Construct: sgRNA BRDN0001148391
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAGGAGCTTAGCCATGTCGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PDK2 (5164)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75687
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
50107085 |
- |
PDK2 |
NNNGAGCTTAGCCATGTCGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
29322350 |
+ |
MTUS2 |
NNNGAGCTTAGCCATATAGT |
NGG |
2 |
0.5385 |
Tier II |
| 3 |
NC_000008.11 |
8 |
18853727 |
- |
PSD3 |
NNNGAGCTTAGTCATGTAGT |
NGG |
2 |
0.2899 |
Tier II |
| 4 |
NC_000001.11 |
1 |
1832339 |
- |
GNB1 |
NNNGAGCTTAGCCGTGACGT |
NGG |
2 |
0.2489 |
Tier II |
| 5 |
NC_000009.12 |
9 |
128674784 |
+ |
DYNC2I2 |
NNNGAGTTTAGCCATGTCGT |
NCG |
2 |
0.0871 |
Tier II |
| 6 |
NC_000014.9 |
14 |
69954692 |
+ |
SMOC1 |
NNNGAGCTTAGCCAAGTGGT |
NGG |
2 |
0.0772 |
Tier II |
| 7 |
NC_000022.11 |
22 |
17146217 |
+ |
HDHD5 |
NNNGAGCTTAGCCATCTCCT |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000004.12 |
4 |
43251659 |
- |
LOC105374432 |
NNNCAGCTTAGCCATGTCAT |
NGG |
2 |
0.3782 |
Tier III |
| 9 |
NC_000001.11 |
1 |
110299820 |
+ |
RBM15-AS1 |
NNNGAGCTTAGCCATGGGGT |
NGG |
2 |
0.0157 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
95029723 |
+ |
Pdk2 |
NNNGAGCTTAGCCATGTCAT |
NGG |
1 |
0.7143 |
Tier I |
| 2 |
NC_000075.6 |
9 |
65398569 |
+ |
Rasl12 |
NNNCAGCTTCGCCATGTCGT |
NGG |
2 |
0.1765 |
Tier I |
| 3 |
NC_000068.7 |
2 |
33119229 |
+ |
Garnl3 |
NNNGAGATTAGCCATGCCGT |
NGG |
2 |
0.5294 |
Tier II |
| 4 |
NC_000083.6 |
17 |
47771410 |
+ |
Tfeb |
NNNGACCTTAGGCATGTCGT |
NGG |
2 |
0.303 |
Tier II |
| 5 |
NC_000075.6 |
9 |
43198582 |
- |
Pou2f3 |
NNNGAGCTTTGCCATGTGGT |
NGG |
2 |
0.1176 |
Tier II |
| 6 |
NC_000075.6 |
9 |
121320487 |
+ |
Trak1 |
NNNCAGCTTAGCCATGTCGC |
NGG |
2 |
0.0481 |
Tier II |
| 7 |
NC_000084.6 |
18 |
24631876 |
+ |
Elp2 |
NNNGAGCTTAGCCATGTGGT |
NTG |
2 |
0.0052 |
Tier II |
Other clones with same target sequence:
(none)
