Construct: sgRNA BRDN0001148404
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCAGCCATCTGCTCTCGTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LATS1 (9113)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76023
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
149695129 |
+ |
LATS1 |
NNNGCCATCTGCTCTCGTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
24989560 |
- |
LOC102723675 |
NNNGCCATTTGCTCTCGTCG |
NAG |
2 |
0.2269 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
7697595 |
- |
Lats1 |
NNNGACATCTGCTCTCGACG |
NGG |
2 |
0.381 |
Tier I |
| 2 |
NC_000071.6 |
5 |
115524809 |
+ |
Pxn |
NNNGCCATGTGCTCTCATCG |
NGG |
2 |
0.5778 |
Tier II |
| 3 |
NC_000076.6 |
10 |
88772126 |
- |
Utp20 |
NNNGCTATCTGCTCTCGTAG |
NGG |
2 |
0.398 |
Tier II |
| 4 |
NC_000071.6 |
5 |
115964907 |
+ |
Cit |
NNNGCCATCTGCCCTCGGCG |
NGG |
2 |
0.2632 |
Tier II |
| 5 |
NC_000069.6 |
3 |
27153535 |
- |
Ect2 |
NNNGCCCTCTGCTCTCGTCC |
NGG |
2 |
0.1875 |
Tier II |
| 6 |
NC_000070.6 |
4 |
140069976 |
+ |
Igsf21 |
NNNGCCATCTGCTCTCCTCG |
NTG |
2 |
0.0092 |
Tier II |
| 7 |
NC_000086.7 |
X |
47471879 |
+ |
Gm4908 |
NNNGCCATCTGCTCTCCTCT |
NGG |
2 |
0.1647 |
Tier III |
| 8 |
NC_000081.6 |
15 |
75856158 |
- |
Gm9568 |
NNNGCCATCTGCTCTCCACG |
NGG |
2 |
0.1569 |
Tier III |
Other clones with same target sequence:
(none)
