Construct: sgRNA BRDN0001148392

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACAGTGTATAAACTCCCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBK1 (29110)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76362

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 64464379 - TBK1 NNNGTGTATAAACTCCCACA NGG 0 1.0 Tier I
2 NC_000006.12 6 161370079 - PRKN NNNGTGTACAAACTCCCACA NGG 1 0.9231 Tier II
3 NC_000008.11 8 86618381 + CNGB3 NNNGTGTAGAAACTCCCACA NGG 1 0.619 Tier II
4 NC_000002.12 2 144057781 + GTDC1 NNNGTGAAGAAACTCCCACA NGG 2 0.5417 Tier II
5 NC_000002.12 2 46360416 - EPAS1 NNNGAGCATAAACTCCCACA NGG 2 0.3438 Tier II
6 NC_000009.12 9 18640459 - ADAMTSL1 NNNGTGTATTAACTCTCACA NGG 2 0.2715 Tier II
7 NC_000001.11 1 203161510 - ADORA1 NNNGTGCATAAAATCCCACA NGG 2 0.2644 Tier II
8 NC_000001.11 1 241505812 + FH NNNCTGTATAAACTCCTACA NGG 2 0.2471 Tier II
9 NC_000012.12 12 21003261 - SLCO1B3-SLCO1B7 NNNGTGTATACACTCCCACT NGG 2 0.24 Tier II
10 NC_000012.12 12 21003261 - LOC124902894 NNNGTGTATACACTCCCACT NGG 2 0.24 Tier II
11 NC_000008.11 8 140124780 + TRAPPC9 NNNGGGTATAATCTCCCACA NGG 2 0.2133 Tier II
12 NC_000010.11 10 106878261 - SORCS1 NNNGTTTATAACCTCCCACA NGG 2 0.1754 Tier II
13 NC_000013.11 13 36203877 - SOHLH2 NNNCTGTATAAACTCCCACA NAG 2 0.1373 Tier II
14 NC_000013.11 13 36203877 - CCDC169-SOHLH2 NNNCTGTATAAACTCCCACA NAG 2 0.1373 Tier II
15 NC_000011.10 11 3968097 - STIM1 NNNGAGTATAAACTCCCAGA NGG 2 0.0625 Tier II
16 NC_000003.12 3 57422709 - DNAH12 NNNGAGTATAAACTCCCACA NGA 2 0.0347 Tier II
17 NC_000008.11 8 105744232 - ZFPM2 NNNGAGTATAAACTTCCACA NGG 2 0.0333 Tier II
18 NC_000015.10 15 37934974 + TMCO5A NNNTTGTATAAACTTCCACA NGG 2 0.0242 Tier II
19 NC_000009.12 9 108066884 - LOC105376214 NNNCTGTATAAACTCCCTCA NGG 2 0.2647 Tier III
20 NC_000013.11 13 111895513 - LINC00354 NNNCTGTATATACTCCCACA NGG 2 0.1629 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 121576359 + Tbk1 NNNGTGTATAAACTCCCACA NGG 0 1.0 Tier I
2 NC_000070.6 4 55011964 + Zfp462 NNNGTGTATCTACTCCCACA NGG 2 0.1026 Tier I
3 NC_000069.6 3 117677023 + Plppr5 NNNGTGCATAAACTCACACA NGG 2 0.6875 Tier II
4 NC_000070.6 4 141984428 + Fhad1 NNNGTGTATAAGCACCCACA NGG 2 0.4471 Tier II
5 NC_000073.6 7 37656309 - Zfp536 NNNGAGTAGAAACTCCCACA NGG 2 0.3095 Tier II
6 NC_000073.6 7 79927573 + Arpin NNNATGTATAAACTCCCACC NGG 2 0.2045 Tier II
7 NC_000072.6 6 141499163 + Pde3a NNNGTGTATAAATTCCCACA NAG 2 0.1815 Tier II
8 NC_000079.6 13 66918797 + Mterf3 NNNGTGTCTAACCTCCCACA NGG 2 0.1128 Tier II
9 NC_000082.6 16 34452586 - Kalrn NNNGTGTCTAACCTCCCACA NGG 2 0.1128 Tier II
10 NC_000079.6 13 19079114 - Amph NNNGTGTATACACTCCCACA NAG 2 0.1037 Tier II
11 NC_000073.6 7 4368045 - Gp6 NNNGTGTATAAACATCCACA NGG 2 0.0413 Tier II
12 NC_000072.6 6 54958188 - Nod1 NNNCTGTATAAACTGCCACA NGG 2 0.0265 Tier II
13 NC_000067.6 1 40830004 + Tmem182 NNNGTGTATAGACTCCCACA NGC 2 0.0144 Tier II
14 NC_000072.6 6 141251252 - Pde3a NNNGTGTATAAACGCCCACG NGG 2 0.0 Tier II
15 NC_000070.6 4 141984428 + Fhad1os1 NNNGTGTATAAGCACCCACA NGG 2 0.4471 Tier III
16 NC_000073.6 7 71689020 - Gm44689 NNNGGTTATAAACTCCCACA NGG 2 0.4267 Tier III
17 NC_000083.6 17 94756049 + 2700099C18Rik NNNGTGCATGAACTCCCACA NGG 2 0.3819 Tier III
18 NC_000077.6 11 79323637 + Gm30561 NNNGTGTGTACACTCCCACA NGG 2 0.2933 Tier III
19 NC_000077.6 11 43679203 - Gm34963 NNNCTGTATAATCTCCCACA NGG 2 0.1765 Tier III
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Other clones with same target sequence:

(none)