Construct: sgRNA BRDN0001148386
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGCTGGGCCTTATGCCACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAMKK1 (84254)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76515
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
3885412 |
- |
CAMKK1 |
NNNCTGGGCCTTATGCCACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
160395491 |
- |
IFT80 |
NNNCTGGGCCTCATGCCACA |
NGG |
2 |
0.8882 |
Tier II |
| 3 |
NC_000016.10 |
16 |
87641806 |
+ |
JPH3 |
NNNCTGGGCCATATGCCACA |
NGG |
2 |
0.7031 |
Tier II |
| 4 |
NC_000009.12 |
9 |
130225456 |
- |
NCS1 |
NNNCTTGGCCTTAAGCCACG |
NGG |
2 |
0.4127 |
Tier II |
| 5 |
NC_000023.11 |
X |
152120133 |
+ |
LOC100533997 |
NNNCTGGGGCTTAAGCCACG |
NGG |
2 |
0.3832 |
Tier II |
| 6 |
NC_000007.14 |
7 |
102475008 |
+ |
POLR2J |
NNNCTGGGCCTTGTGCCATG |
NGG |
2 |
0.301 |
Tier II |
| 7 |
NC_000007.14 |
7 |
102568658 |
+ |
POLR2J3 |
NNNCTGGGCCTTGTGCCATG |
NGG |
2 |
0.301 |
Tier II |
| 8 |
NC_000007.14 |
7 |
102667738 |
+ |
POLR2J2 |
NNNCTGGGCCTTGTGCCATG |
NGG |
2 |
0.301 |
Tier II |
| 9 |
NC_000007.14 |
7 |
99400276 |
- |
PDAP1 |
NNNCTGGGCCTTGTGTCACG |
NGG |
2 |
0.2007 |
Tier II |
| 10 |
NC_000017.11 |
17 |
82920319 |
+ |
TBCD |
NNNATGGGCCTTCTGCCACG |
NGG |
2 |
0.1773 |
Tier II |
| 11 |
NC_000021.9 |
21 |
45835845 |
+ |
PCBP3 |
NNNCTGGGCCTTATGCCCTG |
NGG |
2 |
0.0879 |
Tier II |
| 12 |
NC_000003.12 |
3 |
42655039 |
+ |
ZBTB47 |
NNNCTGGGCCTTGGGCCACG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000003.12 |
3 |
160395491 |
- |
TRIM59-IFT80 |
NNNCTGGGCCTCATGCCACA |
NGG |
2 |
0.8882 |
Tier III |
| 14 |
NC_000001.11 |
1 |
244321845 |
- |
LOC105373262 |
NNNCTGGGCCTTATGCCAAA |
NGG |
2 |
0.4018 |
Tier III |
| 15 |
NC_000023.11 |
X |
152120133 |
+ |
LOC105373369 |
NNNCTGGGGCTTAAGCCACG |
NGG |
2 |
0.3832 |
Tier III |
| 16 |
NC_000007.14 |
7 |
44015257 |
+ |
POLR2J4 |
NNNCTGGGCCTTGTGCCATG |
NGG |
2 |
0.301 |
Tier III |
| 17 |
NC_000007.14 |
7 |
102568658 |
+ |
POLR2J3-UPK3BL2 |
NNNCTGGGCCTTGTGCCATG |
NGG |
2 |
0.301 |
Tier III |
| 18 |
NC_000007.14 |
7 |
102667738 |
+ |
POLR2J2-UPK3BL1 |
NNNCTGGGCCTTGTGCCATG |
NGG |
2 |
0.301 |
Tier III |
| 19 |
NC_000021.9 |
21 |
45835845 |
+ |
PCBP3-AS1 |
NNNCTGGGCCTTATGCCCTG |
NGG |
2 |
0.0879 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
165139210 |
+ |
Tbx19 |
NNNCTGAGCCTGATGCCACG |
NGG |
2 |
0.5 |
Tier I |
| 2 |
NC_000077.6 |
11 |
115782670 |
+ |
Tmem94 |
NNNCTGGATCTTATGCCACG |
NGG |
2 |
0.875 |
Tier II |
| 3 |
NC_000072.6 |
6 |
55460576 |
+ |
Adcyap1r1 |
NNNCTGGTCCTCATGCCACG |
NGG |
2 |
0.5921 |
Tier II |
| 4 |
NC_000075.6 |
9 |
111644325 |
- |
Stac |
NNNCTGGGCCTCAAGCCACG |
NGG |
2 |
0.5865 |
Tier II |
| 5 |
NC_000069.6 |
3 |
83126692 |
+ |
Gm10710 |
NNNCTGGGGCTTATGCCACA |
NGG |
2 |
0.5804 |
Tier II |
| 6 |
NC_000076.6 |
10 |
82992107 |
+ |
Chst11 |
NNNCTGTGCCTTATGACACG |
NGG |
2 |
0.5714 |
Tier II |
| 7 |
NC_000074.6 |
8 |
108811899 |
- |
Zfhx3 |
NNNCTGGGCCCTATGCCACT |
NGG |
2 |
0.4667 |
Tier II |
| 8 |
NC_000074.6 |
8 |
92976634 |
+ |
Slc6a2 |
NNNCTGGGCCTGAAGCCACG |
NGG |
2 |
0.3095 |
Tier II |
| 9 |
NC_000081.6 |
15 |
78169476 |
- |
Ift27 |
NNNCTGGGCCATATGCCCCG |
NGG |
2 |
0.1429 |
Tier II |
| 10 |
NC_000074.6 |
8 |
125223563 |
+ |
Disc1 |
NNNCTGGGCATTATGCCAGG |
NGG |
2 |
0.1083 |
Tier II |
| 11 |
NC_000074.6 |
8 |
83939587 |
+ |
Adgrl1 |
NNNCTGGGCCTTATACCACG |
NGA |
2 |
0.0654 |
Tier II |
| 12 |
NC_000074.6 |
8 |
83939587 |
+ |
Gm10644 |
NNNCTGGGCCTTATACCACG |
NGA |
2 |
0.0654 |
Tier III |
| 13 |
NC_000078.6 |
12 |
77001250 |
+ |
Gm35041 |
NNNCTGGGCCTTCTGCCACG |
NAG |
2 |
0.0546 |
Tier III |
Other clones with same target sequence:
(none)
