Construct: sgRNA BRDN0001148417
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGATGAAATGACAGGCTACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAPK14 (1432)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77924
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
36075897 |
+ |
MAPK14 |
NNNTGAAATGACAGGCTACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
21145409 |
+ |
DNAH3 |
NNNTGACATGACAGTCTACG |
NGG |
2 |
0.0625 |
Tier I |
| 3 |
NC_000018.10 |
18 |
52474523 |
+ |
DCC |
NNNTAAAATGGCAGGCTACG |
NGG |
2 |
0.5633 |
Tier II |
| 4 |
NC_000012.12 |
12 |
22518386 |
+ |
C2CD5 |
NNNTGAAATGACAGACTAAG |
NGG |
2 |
0.4034 |
Tier II |
| 5 |
NC_000023.11 |
X |
85084478 |
+ |
APOOL |
NNNTCAAATGACAGGCTAAG |
NGG |
2 |
0.3367 |
Tier II |
| 6 |
NC_000002.12 |
2 |
153114656 |
+ |
GALNT13 |
NNNTGAAATGACAGGCTAAT |
NGG |
2 |
0.3 |
Tier II |
| 7 |
NC_000020.11 |
20 |
9757891 |
- |
PAK5 |
NNNTGTAATGACAGGTTACG |
NGG |
2 |
0.2198 |
Tier II |
| 8 |
NC_000008.11 |
8 |
54799444 |
- |
RP1 |
NNNTGAAATGACAGGCTGCC |
NGG |
2 |
0.1714 |
Tier II |
| 9 |
NC_000023.11 |
X |
110694969 |
+ |
CHRDL1 |
NNNTTAAATGACAGGCTAAG |
NGG |
2 |
0.1286 |
Tier II |
| 10 |
NC_000013.11 |
13 |
113599019 |
+ |
TFDP1 |
NNNTGAAATGACAGTCAACG |
NGG |
2 |
0.0762 |
Tier II |
| 11 |
NC_000001.11 |
1 |
15425585 |
- |
EFHD2 |
NNNTGAAATGACAGGCTCCG |
NGT |
2 |
0.0031 |
Tier II |
| 12 |
NC_000012.12 |
12 |
60119465 |
+ |
LOC124903070 |
NNNTGAAATGAAAGGCCACG |
NGG |
2 |
0.5042 |
Tier III |
| 13 |
NC_000003.12 |
3 |
107857053 |
- |
LINC00635 |
NNNTGAAATGACAGGCCACT |
NGG |
2 |
0.4941 |
Tier III |
| 14 |
NC_000009.12 |
9 |
2952275 |
- |
CARM1P1 |
NNNTGAAATGACAGGCTACC |
NGA |
2 |
0.0298 |
Tier III |
| 15 |
NC_000012.12 |
12 |
91427505 |
+ |
LOC105369896 |
NNNTGAAATGTCAGGCTACG |
NGT |
2 |
0.005 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
28728406 |
+ |
Mapk14 |
NNNTGAGATGACAGGCTACG |
NGG |
1 |
0.7059 |
Tier I |
| 2 |
NC_000068.7 |
2 |
4473156 |
- |
Frmd4a |
NNNTGAAATGAGAGGCCACG |
NGG |
2 |
0.3137 |
Tier I |
| 3 |
NC_000069.6 |
3 |
100930614 |
+ |
Trim45 |
NNNTGAAATGACAGGCTTCA |
NGG |
2 |
0.4688 |
Tier II |
| 4 |
NC_000076.6 |
10 |
4328449 |
+ |
Akap12 |
NNNTGAAATGACAGGCTCCG |
NGG |
1 |
0.1905 |
Tier II |
| 5 |
NC_000075.6 |
9 |
21430879 |
+ |
Dnm2 |
NNNTGAAATGACAGGCTCCT |
NGG |
2 |
0.1333 |
Tier II |
| 6 |
NC_000070.6 |
4 |
109228290 |
+ |
Calr4 |
NNNAGAAATGACAGGCTCCG |
NGG |
2 |
0.0907 |
Tier II |
| 7 |
NC_000068.7 |
2 |
31616456 |
- |
Fubp3 |
NNNTGAAATGCCAGGCTCCG |
NGG |
2 |
0.0762 |
Tier II |
| 8 |
NC_000083.6 |
17 |
14860452 |
+ |
Wdr27 |
NNNTGAAATGACAGGCTAAG |
NGA |
2 |
0.0298 |
Tier II |
| 9 |
NC_000068.7 |
2 |
4473156 |
- |
Gm39751 |
NNNTGAAATGAGAGGCCACG |
NGG |
2 |
0.3137 |
Tier III |
| 10 |
NC_000070.6 |
4 |
109228290 |
+ |
Gm40225 |
NNNAGAAATGACAGGCTCCG |
NGG |
2 |
0.0907 |
Tier III |
Other clones with same target sequence:
(none)
