCCATGGTAGTCCATGGT |
NGG |
2 |
0.1571 |
Tier II |
| 4 |
NC_000074.6 |
8 |
69084358 |
+ |
Slc18a1 |
NNNCCAGTGTAGTAGATGGT |
NGG |
2 |
0.0294 |
Tier II |
| 5 |
NC_000072.6 |
6 |
113243227 |
+ |
Mtmr14 |
NNNCCATTGTAGTACATGCT |
NGT |
2 |
0.0072 |
Tier II |
Other clones with same target sequence:
(none)
