Construct: sgRNA BRDN0001148373
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TAAGTCAGGGGAAACGATTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PDGFRA (5156)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75493
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
54264982 |
+ |
PDGFRA |
NNNGTCAGGGGAAACGATTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
121109268 |
- |
TECTA |
NNNGTCAGGGGAAAGGATTC |
NGG |
2 |
0.0214 |
Tier I |
| 3 |
NC_000011.10 |
11 |
121109268 |
- |
TBCEL-TECTA |
NNNGTCAGGGGAAAGGATTC |
NGG |
2 |
0.0214 |
Tier I |
| 4 |
NC_000001.11 |
1 |
237345831 |
- |
RYR2 |
NNNGTCAGGGGAAAAGAATG |
NGG |
2 |
0.1481 |
Tier II |
| 5 |
NC_000016.10 |
16 |
89671 |
- |
NPRL3 |
NNNGTCAGGGGAGACGCTTG |
NGG |
2 |
0.1151 |
Tier II |
| 6 |
NC_000011.10 |
11 |
65829635 |
- |
LOC124902693 |
NNNGTCAGGGGAGACGGTTG |
NGG |
2 |
0.1151 |
Tier II |
| 7 |
NC_000020.11 |
20 |
48779852 |
+ |
PREX1 |
NNNGTCAGGGGTAACGATGG |
NGG |
2 |
0.0833 |
Tier II |
| 8 |
NC_000013.11 |
13 |
112841826 |
- |
ATP11A |
NNNGTCAGGGGAAACGTTTG |
NGT |
2 |
0.0022 |
Tier II |
| 9 |
NC_000015.10 |
15 |
65731928 |
- |
DENND4A |
NNNATCAGGGGAAACCATTG |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
48989430 |
+ |
Svs1 |
NNNGTCTGGGGAAAGGATTG |
NGG |
2 |
0.0219 |
Tier II |
| 2 |
NC_000082.6 |
16 |
92710906 |
+ |
Runx1 |
NNNGTCAGGGGAAAATATTG |
NGG |
2 |
0.0 |
Tier II |
| 3 |
NC_000083.6 |
17 |
67752316 |
- |
Lama1 |
NNNGTCAGGGGAAACCATTG |
NTG |
2 |
0.0 |
Tier II |
| 4 |
NC_000073.6 |
7 |
113975907 |
- |
Spon1 |
NNNGTCAGGGGAAACTTTTG |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000072.6 |
6 |
48989430 |
+ |
4930563H07Rik |
NNNGTCTGGGGAAAGGATTG |
NGG |
2 |
0.0219 |
Tier III |
Other clones with same target sequence:
(none)
